LOCUS       pDONR/Zeo
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FEATURES             Location/Qualifiers
     misc_feature    complement(268..295)
                     /note="rrnB T2 transcription termination sequence (c)"
     misc_feature    complement(427..470)
                     /note="rrnB T1 transcription termination sequence (c)"
     primer_bind     537..552
                     /note="M13 Forward (-20) priming site"
     misc_recomb     570..668
                     /label=attL1
                     /note="attL1"
     misc_recomb     complement(1898..1994)
                     /label=attL2
                     /note="attL2 (c)"
     misc_signal     complement(2009..2028)
                     /note="T7 Promoter/priming site (c)"
     primer_bind     2036..2052
                     /note="M13 Reverse priming site"
     gene            2165..2974
                     /note="Kanamycin resistance gene"
     rep_origin      3095..3768
                     /note="pUC origin"
     vector          join(1906..3771,1..651)
                     /source="pDONR%99221"
                     /type="Donor Vector"
     misc_feature    671..691
                     /note="TEV site"
     misc_feature    692..1891
                     /note="BGT41_T01c3"
ORIGIN      
          1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
         61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
        121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
        181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
        241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
        301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
        361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
        421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
        481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
        541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC
        601 CTGTTCGTTG CAACAAATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA
        661 AGCAGGCTct gaaaacttgt actttcaagg cAAAGCTATT AATCCATGGG GATTACTTTC
        721 ATTAGAAAAT AATCCTTTAA ATCATTTAAA AAGATCTAAG AAATTTTATA AAGAAAAATA
        781 TATACGACCA ACTCAATATA TTAAACCTTC CCCAAAAAGT TTAATTCATA TAGGTTATTT
        841 CTCTGCTGAT TTCCTGAATC ATCCTGTAAT GCAACTAATT GCTCCTCTAC TTGAGCTACA
        901 TGATAAATAT AGGTTTAAAA TATATTTATA CTCATTTGTG CCAAAAGAAG ATGAATATAC
        961 TGAAAGAGCA AAAAAGTCTG GATGCATATT TAGAAACATC AAAAATTTAA ATGATATTGA
       1021 AGCAGTTGAA TTAGCAAGAA GTGATCAGTT AGATATTGCA GTAGATCTCA TGGGATATAC
       1081 CAGACACAAT AGAATGCCTA TATTCTCATA TAGGGTGGCA CCAATACAAA TCAATTACTT
       1141 AGGTTATAAT GGTTCTATTG GCTCAGATAC TATTGATTAT ATTATCGCGG ATAAAATCAC
       1201 AATTCCGAGA GAGTATGAAA AATTTTATTC CGAAAAAGTA ATACGATTGC CAAATTGCTT
       1261 TATATGTGAT GATAATAAAA AAGAAATTTC TAAAGAGTCC ATATCTCGTA AAGATTTCAA
       1321 CCTTCCTGAC CAAGGCTTTA TATTTACTTG TTTTAATAAT AATTATAAAA TAACAGAAAA
       1381 AGAATTTAAT ATATGGATGA ACTTACTTAG AAAAATAGAA GGAAGTGTTC TTTGGTTATA
       1441 TAAGGCAAAT CAATGGTCAA TGAATAATTT ATACAAGGAA GCGAGTAAAC GAAAAGTAGA
       1501 TCGAGACAGA ATAATATTCT CTGAAAAATT ACCAATGAGC AAACATCTAG CTAGGCATTC
       1561 TCTAGGTGAT TTAGCACTTG ATACTTTTAA TTACAATGGA GGTGTGACTA GTTCTAATGC
       1621 GTTGTGGACT GGTTTACCAG TGCTTACAAA AATAGGTCAG AATTTTACTG CTAGGATGTC
       1681 TGCCAGCCTG CTTACATCAT TGGGACTTCC CGAATTAATT ACTTATAGTG AAAGTGAATA
       1741 CGAAGATAAA GCTTTATATA TTGCTAGCAA CTCTGAAGAG ATTATTCGAT TGAAATCTAA
       1801 ATTAAACAAA TCGAAAGAAA CATCACCACT TTTTAATTCA AAATTATTTA CGCAAGATCT
       1861 TGAAAATATT TATCTTGATC TGGTAAAAAA ATAGGACCCA GCTTTCTTGT ACAAAGTTGG
       1921 CATTATAAGA AAGCATTGCT TATCAATTTG TTGCAACGAA CAGGTCACTA TCAGTCAAAA
       1981 TAAAATCATT ATTTGCCATC CAGCTGATAT CCCCTATAGT GAGTCGTATT ACATGGTCAT
       2041 AGCTGTTTCC TGGCAGCTCT GGCCCGTGTC TCAAAATCTC TGATGTTACA TTGCACAAGA
       2101 TAAAATAATA TCATCATGAA CAATAAAACT GTCTGCTTAC ATAAACAGTA ATACAAGGGG
       2161 TGTTATGAGC CATATTCAAC GGGAAACGTC GAGGCCGCGA TTAAATTCCA ACATGGATGC
       2221 TGATTTATAT GGGTATAAAT GGGCTCGCGA TAATGTCGGG CAATCAGGTG CGACAATCTA
       2281 TCGCTTGTAT GGGAAGCCCG ATGCGCCAGA GTTGTTTCTG AAACATGGCA AAGGTAGCGT
       2341 TGCCAATGAT GTTACAGATG AGATGGTCAG ACTAAACTGG CTGACGGAAT TTATGCCTCT
       2401 TCCGACCATC AAGCATTTTA TCCGTACTCC TGATGATGCA TGGTTACTCA CCACTGCGAT
       2461 CCCCGGAAAA ACAGCATTCC AGGTATTAGA AGAATATCCT GATTCAGGTG AAAATATTGT
       2521 TGATGCGCTG GCAGTGTTCC TGCGCCGGTT GCATTCGATT CCTGTTTGTA ATTGTCCTTT
       2581 TAACAGCGAT CGCGTATTTC GTCTCGCTCA GGCGCAATCA CGAATGAATA ACGGTTTGGT
       2641 TGATGCGAGT GATTTTGATG ACGAGCGTAA TGGCTGGCCT GTTGAACAAG TCTGGAAAGA
       2701 AATGCATAAA CTTTTGCCAT TCTCACCGGA TTCAGTCGTC ACTCATGGTG ATTTCTCACT
       2761 TGATAACCTT ATTTTTGACG AGGGGAAATT AATAGGTTGT ATTGATGTTG GACGAGTCGG
       2821 AATCGCAGAC CGATACCAGG ATCTTGCCAT CCTATGGAAC TGCCTCGGTG AGTTTTCTCC
       2881 TTCATTACAG AAACGGCTTT TTCAAAAATA TGGTATTGAT AATCCTGATA TGAATAAATT
       2941 GCAGTTTCAT TTGATGCTCG ATGAGTTTTT CTAATCAGAA TTGGTTAATT GGTTGTAACA
       3001 CTGGCAGAGC ATTACGCTGA CTTGACGGGA CGGCGCAAGC TCATGACCAA AATCCCTTAA
       3061 CGTGAGTTAC GCGTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC
       3121 TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC
       3181 AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT
       3241 CAGCAGAGCG CAGATACCAA ATACTGTTCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT
       3301 CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC
       3361 TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA
       3421 GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC
       3481 CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG
       3541 GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA
       3601 GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT
       3661 TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA
       3721 CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT T
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