LOCUS       pDONR/Zeo
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FEATURES             Location/Qualifiers
     misc_feature    complement(268..295)
                     /note="rrnB T2 transcription termination sequence (c)"
     misc_feature    complement(427..470)
                     /note="rrnB T1 transcription termination sequence (c)"
     primer_bind     537..552
                     /note="M13 Forward (-20) priming site"
     misc_recomb     570..668
                     /label=attL1
                     /note="attL1"
     misc_recomb     complement(2021..2117)
                     /label=attL2
                     /note="attL2 (c)"
     misc_signal     complement(2132..2151)
                     /note="T7 Promoter/priming site (c)"
     primer_bind     2159..2175
                     /note="M13 Reverse priming site"
     gene            2288..3097
                     /note="Kanamycin resistance gene"
     rep_origin      3218..3891
                     /note="pUC origin"
     vector          join(2029..3894,1..651)
                     /source="pDONR%99221"
                     /type="Donor Vector"
     misc_feature    671..691
                     /note="TEV site"
     misc_feature    692..2014
                     /note="BGH13_B01b2"
ORIGIN      
          1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
         61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
        121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
        181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
        241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
        301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
        361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
        421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
        481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
        541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC
        601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA
        661 AGCAGGCTct gaaaacttgt actttcaagg cCAACCGCGA ACCGCGTTCG TTCATTTGTT
        721 CGAATGGCAG TGGAACGATA TTGCTAGCGA GTGCGAAAAT GTACTGGGGC CAAAAGGCTA
        781 TGCGGCAGTG CAGGTATCCC CACCCCAAAA ATCGGTTAGT AGCAGCCAAT GGTGGAGTCG
        841 TTACCAACCT GTTAGCTATG CCATAGAGGG CCGTTCTGGC AATCGCAACG AATTCGCCAG
        901 CATGGTAAGC CGGTGTAAAA ATGCTGGGGT GGATATATAC GTAGATGCCG TAATTAATCA
        961 TATGGCTGCC GGCGGGCGCT ATTACCCAGA AGTGCCTTAC TACGCAGAGC ATTTTCACAC
       1021 GTGCTACGGC CAAATTGATT ACGGTAACCG CTGGCAGGTA CAAAACTGTG ACTTGGTAGG
       1081 GCTAAACGAT TTAGCAACAG AACAAGACTA TGTACGCACA AAAATAGCCA ATTACTTAAA
       1141 CGACTTAACT AACCTTGGCG TAGCTGGCTT CCGCTTTGAT GCTGCGAAAC ATATCCCCGT
       1201 GGGTGACATT GCTGCAATTA AAGCAAAACT TATTGGCAAC CCGTATATAT TCCAAGAAGT
       1261 GATAGGTGCG CCGGGCGAGC CCATCCAACC ATCAGAATAT ACCTACTTAG CAGACGTAAC
       1321 AGAGTTTAAC TTTACCAAAT CGGTTAGCCA TTTTTTTAAA GGGCGCGGAC CAATTAAAGA
       1381 GTTAAAAAAT ATTGGTACTT GGGGCGGTTG GGTTGCAAGT GCAGACGCCG TAACCTTTGT
       1441 TGCCAACCAC GATAACCAGC GCCAGGATAC AAACAACACC ATTACCCATA AAGATGGTGA
