LOCUS       BGT41_T01a2-pET16-DEST       7324 bp    DNA     circular     25-AUG-2011
FEATURES             Location/Qualifiers
     misc_feature    5323..5352
                     /note="His10-tag"
     misc_feature    5365..5376
                     /note="X-factor"
     misc_feature    5230..5245
                     /note="T7 Promoter"
     misc_feature    5249..5273
                     /note="Lac O"
     misc_feature    7051..7112
                     /note="T7 Terminator"
     misc_recomb     complement(5392..5416)
                     /label=attB1
                     /note="attB1 site"
     misc_recomb     6970..6992
                     /label=attB2
                     /note="attB2 site"
     misc_feature    complement(3761..4720)
                     /note="Lac I"
     misc_feature    209..1069
                     /note="Ap"
     misc_feature    1224..1843
                     /note="Ori"
     misc_feature    5419..5439
                     /note="TEV site"
     misc_feature    5440..6963
                     /note="BGT41_T01a2"
     vector          join(6978..7324,1..5399)
                     /source="pET16-DEST"
                     /type="Destination Vector"
     clone           5400..6977
                     /source="BGT41_T01a2-pDONR221"
                     /type="Entry Clone"
ORIGIN      
          1 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
         61 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
        121 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
        181 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
        241 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
        301 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
        361 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
        421 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg
        481 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
        541 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
        601 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
        661 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
        721 accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact
        781 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
        841 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
        901 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
        961 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
       1021 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
       1081 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
       1141 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
       1201 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
       1261 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
       1321 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
       1381 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
       1441 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
       1501 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
       1561 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
       1621 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
       1681 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
       1741 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
       1801 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
       1861 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
       1921 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
       1981 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
       2041 tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc
       2101 gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc
       2161 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
       2221 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
       2281 cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga
       2341 tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc
       2401 ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg
       2461 tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca
       2521 cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac
       2581 tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg
       2641 ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga
       2701 acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga
       2761 agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc
       2821 gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg
       2881 tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga
       2941 tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg
       3001 tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc
       3061 cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg
       3121 acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt
       3181 ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt
       3241 taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg
       3301 cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat
