Primer 3 Output
WARNING: Numbers in input sequence were deleted.
PRIMER PICKING RESULTS FOR MGT M32
No mispriming library specified
Using 1-based sequence positions
OLIGO start len tm gc% any 3' seq
LEFT PRIMER 21 21 60.01 52.38 6.00 4.00 gaggaacactctgctgattgc
RIGHT PRIMER 110 20 59.42 45.00 4.00 2.00 aaggcatcgctgaagaaatc
SEQUENCE SIZE: 155
INCLUDED REGION SIZE: 155
PRODUCT SIZE: 90, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 4.00
1 cagtacccccatgcggtggggaggaacactctgctgattgccgggctccaggccaggaac
>>>>>>>>>>>>>>>>>>>>>
61 aacgcccgggtcatcttcagtggctctctggatttcttcagcgatgccttcttcaactcg
<<<<<<<<<<<<<<<<<<<<
121 gcagtgcagaaggccacacccggtgcgcagaggta
KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer
ADDITIONAL OLIGOS
start len tm gc% any 3' seq
1 LEFT PRIMER 19 20 59.26 55.00 4.00 2.00 gggaggaacactctgctgat
RIGHT PRIMER 106 21 59.96 47.62 4.00 2.00 catcgctgaagaaatccagag
PRODUCT SIZE: 88, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 3.00
2 LEFT PRIMER 19 20 59.26 55.00 4.00 2.00 gggaggaacactctgctgat
RIGHT PRIMER 107 19 59.90 47.37 4.00 0.00 gcatcgctgaagaaatcca
PRODUCT SIZE: 89, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00
3 LEFT PRIMER 21 21 60.01 52.38 6.00 4.00 gaggaacactctgctgattgc
RIGHT PRIMER 107 20 60.88 50.00 4.00 2.00 gcatcgctgaagaaatccag
PRODUCT SIZE: 87, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 4.00
4 LEFT PRIMER 19 20 59.26 55.00 4.00 2.00 gggaggaacactctgctgat
RIGHT PRIMER 104 21 59.70 47.62 4.00 0.00 tcgctgaagaaatccagagag
PRODUCT SIZE: 86, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 5.00
Statistics
con too in in no tm tm high high high
sid many tar excl bad GC too too any 3' poly end
ered Ns get reg GC% clamp low high compl compl X stab ok
Left 206 0 0 0 0 0 42 130 0 1 20 2 11
Right 237 0 0 0 0 0 56 149 0 0 0 7 25
Pair Stats:
considered 94, unacceptable product size 85, tm diff too large 2, ok 7
primer3 release 1.0
(primer3_www_results.cgi v 0.4)
Check Primers in Blast Search
LEFT PRIMER 21 21 60.01 52.38 6.00 4.00 gaggaacactctgctgattgc
RIGHT PRIMER 110 20 59.42 45.00 4.00 2.00 aaggcatcgctgaagaaatc
Primers OK to Test