Primer3 Output

WARNING: Numbers in input sequence were deleted.
PRIMER PICKING RESULTS FOR gt66 m2

No mispriming library specified
Using 1-based sequence positions
OLIGO start len tm gc% any 3' seq
LEFT PRIMER 94 20 59.50 45.00 6.00 2.00 gaattccgtgttgatcgtga
RIGHT PRIMER 174 20 59.77 55.00 3.00 3.00 ctgcccaaagcggtagtaac
SEQUENCE SIZE: 190
INCLUDED REGION SIZE: 190

PRODUCT SIZE: 81, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00

1 gatatcaacaagtttctttggatggtccggattggaggaagcacagagacaggaagacac

61 attaaggagaatgactactatactcctactggggaattccgtgttgatcgtgagggttct
>>>>>>>>>>>>>>>>>>>>

121 ccggtgctgctcaactgccttatgtacaaaatgtgttactaccgctttgggcaggtctac
<<<<<<<<<<<<<<<<<<<<

181 acagaagcca

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer

ADDITIONAL OLIGOS
start len tm gc% any 3' seq

1 LEFT PRIMER 94 20 59.50 45.00 6.00 2.00 gaattccgtgttgatcgtga
RIGHT PRIMER 172 20 59.64 55.00 3.00 3.00 gcccaaagcggtagtaacac
PRODUCT SIZE: 79, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00

2 LEFT PRIMER 34 20 59.99 60.00 2.00 0.00 ggaggaagcacagagacagg
RIGHT PRIMER 115 20 60.92 50.00 4.00 2.00 cctcacgatcaacacggaat
PRODUCT SIZE: 82, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00

3 LEFT PRIMER 103 20 59.51 55.00 4.00 0.00 gttgatcgtgagggttctcc
RIGHT PRIMER 185 20 59.29 50.00 4.00 4.00 tctgtgtagacctgcccaaa
PRODUCT SIZE: 83, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 1.00

4 LEFT PRIMER 34 20 59.99 60.00 2.00 0.00 ggaggaagcacagagacagg
RIGHT PRIMER 118 19 59.54 52.63 4.00 2.00 aaccctcacgatcaacacg
PRODUCT SIZE: 85, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00

Statistics
con too in in no tm tm high high high
sid many tar excl bad GC too too any 3' poly end
ered Ns get reg GC% clamp low high compl compl X stab ok
Left 308 0 0 0 0 0 165 105 0 0 18 4 16
Right 277 0 0 0 0 0 114 107 0 1 36 5 14
Pair Stats:
considered 74, unacceptable product size 63, tm diff too large 3, ok 8
primer3 release 1.0

(primer3_www_results.cgi v 0.4)

Sequence 1: lcl|seq_1
Length = 42 (1 .. 42)

Sequence 2: lcl|seq_2
Length = 2118 (1 .. 2118)

2
1

NOTE:Bitscore and expect value are calculated based on the size of the nr database.

NOTE:If protein translation is reversed, please repeat the search with reverse strand of the query sequence.

Score = 41.1 bits (21), Expect = 0.14
Identities = 21/21 (100%), Gaps = 0/21 (0%)
Strand=Plus/Plus

Query 1 GATCGTGAGGGTTCTCCGGTG 21
|||||||||||||||||||||
Sbjct 1879 GATCGTGAGGGTTCTCCGGTG 1899

Score = 41.1 bits (21), Expect = 0.14
Identities = 21/21 (100%), Gaps = 0/21 (0%)
Strand=Plus/Minus

Query 22 TGTGTAGACCTGCCCAAAGCG 42
|||||||||||||||||||||
Sbjct 1956 TGTGTAGACCTGCCCAAAGCG 1936

CPU time: 0.03 user secs. 0.01 sys. secs 0.04 total secs.

Lambda K H
1.33 0.621 1.12

Gapped
Lambda K H
1.33 0.621 1.12

Matrix: blastn matrix:1 -2
Gap Penalties: Existence: 5, Extension: 2
Number of Sequences: 1
Number of Hits to DB: 7
Number of extensions: 2
Number of successful extensions: 2
Number of sequences better than 10.0: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 42
Length of database: 16,556,997,203
Length adjustment: 23
Effective length of query: 19
Effective length of database: 16,556,997,180
Effective search space: 314582946420
Effective search space used: 314582946420
X1: 11 (21.1 bits)
X2: 26 (50.0 bits)
X3: 26 (50.0 bits)
S1: 11 (21.8 bits)
S2: 18 (35.3 bits)

VALIDATED PRIMER SEQUENCES:

FORWARD: GATCGTGAGGGTTCTCCGGTG

REVERSE: TGTGTAGACCTGCCCAAAGCG