       3361 aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc
       3421 ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt
       3481 gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat
       3541 cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg
       3601 tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg
       3661 cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gagatcccgg
       3721 tgcctaatga gtgagctaac ttacattaat tgcgttgcgc tcactgcccg ctttccagtc
       3781 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
       3841 gcgtattggg cgccagggtg gtttttcttt tcaccagtga gacgggcaac agctgattgc
       3901 ccttcaccgc ctggccctga gagagttgca gcaagcggtc cacgctggtt tgccccagca
       3961 ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata acatgagctg tcttcggtat
       4021 cgtcgtatcc cactaccgag atatccgcac caacgcgcag cccggactcg gtaatggcgc
       4081 gcattgcgcc cagcgccatc tgatcgttgg caaccagcat cgcagtggga acgatgccct
       4141 cattcagcat ttgcatggtt tgttgaaaac cggacatggc actccagtcg ccttcccgtt
       4201 ccgctatcgg ctgaatttga ttgcgagtga gatatttatg ccagccagcc agacgcagac
       4261 gcgccgagac agaacttaat gggcccgcta acagcgcgat ttgctggtga cccaatgcga
       4321 ccagatgctc cacgcccagt cgcgtaccgt cttcatggga gaaaataata ctgttgatgg
       4381 gtgtctggtc agagacatca agaaataacg ccggaacatt agtgcaggca gcttccacag
       4441 caatggcatc ctggtcatcc agcggatagt taatgatcag cccactgacg cgttgcgcga
       4501 gaagattgtg caccgccgct ttacaggctt cgacgccgct tcgttctacc atcgacacca
       4561 ccacgctggc acccagttga tcggcgcgag atttaatcgc cgcgacaatt tgcgacggcg
       4621 cgtgcagggc cagactggag gtggcaacgc caatcagcaa cgactgtttg cccgccagtt
       4681 gttgtgccac gcggttggga atgtaattca gctccgccat cgccgcttcc actttttccc
       4741 gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc tgataagaga
       4801 caccggcata ctctgcgaca tcgtataacg ttactggttt cacattcacc accctgaatt
       4861 gactctcttc cgggcgctat catgccatac cgcgaaaggt tttgcgccat tcgatggtgt
       4921 ccgggatctc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg
       4981 ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac
       5041 agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg
       5101 aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca
       5161 cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc
       5221 ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag
       5281 aaataatttt gtttaacttt aagaaggaga tataccatgg gccatcatca tcatcatcat
       5341 catcatcatc acagcagcgg ccatatcgaa ggtcgtcata tgctcgaATC AACAAGTTTG
       5401 TACAAAAAAG CAGGCTctga aaacttgtac tttcaaggcA CCGAAGAAAG AAAGAATCAA
       5461 AAGCAAGAAG GCTCTAAAGT AAAAACATTC CCAGTCTCTT TTGCTTTAGG TGAAATTAAA
       5521 GAAAACATCA CTATTACGAC TAATAGCGCT TCTAATACTT CAAAGGAACA AAAAAGATTT
       5581 GGAGACCAGA GTAAAACTAT AAAGAAAAAA GATACCAATA CAATTACTAA ACCTTCCAAA
       5641 GATCAAATCA TTAATCAAGC ATTTAAATTT CATTCACAAG GCAATATAAA AGAAGCAGCA
       5701 AAAAATTATC AATATTTCAT TAATCAAGGT TTCTCTGACC ACATGGTTTT TTCTAATTAT
       5761 GGAGCAATAT TAAGAGATCT TGGTAATTTA CAAGATGCAG AATTATATAC TCGCAAAGCA
       5821 ATTAAAATTA ATCCTAATTA CGCATTGGCT TACTCTAATC TGGGAAATGT ATTAAAAGAT
       5881 CTTGGTAAGT CACAAGATGC AGAATTGTCA TACCGCAAAG CAATTCAAAT TAATCCTAAT
       5941 TACGCAGATG CACATTACAA TCTGGGAATA ATATTGAAAG AACTTGGTAA TTTACAAGAC
       6001 GCAGAATTGT CATATCGCAA AGCAATTCAA ATTAATCCTA ATTACGCAGA TGCATATTCC
       6061 AATCTGGGAA ATGTATTAAA AGATCTTGAT AATTTACAAG ACGCAGAATT GTCATACCGC
       6121 AAAGCAATTC AAATTAATCC TAGTTACGCA GACGCATATT CAAATCTGGG AAATGTATTA
       6181 AAAGATCTTG GTAATTTACA AGACGCAGAG TTGTCATACC GCAAAGCAAT TCAAATTAAT
       6241 CCTGATTACG CAGAGGCGCA TTTCAATCTA GGAAATCTAT TAAAAGATCT TGGTAAATTA
       6301 CAAGACGCAG AGTTGTCATA TCGCAAAGCA ATTCAAATCA AATCTGATTA CGCTGAGGCG
       6361 CATTACAATC TAGGAATCAT ATTGAAAGAC CTTGGTAATT TACAAGACGC AGAATTTTAT
       6421 AATCGCAAAG CAATTCAAAT CAAACCTGAT TACGCTGAGG CGCATTTCAA TCTGGGAATC
       6481 ATATTGAAAG ACCTTGGTAA TTTACAAGAC GCAGAATTCT CATATCGCCA AGCAATTCAA
       6541 ATCAAACCTG ATTACGCAGA TGCCTACTCC AATCTGGGAA ATGTATTAAA AGATCTTGGT
       6601 AAGTTAAAAG ACGCAGAATT GTCATATCGC AAAGCAATTC AAATCAAACC TGATTACGCT
       6661 GAGGTTTATT CCAATCTGGG AAATGTATTA AAAGATCTTG GTAATTTACA AGACGCAGAA
       6721 TTTTCATACC GCAAAGCAAT TCAAATCAAA CCTGATTACG CAGATGCTTA CTCCAATCTG
       6781 GGAAATATAT TAAAAGAGCT AAGTAATTTC ACTGACGCTA TAAATCAATT CAAGGATGCA
       6841 CTAAAATTGA ACAATGAATT AACATCAGCT CAGACTGGTT TAATGTCAAC TCAGGGTAAT
       6901 ATATGCGATT GGAGTGATGA GGAGACTCAT AATAAATGGC TTAAATCACT TGGTATTAAA
       6961 GGATAGGACC CAGCTTTCTT GTACAAAGTG GTTGATtcga ggatccggct gctaacaaag
       7021 cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg
       7081 gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc
       7141 ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac
       7201 ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta
       7261 gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg
       7321 agaa
//
