Checked:
gg
901 gtcgtgcccg tgtattacgg atcccccacc atcgccgact ggcttccaag taaccgaagc
961 gccattctgg tgt
Primer3:
WARNING: Numbers in input sequence were deleted.
PRIMER PICKING RESULTS FOR GT10M05
No mispriming library specified
Using 1-based sequence positions
OLIGO start len tm gc% any 3' seq
LEFT PRIMER 744 20 60.24 55.00 5.00 1.00 gggtgtgcaggacattaacc
RIGHT PRIMER 827 20 60.18 55.00 4.00 4.00 agcctactgtttgcccacac
SEQUENCE SIZE: 936
INCLUDED REGION SIZE: 936
PRODUCT SIZE: 84, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 5.00
1 ggtactgactttaacatagacagcttacctttgcctcgggaagcccatcatgactgggcg
61 cttttccatgaagagtcccccaaaaacaattataagctcttccataaaccagtcatcacc
121 ttattcaaccatacggccacattcagcaggcattcccacttgccactgactacgcagtac
181 ctggaaggcgtggacgtcctgaagtcactcagatacctagttcctttacaggctaagaac
241 aacctaagacaaaaactggccccattggtatatgttcagtcggactgcgatccaccatca
301 gacagggacagctatgtccgggagctgatggcgtacattgaagttgattcctatggcgag
361 tgtttacagaacagagatcttccccagcagctgaaaaaccccgcctccatggatgctgat
421 gctttctacagagtcattgcccagtataagttcatcctggcctttgagaatgcggtctgt
481 gacgattacatcacagagaagttctggagaccactgaaactgggggtcgtgcccgtgtat
541 tacggatcccccaccatcgccgactggcttccaagtaaccgaagcgccattctggtgtcg
601 gaattctctcaccccagagagctggcgagcttcattaggagactggattatgacgatggg
661 ttgtatgagacctatgtagagtggaagctaaagggcaagatctctaaccagcggcttctg
721 acagctctcaatgaacgggagtggggtgtgcaggacattaaccaggacaactacattgac
>>>>>>>>>>>>>>>>>>>>
781 tcgtttgagtgtatggtgtgccgcagggtgtgggcaaacagtaggctgcaggagcaggtg
<<<<<<<<<<<<<<<<<<<<
841 agtgaatggaaatctgggggctggcatgggccgtctctctgtgtggtcctggtcttcctt
901 ctgtggtggttgcctgccacagggctctattcctag
KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer
ADDITIONAL OLIGOS
start len tm gc% any 3' seq
1 LEFT PRIMER 830 20 60.26 55.00 2.00 0.00 aggagcaggtgagtgaatgg
RIGHT PRIMER 906 20 60.23 55.00 3.00 1.00 ccacagaaggaagaccagga
PRODUCT SIZE: 77, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00
2 LEFT PRIMER 529 20 60.22 55.00 5.00 4.00 gtgcccgtgtattacggatc
RIGHT PRIMER 606 20 60.32 50.00 6.00 3.00 gaattccgacaccagaatgg
PRODUCT SIZE: 78, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
3 LEFT PRIMER 828 20 60.42 55.00 2.00 2.00 gcaggagcaggtgagtgaat
RIGHT PRIMER 903 20 60.23 55.00 3.00 1.00 cagaaggaagaccaggacca
PRODUCT SIZE: 76, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 1.00
4 LEFT PRIMER 828 20 60.42 55.00 2.00 2.00 gcaggagcaggtgagtgaat
RIGHT PRIMER 906 20 60.23 55.00 3.00 1.00 ccacagaaggaagaccagga
PRODUCT SIZE: 79, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 1.00
Statistics
con too in in no tm tm high high high
sid many tar excl bad GC too too any 3' poly end
ered Ns get reg GC% clamp low high compl compl X stab ok
Left 2171 0 0 0 0 0 1121 651 2 0 194 43 160
Right 2187 0 0 0 0 0 1104 665 2 0 182 43 191
Pair Stats:
considered 1713, unacceptable product size 1699, ok 14
primer3 release 0.9
(primer3_www_results.cgi v 0.2)
works.
Primers passed testing:
Forward Primer: GGGTGTGCAGGACATTAACC
Reverse Primer: AGCCTACTGTTTGCCCACAC