1 atgatcacgg acgtgcagct cgccatcttc gccaacatgc tgggcgtgtc gcttttcttg
61 cttgtggtcc tctatcacta cgtggcagta aacaacccca agaagcagga atga

Primer3 results:

MGT M63

PRIMER PICKING RESULTS FOR MGT M63

No mispriming library specified
Using 1-based sequence positions
OLIGO start len tm gc% any 3' seq
LEFT PRIMER 33 17 60.27 58.82 6.00 5.00 CAACATGCTGGGCGTGT
RIGHT PRIMER 98 21 59.55 52.38 5.00 4.00 GGGTTGTTTACTGCCACGTAG
SEQUENCE SIZE: 114
INCLUDED REGION SIZE: 114

PRODUCT SIZE: 66, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00

1 ATGATCACGGACGTGCAGCTCGCCATCTTCGCCAACATGCTGGGCGTGTCGCTTTTCTTG
>>>>>>>>>>>>>>>>>

61 CTTGTGGTCCTCTATCACTACGTGGCAGTAAACAACCCCAAGAAGCAGGAATGA
<<<<<<<<<<<<<<<<<<<<<

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer

ADDITIONAL OLIGOS
start len tm gc% any 3' seq

1 LEFT PRIMER 34 17 59.15 58.82 5.00 3.00 AACATGCTGGGCGTGTC
RIGHT PRIMER 98 21 59.55 52.38 5.00 4.00 GGGTTGTTTACTGCCACGTAG
PRODUCT SIZE: 65, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00

Primers to test: OK in Blast search

VALIDATED PRIMER SEQUENCES:

LEFT PRIMER 33 17 60.27 58.82 6.00 5.00 CAACATGCTGGGCGTGT
RIGHT PRIMER 98 21 59.55 52.38 5.00 4.00 GGGTTGTTTACTGCCACGTAG