LOCUS       pDONR/Zeo
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FEATURES             Location/Qualifiers
     misc_feature    complement(268..295)
                     /note="rrnB T2 transcription termination sequence (c)"
     misc_feature    complement(427..470)
                     /note="rrnB T1 transcription termination sequence (c)"
     primer_bind     537..552
                     /note="M13 Forward (-20) priming site"
     misc_recomb     570..668
                     /label=attL1
                     /note="attL1"
     misc_recomb     complement(1664..1760)
                     /label=attL2
                     /note="attL2 (c)"
     misc_signal     complement(1775..1794)
                     /note="T7 Promoter/priming site (c)"
     primer_bind     1802..1818
                     /note="M13 Reverse priming site"
     gene            1931..2740
                     /note="Kanamycin resistance gene"
     rep_origin      2861..3534
                     /note="pUC origin"
     vector          join(1672..3537,1..651)
                     /source="pDONR%99221"
                     /type="Donor Vector"
     misc_feature    669..691
                     /note="TEV site"
     source          692..1657
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="MGC:103935 IMAGE:30915328"
                     /tissue_type="Lung, PCR rescued clones"
                     /clone_lib="NIH_MGC_265"
                     /lab_host="DH10B"
                     /note="Vector: pCR4 Topo TA"
     gene            692..1657
                     /gene="FUT3"
                     /gene_synonym=CD174
                     /gene_synonym=Les
                     /db_xref="GeneID:2525"
                     /db_xref="HGNC:4014"
                     /db_xref="MIM:111100"
     CDS             692..1657
                     /dnas_title="fucosyltransferase 3 (galactoside
                     3(4)-L-fucosyltransferase, Lewis blood group)"
                     /gene="FUT3"
                     /gene_synonym=CD174
                     /gene_synonym=Les
                     /codon_start=1
                     /product="fucosyltransferase 3 (galactoside
                     3(4)-L-fucosyltransferase, Lewis blood group)"
                     /protein_id="AAH74836.1"
                     /db_xref="GI:49902395"
                     /db_xref="GeneID:2525"
                     /db_xref="HGNC:4014"
                     /db_xref="MIM:111100"
                     /translation="MDPLGAAKPQWPWRRCLAALLFQLLVAVCFFSYLRVSRDDATGS
                     PRAPSGSSRQDTTPTRPTLLILLWTWPFHIPVALSRCSEMVPGTADCHITADRKVYPQ
                     ADTVIVHHWDIMSNPKSRLPPSPRPQGQRWIWFNLEPPPNCQHLEALDRYFNLTMSYR
                     SDSDIFTPYGWLEPWSGQPAHPPLNLSAKTELVAWAVSNWKPDSARVRYYQSLQAHLK
                     VDVYGRSHKPLPKGTMMETLSRYKFYLAFENSLHPDYITEKLWRNALEAWAVPVVLGP
                     SRSNYERFLPPDAFIHVDDFQSPKDLARYLQELDKDHARYLSYFRWRETLRPRSFSWA
                     LDFCKACWKLQQESRYQTVRSIAAWFT"
     misc_difference 776..776
                     /gene="FUT3"
                     /gene_synonym=CD174
                     /gene_synonym=Les
                     /note="'T' in cDNA is 'C' in the human genome; amino acid
                     difference: 'W' in cDNA, 'R' in the human genome.  The
                     chimpanzee genome agrees with the cDNA sequence,
                     suggesting that this difference is unlikely to be due to
                     an artifact."
     misc_difference 888..888
                     /gene="FUT3"
                     /gene_synonym=CD174
                     /gene_synonym=Les
                     /note="'C' in cDNA is 'T' in the human genome; amino acid
                     difference: 'T' in cDNA, 'M' in the human genome.  The
                     chimpanzee genome agrees with the cDNA sequence,
                     suggesting that this difference is unlikely to be due to
                     an artifact."
