Checked:
ggcgtctac
901 ccaccgtatg ccgggggtgg tggattcctg tactccggcc cccttgcctt gaggctgtac
961 agtgcgacta gccggg

Primer3:
WARNING: Numbers in input sequence were deleted.
PRIMER PICKING RESULTS FOR GT31M16

No mispriming library specified
Using 1-based sequence positions
OLIGO start len tm gc% any 3' seq
LEFT PRIMER 683 20 60.11 55.00 4.00 2.00 ttaggtgggtgagcacttcc
RIGHT PRIMER 760 20 59.86 50.00 6.00 4.00 gatgggtgttcacaaacacg
SEQUENCE SIZE: 1194
INCLUDED REGION SIZE: 1194

PRODUCT SIZE: 78, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 3.00

1 atgagtgtggggcgtcgaagagtcaagttgctgggcatcctgatgatggcaaatgtcttc

61 atttatttgattgtggaagtctccaaaaacagtagccaagacaaaaatggaaagggagga

121 gtaataatcccgaaagagaagttctggaagccacccagcactccccgggcatactggaac

181 agggaacaggagaagctgaacaggtggtacaatcccatcttgaacagggtggccaatcag

241 acaggggagctagccacatctccaaacacaagtcacctgagctattgtgaaccagactcg

301 acggtcatgacagctgtgacagattttaataatctgccggacagatttaaagactttctc

361 ttgtatttgagatgccggaattactcgctgcttatagatcaaccgaagaaatgtgcaaag

421 aagcccttcttactattggcgataaagtccctcattccacattttgccagaaggcaagca

481 attcgggagtcttggggccgagaaaccaacgtagggaaccagacagtagtgagggtcttc

541 ctgttgggcaagacacccccagaggacaaccaccctgacctttcggacatgcttaagttt

601 gagagtgacaagcaccaggacatcctcatgtggaactatagagacacattcttcaacctg

661 tccctgaaggaagtgctgtttcttaggtgggtgagcacttcctgtccagacgcagagttt
>>>>>>>>>>>>>>>>>>>>

721 gtcttcaagggcgatgatgacgtgtttgtgaacacccatcacatccttaattacttgaat
<<<<<<<<<<<<<<<<<<<<

781 agcttatccaagagcaaagccaaagacttgttcataggtgacgtgatccacaatgctggg

841 cctcaccgggataagaaactgaagtactacatcccagaagtcttctacaccggcgtctac

901 ccaccgtatgccgggggtggtggattcctgtactccggcccccttgccttgaggctgtac

961 agtgcgactagccgggtccatctctaccctattgatgatgtttatacgggaatgtgcctt

1021 cagaaactgggccttgttccagagaagcacaaaggcttcaggacatttgatattgaagag

1081 aaaaataagaaaaatatttgttcctatatagacctaatgttagtacatagcagaaaacct

1141 caagagatgattgatatctggtctcagttgcaaagtcctaatttaaaatgctga

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer

ADDITIONAL OLIGOS
start len tm gc% any 3' seq

1 LEFT PRIMER 535 20 59.70 55.00 5.00 5.00 gtcttcctgttgggcaagac
RIGHT PRIMER 619 20 60.02 55.00 3.00 1.00 cctggtgcttgtcactctca
PRODUCT SIZE: 85, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00

2 LEFT PRIMER 683 20 60.11 55.00 4.00 2.00 ttaggtgggtgagcacttcc
RIGHT PRIMER 766 20 60.23 50.00 5.00 4.00 ggatgtgatgggtgttcaca
PRODUCT SIZE: 84, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 3.00

3 LEFT PRIMER 684 20 59.72 55.00 4.00 2.00 taggtgggtgagcacttcct
RIGHT PRIMER 760 20 59.86 50.00 6.00 4.00 gatgggtgttcacaaacacg
PRODUCT SIZE: 77, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 2.00

4 LEFT PRIMER 684 20 59.72 55.00 4.00 2.00 taggtgggtgagcacttcct
RIGHT PRIMER 766 20 60.23 50.00 5.00 4.00 ggatgtgatgggtgttcaca
PRODUCT SIZE: 83, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 3.00

Statistics
con too in in no tm tm high high high
sid many tar excl bad GC too too any 3' poly end
ered Ns get reg GC% clamp low high compl compl X stab ok
Left 2911 0 0 0 41 0 1697 627 2 0 215 60 269
Right 2887 0 0 0 38 0 1734 573 0 0 228 55 259
Pair Stats:
considered 2024, unacceptable product size 2010, ok 14
primer3 release 0.9

(primer3_www_results.cgi v 0.2)
Original sequence works.

VALIDATED PRIMERS:

LEFT PRIMER 683 20 60.11 55.00 4.00 2.00 ttaggtgggtgagcacttcc
RIGHT PRIMER 760 20 59.86 50.00 6.00 4.00 gatgggtgttcacaaacacg