       1501 TAACCGCAAT ACTATGGCGC ACGTATTTAT GCTGGGCTGG CCCTATGGGT ACCCTAAAGT
       1561 AATGTCTAGT TATGAGTGGT ACGATCACGA CCAAGGCCCT CCCGCCCACG GTGCTAGCAG
       1621 CTGTAACAGC GGCTGGTTAT GTGAACACCG CACGCCAGCT ATCGCCAATA TGGTTGCTTT
       1681 TCGCAATTTC ACTGCAGACG AATTTCGCGT AACCAACTAT TGGGATAACG GCAACAATCA
       1741 AATAGCTTGG GGCCGAGGCG GCAAAGGGTT TGTAGTTATA AATATGGAAG GCAACAATTT
       1801 AACCCGTACA TTTCAAACAG GGATGCCCGC TGGCCGTTAT TGCGACATTA TTTCGGGTAA
       1861 CTACAACGCG TCTAATAAAA GTTGTTCTGG CAATATTATA GATGTAGATA ACAATGGCAA
       1921 CGCAGTAATC ACCGCTTACT ACCGCAACGG TGCCGCAATT CATGTAGGCG CTATTGTTGA
       1981 AGACTGTGAC GGTACAGATT GCTGCACAAA CTGCTAGGAC CCAGCTTTCT TGTACAAAGT
       2041 TGGCATTATA AGAAAGCATT GCTTATCAAT TTGTTGCAAC GAACAGGTCA CTATCAGTCA
       2101 AAATAAAATC ATTATTTGCC ATCCAGCTGA TATCCCCTAT AGTGAGTCGT ATTACATGGT
       2161 CATAGCTGTT TCCTGGCAGC TCTGGCCCGT GTCTCAAAAT CTCTGATGTT ACATTGCACA
       2221 AGATAAAATA ATATCATCAT GAACAATAAA ACTGTCTGCT TACATAAACA GTAATACAAG
       2281 GGGTGTTATG AGCCATATTC AACGGGAAAC GTCGAGGCCG CGATTAAATT CCAACATGGA
       2341 TGCTGATTTA TATGGGTATA AATGGGCTCG CGATAATGTC GGGCAATCAG GTGCGACAAT
       2401 CTATCGCTTG TATGGGAAGC CCGATGCGCC AGAGTTGTTT CTGAAACATG GCAAAGGTAG
       2461 CGTTGCCAAT GATGTTACAG ATGAGATGGT CAGACTAAAC TGGCTGACGG AATTTATGCC
       2521 TCTTCCGACC ATCAAGCATT TTATCCGTAC TCCTGATGAT GCATGGTTAC TCACCACTGC
       2581 GATCCCCGGA AAAACAGCAT TCCAGGTATT AGAAGAATAT CCTGATTCAG GTGAAAATAT
       2641 TGTTGATGCG CTGGCAGTGT TCCTGCGCCG GTTGCATTCG ATTCCTGTTT GTAATTGTCC
       2701 TTTTAACAGC GATCGCGTAT TTCGTCTCGC TCAGGCGCAA TCACGAATGA ATAACGGTTT
       2761 GGTTGATGCG AGTGATTTTG ATGACGAGCG TAATGGCTGG CCTGTTGAAC AAGTCTGGAA
       2821 AGAAATGCAT AAACTTTTGC CATTCTCACC GGATTCAGTC GTCACTCATG GTGATTTCTC
       2881 ACTTGATAAC CTTATTTTTG ACGAGGGGAA ATTAATAGGT TGTATTGATG TTGGACGAGT
       2941 CGGAATCGCA GACCGATACC AGGATCTTGC CATCCTATGG AACTGCCTCG GTGAGTTTTC
       3001 TCCTTCATTA CAGAAACGGC TTTTTCAAAA ATATGGTATT GATAATCCTG ATATGAATAA
       3061 ATTGCAGTTT CATTTGATGC TCGATGAGTT TTTCTAATCA GAATTGGTTA ATTGGTTGTA
       3121 ACACTGGCAG AGCATTACGC TGACTTGACG GGACGGCGCA AGCTCATGAC CAAAATCCCT
       3181 TAACGTGAGT TACGCGTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC
       3241 TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT
       3301 ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG
       3361 CTTCAGCAGA GCGCAGATAC CAAATACTGT TCTTCTAGTG TAGCCGTAGT TAGGCCACCA
       3421 CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC
       3481 TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA
       3541 TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC
       3601 GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA
       3661 AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG
       3721 GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG
       3781 ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG
       3841 CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTT
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