LOCUS       BGT41_T01a2-pET32-DEST       7507 bp    DNA     circular     25-AUG-2011
FEATURES             Location/Qualifiers
     misc_feature    5208..5534
                     /note="Trx Tag"
     misc_feature    5556..5573
                     /note="His6-tag"
     misc_feature    7421..7482
                     /note="T7 Terminator"
     misc_feature    5139..5163
                     /note="Lac Operator"
     misc_feature    5120..5136
                     /note="T7 Promoter"
     misc_feature    5535..5552
                     /note="S Tag"
     misc_feature    5583..5600
                     /note="Thrombin"
     misc_feature    5667..5681
                     /note="Enterokinase"
     misc_recomb     complement(5693..5721)
                     /label=attB1
                     /note="attB1 site"
     misc_recomb     7275..7297
                     /label=attB2
                     /note="attB2 site"
     misc_feature    598..1458
                     /note="Ap"
     misc_feature    1613..2232
                     /note="Ori"
     misc_feature    complement(3647..4606)
                     /note="Lac I"
     misc_feature    5724..5744
                     /note="TEV site"
     misc_feature    5745..7268
                     /note="BGT41_T01a2"
     vector          join(7283..7507,1..5704)
                     /source="pET32-DEST"
                     /type="Destination Vector"
     clone           5705..7282
                     /source="BGT41_T01a2-pDONR221"
                     /type="Entry Clone"
ORIGIN      
          1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
         61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
        121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
        181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
        241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
        301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
        361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
        421 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt
        481 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
        541 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
        601 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
        661 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
        721 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
        781 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
        841 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
        901 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
        961 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
       1021 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
       1081 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
       1141 gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
       1201 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
       1261 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
       1321 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
       1381 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
       1441 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
       1501 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
       1561 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
       1621 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
       1681 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
       1741 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
       1801 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
       1861 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
       1921 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
       1981 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
       2041 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
       2101 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
       2161 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
       2221 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
       2281 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
       2341 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
       2401 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt
       2461 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
       2521 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
       2581 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
       2641 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc
       2701 atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt
       2761 gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt
       2821 tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat
       2881 gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg
       2941 gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa
       3001 aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag
       3061 ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt
       3121 ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga
       3181 cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc
       3241 agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac
       3301 ccgtggggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg
