ag caggtttgga ggatggcctt ctagaagaat cccagtatga agacgtacca
1321 gaggaaatcc ccacctctca agaccagaat actgggatac aagggagaaa acagaagact
1381 atttccaccc cggggctggg tgtcactgac taccacctgc ggaagctctt ggctcgctca
1441 cagagtggcc ctgtagcgcc tctttccaaa cagaactcta caactgcctt tccaaccagg
1501 acaagcaaca tcccagtcca gcggccagag aaaagccctg tgcccagccg agatttgtct
1561 cattctgacc agggggcccg gaggaacctg cctctcatcc agagagccag gcccactggt
1621 gacagacctg ggaagactct tgagcagtcc cagtggctga atcaagtgga atccttcatt
1681 gctgagcaga gaaggggaga caggatagag cctccaaccc ccagcagggg ctggcgtcct
1741 gaggaggacg tggtgatagc ggcggaccag gaaggagaag tggaggagga ggaagagggg
1801 gaagatgagg aagaagatat gagtgaggtg ttcgaatatg tgcctatgtt tgacccagtg
1861 gtgaactggg gccagacctt cagcgctcag aacctcgact tccaagccct gagaaccgac
1921 tggatcgacc tgaactgtaa cacatcgggc aacctgctgc ttccggagca ggaggccctg
1981 gaggtcacac gggtcttcct gagaaagctc agccagagga cccggggg

Primer 3

PRIMER PICKING RESULTS FOR GT07 m10

No mispriming library specified
Using 1-based sequence positions
OLIGO start len tm gc% any 3' seq
LEFT PRIMER 276 20 60.22 50.00 4.00 2.00 ccagccgagatttgtctcat
RIGHT PRIMER 342 20 60.09 60.00 6.00 4.00 ctggctctctggatgagagg
SEQUENCE SIZE: 760
INCLUDED REGION SIZE: 760

PRODUCT SIZE: 67, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 4.00

1 agcaggtttggaggatggccttctagaagaatcccagtatgaagacgtaccagaggaaat

61 ccccacctctcaagaccagaatactgggatacaagggagaaaacagaagactatttccac

121 cccggggctgggtgtcactgactaccacctgcggaagctcttggctcgctcacagagtgg

181 ccctgtagcgcctctttccaaacagaactctacaactgcctttccaaccaggacaagcaa

241 catcccagtccagcggccagagaaaagccctgtgcccagccgagatttgtctcattctga
>>>>>>>>>>>>>>>>>>>>

301 ccagggggcccggaggaacctgcctctcatccagagagccaggcccactggtgacagacc
<<<<<<<<<<<<<<<<<<<<

361 tgggaagactcttgagcagtcccagtggctgaatcaagtggaatccttcattgctgagca

421 gagaaggggagacaggatagagcctccaacccccagcaggggctggcgtcctgaggagga

481 cgtggtgatagcggcggaccaggaaggagaagtggaggaggaggaagagggggaagatga

541 ggaagaagatatgagtgaggtgttcgaatatgtgcctatgtttgacccagtggtgaactg

601 gggccagaccttcagcgctcagaacctcgacttccaagccctgagaaccgactggatcga

661 cctgaactgtaacacatcgggcaacctgctgcttccggagcaggaggccctggaggtcac

721 acgggtcttcctgagaaagctcagccagaggacccggggg

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer

ADDITIONAL OLIGOS
start len tm gc% any 3' seq

1 LEFT PRIMER 574 20 60.38 55.00 5.00 5.00 gcctatgtttgacccagtgg
RIGHT PRIMER 639 20 59.99 55.00 4.00 1.00 gcttggaagtcgaggttctg
PRODUCT SIZE: 66, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00

2 LEFT PRIMER 573 20 59.57 50.00 3.00 3.00 tgcctatgtttgacccagtg
RIGHT PRIMER 639 20 59.99 55.00 4.00 1.00 gcttggaagtcgaggttctg
PRODUCT SIZE: 67, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 1.00

3 LEFT PRIMER 350 20 59.53 60.00 4.00 1.00 ggtgacagacctgggaagac
RIGHT PRIMER 419 20 59.78 50.00 5.00 5.00 gctcagcaatgaaggattcc
PRODUCT SIZE: 70, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 3.00

4 LEFT PRIMER 184 20 60.39 50.00 4.00 2.00 tgtagcgcctctttccaaac
RIGHT PRIMER 249 20 59.58 50.00 3.00 0.00 actgggatgttgcttgtcct
PRODUCT SIZE: 66, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00

Statistics
con too in in no tm tm high high high
sid many tar excl bad GC too too any 3' poly end
ered Ns get reg GC% clamp low high compl compl X stab ok
Left 1723 0 0 0 1 0 658 700 0 0 177 33 154
Right 1716 0 0 0 1 0 577 762 0 1 180 35 160
Pair Stats:
considered 1296, unacceptable product size 1284, ok 12
primer3 release 1.0

Primers Passed testing!

Forward: ccagccgagatttgtctcat

Reverse: ctggctctctggatgagagg