     misc_feature    692..1657
                     /note="Fut3"
ORIGIN      
          1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
         61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
        121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
        181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
        241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
        301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
        361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
        421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
        481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
        541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC
        601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA
        661 AGCAGGCTct gaaaacttgt actttcaagg cgatgccact ggatccccta gggctcccag
        721 tgggtcctcc cgacaggaca ccactcccac ccgccccacc ctcctgatcc tgctatggac
        781 atggcctttc cacatccctg tggctctgtc ccgctgttca gagatggtgc ccggcacagc
        841 cgactgccac atcactgccg accgcaaggt gtacccacag gcagacacgg tcatcgtgca
        901 ccactgggat atcatgtcca accctaagtc acgcctccca ccttccccga ggccgcaggg
        961 gcagcgctgg atctggttca acttggagcc accccctaac tgccagcacc tggaagccct
       1021 ggacagatac ttcaatctca ccatgtccta ccgcagcgac tccgacatct tcacgcccta
       1081 cggctggctg gagccgtggt ccggccagcc tgcccaccca ccgctcaacc tctcggccaa
       1141 gaccgagctg gtggcctggg cggtgtccaa ctggaagccg gactcagcca gggtgcgcta
       1201 ctaccagagc ctgcaggctc atctcaaggt ggacgtgtac ggacgctccc acaagcccct
       1261 gcccaagggg accatgatgg agacgctgtc ccggtacaag ttctacctgg ccttcgagaa
       1321 ctccttgcac cccgactaca tcaccgagaa gctgtggagg aacgccctgg aggcctgggc
       1381 cgtgcccgtg gtgctgggcc ccagcagaag caactacgag aggttcctgc cacccgacgc
       1441 cttcatccac gtggacgact tccagagccc caaggacctg gcccggtacc tgcaggagct
       1501 ggacaaggac cacgcccgct acctgagcta ctttcgctgg cgggagacgc tgcggcctcg
       1561 ctccttcagc tgggcactgg atttctgcaa ggcctgctgg aaactgcagc aggaatccag
       1621 gtaccagacg gtgcgcagca tagcggcttg gttcaccTAG GACCCAGCTT TCTTGTACAA
       1681 AGTTGGCATT ATAAGAAAGC ATTGCTTATC AATTTGTTGC AACGAACAGG TCACTATCAG
       1741 TCAAAATAAA ATCATTATTT GCCATCCAGC TGATATCCCC TATAGTGAGT CGTATTACAT
       1801 GGTCATAGCT GTTTCCTGGC AGCTCTGGCC CGTGTCTCAA AATCTCTGAT GTTACATTGC
       1861 ACAAGATAAA ATAATATCAT CATGAACAAT AAAACTGTCT GCTTACATAA ACAGTAATAC
       1921 AAGGGGTGTT ATGAGCCATA TTCAACGGGA AACGTCGAGG CCGCGATTAA ATTCCAACAT
       1981 GGATGCTGAT TTATATGGGT ATAAATGGGC TCGCGATAAT GTCGGGCAAT CAGGTGCGAC
       2041 AATCTATCGC TTGTATGGGA AGCCCGATGC GCCAGAGTTG TTTCTGAAAC ATGGCAAAGG
       2101 TAGCGTTGCC AATGATGTTA CAGATGAGAT GGTCAGACTA AACTGGCTGA CGGAATTTAT
       2161 GCCTCTTCCG ACCATCAAGC ATTTTATCCG TACTCCTGAT GATGCATGGT TACTCACCAC
       2221 TGCGATCCCC GGAAAAACAG CATTCCAGGT ATTAGAAGAA TATCCTGATT CAGGTGAAAA
       2281 TATTGTTGAT GCGCTGGCAG TGTTCCTGCG CCGGTTGCAT TCGATTCCTG TTTGTAATTG
       2341 TCCTTTTAAC AGCGATCGCG TATTTCGTCT CGCTCAGGCG CAATCACGAA TGAATAACGG
       2401 TTTGGTTGAT GCGAGTGATT TTGATGACGA GCGTAATGGC TGGCCTGTTG AACAAGTCTG
       2461 GAAAGAAATG CATAAACTTT TGCCATTCTC ACCGGATTCA GTCGTCACTC ATGGTGATTT
       2521 CTCACTTGAT AACCTTATTT TTGACGAGGG GAAATTAATA GGTTGTATTG ATGTTGGACG
       2581 AGTCGGAATC GCAGACCGAT ACCAGGATCT TGCCATCCTA TGGAACTGCC TCGGTGAGTT
       2641 TTCTCCTTCA TTACAGAAAC GGCTTTTTCA AAAATATGGT ATTGATAATC CTGATATGAA
       2701 TAAATTGCAG TTTCATTTGA TGCTCGATGA GTTTTTCTAA TCAGAATTGG TTAATTGGTT
       2761 GTAACACTGG CAGAGCATTA CGCTGACTTG ACGGGACGGC GCAAGCTCAT GACCAAAATC
       2821 CCTTAACGTG AGTTACGCGT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG
       2881 ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC
       2941 GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC
       3001 TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTTCTTCTA GTGTAGCCGT AGTTAGGCCA
       3061 CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT
       3121 GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC
       3181 GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG
       3241 AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC
       3301 CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC
       3361 GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT
       3421 CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC
       3481 CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTT
//