       3361 accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc
       3421 gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg
       3481 cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat
       3541 gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgaga
       3601 tcccggtgcc taatgagtga gctaacttac attaattgcg ttgcgctcac tgcccgcttt
       3661 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
       3721 cggtttgcgt attgggcgcc agggtggttt ttcttttcac cagtgagacg ggcaacagct
       3781 gattgccctt caccgcctgg ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc
       3841 ccagcaggcg aaaatcctgt ttgatggtgg ttaacggcgg gatataacat gagctgtctt
       3901 cggtatcgtc gtatcccact accgagatgt ccgcaccaac gcgcagcccg gactcggtaa
       3961 tggcgcgcat tgcgcccagc gccatctgat cgttggcaac cagcatcgca gtgggaacga
       4021 tgccctcatt cagcatttgc atggtttgtt gaaaaccgga catggcactc cagtcgcctt
       4081 cccgttccgc tatcggctga atttgattgc gagtgagata tttatgccag ccagccagac
       4141 gcagacgcgc cgagacagaa cttaatgggc ccgctaacag cgcgatttgc tggtgaccca
       4201 atgcgaccag atgctccacg cccagtcgcg taccgtcttc atgggagaaa ataatactgt
       4261 tgatgggtgt ctggtcagag acatcaagaa ataacgccgg aacattagtg caggcagctt
       4321 ccacagcaat ggcatcctgg tcatccagcg gatagttaat gatcagccca ctgacgcgtt
       4381 gcgcgagaag attgtgcacc gccgctttac aggcttcgac gccgcttcgt tctaccatcg
       4441 acaccaccac gctggcaccc agttgatcgg cgcgagattt aatcgccgcg acaatttgcg
       4501 acggcgcgtg cagggccaga ctggaggtgg caacgccaat cagcaacgac tgtttgcccg
       4561 ccagttgttg tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc gcttccactt
       4621 tttcccgcgt tttcgcagaa acgtggctgg cctggttcac cacgcgggaa acggtctgat
       4681 aagagacacc ggcatactct gcgacatcgt ataacgttac tggtttcaca ttcaccaccc
       4741 tgaattgact ctcttccggg cgctatcatg ccataccgcg aaaggttttg cgccattcga
       4801 tggtgtccgg gatctcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt
       4861 agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg
       4921 cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg
       4981 agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca
       5041 accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc
       5101 gatctcgatc ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat
       5161 tcccctctag aaataatttt gtttaacttt aagaaggaga tatacatatg agcgataaaa
       5221 ttattcacct gactgacgac agttttgaca cggatgtact caaagcggac ggggcgatcc
       5281 tcgtcgattt ctgggcagag tggtgcggtc cgtgcaaaat gatcgccccg attctggatg
       5341 aaatcgctga cgaatatcag ggcaaactga ccgttgcaaa actgaacatc gatcaaaacc
       5401 ctggcactgc gccgaaatat ggcatccgtg gtatcccgac tctgctgctg ttcaaaaacg
       5461 gtgaagtggc ggcaaccaaa gtgggtgcac tgtctaaagg tcagttgaaa gagttcctcg
       5521 acgctaacct ggccggttct ggttctggcc atatgcacca tcatcatcat cattcttctg
       5581 gtctggtgcc acgcggttct ggtatgaaag aaaccgctgc tgctaaattc gaacgccagc
       5641 acatggacag cccagatctg ggtaccgacg acgacgacaa ggccatggcg atATCAACAA
       5701 GTTTGTACAA AAAAGCAGGC Tctgaaaact tgtactttca aggcACCGAA GAAAGAAAGA
       5761 ATCAAAAGCA AGAAGGCTCT AAAGTAAAAA CATTCCCAGT CTCTTTTGCT TTAGGTGAAA
       5821 TTAAAGAAAA CATCACTATT ACGACTAATA GCGCTTCTAA TACTTCAAAG GAACAAAAAA
       5881 GATTTGGAGA CCAGAGTAAA ACTATAAAGA AAAAAGATAC CAATACAATT ACTAAACCTT
       5941 CCAAAGATCA AATCATTAAT CAAGCATTTA AATTTCATTC ACAAGGCAAT ATAAAAGAAG
       6001 CAGCAAAAAA TTATCAATAT TTCATTAATC AAGGTTTCTC TGACCACATG GTTTTTTCTA
       6061 ATTATGGAGC AATATTAAGA GATCTTGGTA ATTTACAAGA TGCAGAATTA TATACTCGCA
       6121 AAGCAATTAA AATTAATCCT AATTACGCAT TGGCTTACTC TAATCTGGGA AATGTATTAA
       6181 AAGATCTTGG TAAGTCACAA GATGCAGAAT TGTCATACCG CAAAGCAATT CAAATTAATC
       6241 CTAATTACGC AGATGCACAT TACAATCTGG GAATAATATT GAAAGAACTT GGTAATTTAC
       6301 AAGACGCAGA ATTGTCATAT CGCAAAGCAA TTCAAATTAA TCCTAATTAC GCAGATGCAT
       6361 ATTCCAATCT GGGAAATGTA TTAAAAGATC TTGATAATTT ACAAGACGCA GAATTGTCAT
       6421 ACCGCAAAGC AATTCAAATT AATCCTAGTT ACGCAGACGC ATATTCAAAT CTGGGAAATG
       6481 TATTAAAAGA TCTTGGTAAT TTACAAGACG CAGAGTTGTC ATACCGCAAA GCAATTCAAA
       6541 TTAATCCTGA TTACGCAGAG GCGCATTTCA ATCTAGGAAA TCTATTAAAA GATCTTGGTA
       6601 AATTACAAGA CGCAGAGTTG TCATATCGCA AAGCAATTCA AATCAAATCT GATTACGCTG
       6661 AGGCGCATTA CAATCTAGGA ATCATATTGA AAGACCTTGG TAATTTACAA GACGCAGAAT
       6721 TTTATAATCG CAAAGCAATT CAAATCAAAC CTGATTACGC TGAGGCGCAT TTCAATCTGG
       6781 GAATCATATT GAAAGACCTT GGTAATTTAC AAGACGCAGA ATTCTCATAT CGCCAAGCAA
       6841 TTCAAATCAA ACCTGATTAC GCAGATGCCT ACTCCAATCT GGGAAATGTA TTAAAAGATC
       6901 TTGGTAAGTT AAAAGACGCA GAATTGTCAT ATCGCAAAGC AATTCAAATC AAACCTGATT
       6961 ACGCTGAGGT TTATTCCAAT CTGGGAAATG TATTAAAAGA TCTTGGTAAT TTACAAGACG
       7021 CAGAATTTTC ATACCGCAAA GCAATTCAAA TCAAACCTGA TTACGCAGAT GCTTACTCCA
       7081 ATCTGGGAAA TATATTAAAA GAGCTAAGTA ATTTCACTGA CGCTATAAAT CAATTCAAGG
       7141 ATGCACTAAA ATTGAACAAT GAATTAACAT CAGCTCAGAC TGGTTTAATG TCAACTCAGG
       7201 GTAATATATG CGATTGGAGT GATGAGGAGA CTCATAATAA ATGGCTTAAA TCACTTGGTA
       7261 TTAAAGGATA GGACCCAGCT TTCTTGTACA AAGTGGTTGA Tatcggatcc gaattcgagc
       7321 tccgtcgaca agcttgcggc cgcactcgag caccaccacc accaccactg agatccggct
       7381 gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca
       7441 taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata
       7501 tccggat
//
