Primer 3 Output

WARNING: Numbers in input sequence were deleted.
PRIMER PICKING RESULTS FOR MGT M31

No mispriming library specified
Using 1-based sequence positions
OLIGO start len tm gc% any 3' seq
LEFT PRIMER 124 20 60.01 55.00 3.00 0.00 ttcggctactgtctcctcgt
RIGHT PRIMER 200 20 59.87 50.00 4.00 2.00 aagctgcccacacaagagat
SEQUENCE SIZE: 211
INCLUDED REGION SIZE: 211

PRODUCT SIZE: 77, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 3.00

1 atgtcggcgtctgtggtgtccgtcatctcccggttcctggaggagtacttgagctccact

61 ccgcagcggctgaagttgctggacgcctatctcctttatatactgctgaccggggcgctg

121 cagttcggctactgtctcctcgtgggcaccttccccttcaactcgttcctctctggcttc
>>>>>>>>>>>>>>>>>>>>

181 atctcttgtgtgggcagcttcatcctagcgg
<<<<<<<<<<<<<<<<<<<<

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer

ADDITIONAL OLIGOS
start len tm gc% any 3' seq

1 LEFT PRIMER 120 20 60.02 60.00 4.00 3.00 gcagttcggctactgtctcc
RIGHT PRIMER 200 20 59.87 50.00 4.00 2.00 aagctgcccacacaagagat
PRODUCT SIZE: 81, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00

2 LEFT PRIMER 120 20 60.02 60.00 4.00 3.00 gcagttcggctactgtctcc
RIGHT PRIMER 196 20 59.83 50.00 2.00 0.00 tgcccacacaagagatgaag
PRODUCT SIZE: 77, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00

3 LEFT PRIMER 120 20 60.02 60.00 4.00 3.00 gcagttcggctactgtctcc
RIGHT PRIMER 197 20 59.83 50.00 2.00 0.00 ctgcccacacaagagatgaa
PRODUCT SIZE: 78, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00

4 LEFT PRIMER 10 20 59.67 55.00 3.00 3.00 tctgtggtgtccgtcatctc
RIGHT PRIMER 91 20 59.84 55.00 3.00 1.00 gataggcgtccagcaacttc
PRODUCT SIZE: 82, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00

Statistics
con too in in no tm tm high high high
sid many tar excl bad GC too too any 3' poly end
ered Ns get reg GC% clamp low high compl compl X stab ok
Left 371 0 0 0 0 0 112 209 0 0 20 4 26
Right 335 0 0 0 0 0 117 133 0 0 36 8 41
Pair Stats:
considered 138, unacceptable product size 130, ok 8
primer3 release 1.0

(primer3_www_results.cgi v 0.4)

First pair of primer used
Sense ttcggctactgtctcctcgt
Anti-sense aagctgcccacacaagagat

Second pair to test

2 LEFT PRIMER 120 20 60.02 60.00 4.00 3.00 gcagttcggctactgtctcc
RIGHT PRIMER 196 20 59.83 50.00 2.00 0.00 tgcccacacaagagatgaag
PRODUCT SIZE: 77, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00