Gene Symbol | Uniprot | DNA RefSeq | Protein RefSeq | GeneID | MGC Acc# | CAZy Family |
POMGNT1 | Q8WZA1 | NM_017739 | NP_060209 | 55624 | BC001471 | GT13 |
GenBank Annotation | Domain structure | Truncation strategy (eukaryotic) |
Homo sapiens protein O-linked mannose beta1,2-N-acetylglucosaminyltransferase (POMGNT1), mRNA. | Type II TMD | N-term del/N-term fusion |
Construct Design
Gateway vector clone | Fusion Strategy | Construct info | DNASU Clone ID |
N-term pDONR221 capture clone | N-term fusion | GenBank Sequence | HsCD00358413 |
C-term pDONR221 capture clone | C-term fusion |
Expression Host | Vector | Fusion Strategy | Construct info | DNASU Clone ID |
Bacterial Expression | pET16-DEST | N-term fusion | ||
pET32-DEST | N-term fusion | |||
Mammalian Expression | pGEn1 | N-term fusion | GenBank Sequence | HsCD00413376 |
pGEn2 | N-term fusion | GenBank Sequence | HsCD00413191 | |
pGEn3 | N-term fusion | GenBank Sequence | HsCD00522517 | |
pGEc1 | C-term fusion | |||
pGEc2 | C-term fusion | |||
Baculovirus Expression | Ac-polh-CtermHisStrep-DEST | C-term fusion | Obtain virus stock from Jarvis lab HERE | |
Ac-polh-NtermMelHisStrep-DEST | N-term fusion | GenBank Sequence | Obtain virus stock from Jarvis lab HERE |
View graphic representation of MAMMALIAN EXPRESSION VECTOR DESIGNS (see HERE for PDF version) or BACULOVIRUS VECTOR DESIGNS (see HERE for PDF version)
For additional Gateway destination expression vectors please see the vector page HERE.
N.A.: not applicable