Custom expression vectors were generated for each recombinant host allowing transfer of Gateway® catalytic domain inserts from pDONR221 into the respective host-specific expression vector with appropriate protein fusions at either the N- or C-terminus. Below are the lists of vectors for each of the recombinant hosts.
Bacterial Expression Vectors (N-terminal fusions):
pET16-DEST: Vector Map, GenBankSequence, Sequence(PDF)
pET-32-DEST: Vector Map, GenBankSequence, Sequence(PDF)
Baculovirus Expression Vectors
Ac-polh-CtermHisStrep-DEST (C-terminal fusions): Vector Map, GenBank Sequence, Sequence (PDF)
Ac-polh-NtermMelHisStrep-DEST (N-terminal fusions): Vector Map, GenBank Sequence, Sequence (PDF)
Ac-polh-NtermMelHisAviTagGFP-DEST (N-terminal fusions): Vector Map, GenBank Sequence, Sequence (PDF)
Mammalian Expression Vectors
N-term fusion vectors:
pGEn1-DEST: |
Vector map | Genbank Sequence | Sequence (PDF) |
| pGEn2-DEST: Vector backbone, pXKM Sequences/Tags: N-term signal sequence, His tag, AviTag, superfolder GFP Insertion sites: Gateway® recombination site |
Vector map | Genbank Sequence | Sequence (PDF) |
| pGEn3-DEST: Vector backbone, pXKM Sequences/Tags: N-term signal sequence, AviTag, superfolder GFP, Fc fragment Insertion sites: Gateway® recombination site |
Vector map | Genbank Sequence | Sequence (PDF) |
C-term fusion vectors:
pGEc1-DEST: |
Vector map | Genbank Sequence | Sequence (PDF) |
| pGEc2-DEST: Vector backbone, pXKM Sequences/Tags: C-term superfolder GFP, AviTag, His tag, Term codon Insertion sites: Gateway® recombination site |
Vector map | Genbank Sequence | Sequence (PDF) |