The core technology of this Repository of Glyco-Enzyme Expression Constructs is the generation of recombinant forms of the glycosylation enzymes in soluble forms when possible. Each of the glycosylation enzymes has a different strategy for its subcellular localication, including in many cases membrane anchoring sequences.
Summary of the glycosylation enzyme domain structures and topologies.
For each class of GT or GH sequence, truncation strategies were developed when possible to remove transmembrane segments to yield catalytic domains that could presumably be expressed in a soluble forms.
Summary of fusion protein strategies in mammalian and baculovirus vectors in the Repository.