Gene Symbol Uniprot DNA RefSeq Protein RefSeq GeneID MGC Acc# CAZy Family
GAA P10253 NM_000152 NP_000143  2548 BC040431  GH31
UniProt Protein Name Domain structure Truncation strategy (eukaryotic)
Lysosomal alpha-glucosidase N-term sig seq N-term deletion/N-term fusion

Construct Design

Gateway vector clone Fusion Strategy Construct info DNASU Clone ID
N-term pDONR221 capture clone N-term fusion GenBank Sequence HsCD00358409
C-term pDONR221 capture clone C-term fusion N.A. N.A.
Expression Host Vector Fusion Strategy Construct info DNASU Clone ID
Mammalian Expression pGEn1 N-term fusion GenBank Sequence HsCD00413372
  pGEn2 N-term fusion GenBank Sequence HsCD00413187
  pGEn3 N-term fusion GenBank Sequence HsCD00522513
  pGEc1 C-term fusion N.A. N.A.
  pGEc2 C-term fusion N.A.  N.A.
Baculovirus Expression Ac-polh-CtermHisStrep-DEST C-term fusion N.A. Obtain virus stock from Jarvis lab HERE
  Ac-polh-NtermMelHisStrep-DEST N-term fusion GenBank Sequence Obtain virus stock from Jarvis lab HERE

View graphic representation of MAMMALIAN EXPRESSION VECTOR DESIGNS (see HERE for PDF version) or BACULOVIRUS VECTOR DESIGNS (see HERE for PDF version)

For additional Gateway destination expression vectors please see the vector page HERE.

N.A.: not applicable