LOCUS       pDONR/Zeo
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FEATURES             Location/Qualifiers
     misc_feature    complement(268..295)
                     /note="rrnB T2 transcription termination sequence (c)"
     misc_feature    complement(427..470)
                     /note="rrnB T1 transcription termination sequence (c)"
     primer_bind     537..552
                     /note="M13 Forward (-20) priming site"
     misc_recomb     570..668
                     /label=attL1
                     /note="attL1"
     misc_recomb     complement(1316..1412)
                     /label=attL2
                     /note="attL2 (c)"
     misc_signal     complement(1427..1446)
                     /note="T7 Promoter/priming site (c)"
     primer_bind     1454..1470
                     /note="M13 Reverse priming site"
     gene            1583..2392
                     /note="Kanamycin resistance gene"
     rep_origin      2513..3186
                     /note="pUC origin"
     vector          join(1324..3189,1..651)
                     /source="pDONR%99221"
                     /type="Donor Vector"
     misc_feature    671..691
                     /note="TEV site"
     misc_feature    692..1021
                     /note="Uknown"
     misc_feature    1022..1309
                     /note="AamyC beta sheets"
     misc_feature    692..1309
                     /note="BGH13_B05a3"
ORIGIN      
          1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
         61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
        121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
        181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
        241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
        301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
        361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
        421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
        481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
        541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC
        601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA
        661 AGCAGGCTct gaaaacttgt actttcaagg cCTGATTGAT GCCGATATGT ATTGGCATAT
        721 GCAGAAAGGC GATGAGAATT ATGTAGTCAA TCGCGGAATC GCTTTACATA AGATGATCCG
        781 CTTGCTGACA TCTTCGACGA TCAATGGCGG TTATCTGAAC TTTATGGGTA ATGAGTTCGG
        841 GCATCCCGAA TGGATCGATT TTCCGCGTGA AGGTAATGGA TGGTCTTGTA AGTATGCCCG
        901 TCGTCAGTGG GATCTGGTAG ATAACAAGAA TCTGGCTTAT CACTACATGG GGGACTTTGA
        961 TGCTGAGATG CTGAAAGTGA TCAAGAGTGT GAAAGATTTC CAGGCGACTC CGGTCCAGGA
       1021 GATCTGGCAT AATGATGGTG ATCAGGTATT GGCTTATGGA CGTAAGGATC TGATATTTGT
       1081 ATTTAACTTC AATCCGAAAC AGTCTTTTGT AGATTATGGT TTCCTCGTTA GTCCGGGAGC
       1141 TTATGAAGTA ATCTTGAATA CGGATAATGT TGCATTTGGC GGAAATGGTC TTGCAGATGA
       1201 TTCAGTAGTT CATTTCACGA TTGCCGATCC GTTGTATAAG AAGGAAAAGA AAGAGTGGCT
       1261 GAAACTCTAT ATTCCTGCAC GTACAGCTGT TGTTTTGAGA AAGAAGAAAT AGGACCCAGC
       1321 TTTCTTGTAC AAAGTTGGCA TTATAAGAAA GCATTGCTTA TCAATTTGTT GCAACGAACA
       1381 GGTCACTATC AGTCAAAATA AAATCATTAT TTGCCATCCA GCTGATATCC CCTATAGTGA
       1441 GTCGTATTAC ATGGTCATAG CTGTTTCCTG GCAGCTCTGG CCCGTGTCTC AAAATCTCTG
       1501 ATGTTACATT GCACAAGATA AAATAATATC ATCATGAACA ATAAAACTGT CTGCTTACAT
       1561 AAACAGTAAT ACAAGGGGTG TTATGAGCCA TATTCAACGG GAAACGTCGA GGCCGCGATT
       1621 AAATTCCAAC ATGGATGCTG ATTTATATGG GTATAAATGG GCTCGCGATA ATGTCGGGCA
       1681 ATCAGGTGCG ACAATCTATC GCTTGTATGG GAAGCCCGAT GCGCCAGAGT TGTTTCTGAA
       1741 ACATGGCAAA GGTAGCGTTG CCAATGATGT TACAGATGAG ATGGTCAGAC TAAACTGGCT
       1801 GACGGAATTT ATGCCTCTTC CGACCATCAA GCATTTTATC CGTACTCCTG ATGATGCATG
       1861 GTTACTCACC ACTGCGATCC CCGGAAAAAC AGCATTCCAG GTATTAGAAG AATATCCTGA
       1921 TTCAGGTGAA AATATTGTTG ATGCGCTGGC AGTGTTCCTG CGCCGGTTGC ATTCGATTCC
       1981 TGTTTGTAAT TGTCCTTTTA ACAGCGATCG CGTATTTCGT CTCGCTCAGG CGCAATCACG
       2041 AATGAATAAC GGTTTGGTTG ATGCGAGTGA TTTTGATGAC GAGCGTAATG GCTGGCCTGT
       2101 TGAACAAGTC TGGAAAGAAA TGCATAAACT TTTGCCATTC TCACCGGATT CAGTCGTCAC
       2161 TCATGGTGAT TTCTCACTTG ATAACCTTAT TTTTGACGAG GGGAAATTAA TAGGTTGTAT
       2221 TGATGTTGGA CGAGTCGGAA TCGCAGACCG ATACCAGGAT CTTGCCATCC TATGGAACTG
       2281 CCTCGGTGAG TTTTCTCCTT CATTACAGAA ACGGCTTTTT CAAAAATATG GTATTGATAA
       2341 TCCTGATATG AATAAATTGC AGTTTCATTT GATGCTCGAT GAGTTTTTCT AATCAGAATT
       2401 GGTTAATTGG TTGTAACACT GGCAGAGCAT TACGCTGACT TGACGGGACG GCGCAAGCTC
       2461 ATGACCAAAA TCCCTTAACG TGAGTTACGC GTCGTTCCAC TGAGCGTCAG ACCCCGTAGA
       2521 AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC
       2581 AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT
       2641 TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTTCTTC TAGTGTAGCC
       2701 GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT
       2761 CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG
       2821 ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC
       2881 CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG
       2941 CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC
       3001 AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG
       3061 GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT
       3121 ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC
       3181 TCACATGTT
//
