LOCUS       pDONR/Zeo
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FEATURES             Location/Qualifiers
     misc_feature    complement(268..295)
                     /note="rrnB T2 transcription termination sequence (c)"
     misc_feature    complement(427..470)
                     /note="rrnB T1 transcription termination sequence (c)"
     primer_bind     537..552
                     /note="M13 Forward (-20) priming site"
     misc_recomb     570..668
                     /label=attL1
                     /note="attL1"
     misc_recomb     complement(1784..1880)
                     /label=attL2
                     /note="attL2 (c)"
     misc_signal     complement(1895..1914)
                     /note="T7 Promoter/priming site (c)"
     primer_bind     1922..1938
                     /note="M13 Reverse priming site"
     gene            2051..2860
                     /note="Kanamycin resistance gene"
     rep_origin      2981..3654
                     /note="pUC origin"
     vector          join(1792..3657,1..651)
                     /source="pDONR%99221"
                     /type="Donor Vector"
     misc_feature    669..691
                     /note="TEV site"
     source          692..1777
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="MGC:1271 IMAGE:2988041"
                     /tissue_type="Colon, adenocarcinoma"
                     /clone_lib="NIH_MGC_15"
                     /lab_host="DH10B-R"
                     /note="Vector: pOTB7"
     gene            692..1777
                     /gene="B3GNT1"
                     /gene_synonym=B3GN-T1
                     /gene_synonym=BETA3GNTI
                     /gene_synonym=iGAT
                     /gene_synonym=iGNT
                     /db_xref="GeneID:11041"
                     /db_xref="HGNC:15685"
                     /db_xref="MIM:605517"
     CDS             692..1777
                     /dnas_title="UDP-GlcNAc:betaGal
                     beta-1,3-N-acetylglucosaminyltransferase 1"
                     /gene="B3GNT1"
                     /gene_synonym=B3GN-T1
                     /gene_synonym=BETA3GNTI
                     /gene_synonym=iGAT
                     /gene_synonym=iGNT
                     /codon_start=1
                     /product="UDP-GlcNAc:betaGal
                     beta-1,3-N-acetylglucosaminyltransferase 1"
                     /protein_id="AAH21965.1"
                     /db_xref="GI:18314366"
                     /db_xref="GeneID:11041"
                     /db_xref="HGNC:15685"
                     /db_xref="MIM:605517"
                     /translation="MQMSYAIRCAFYQLLLAALMLVAMLQLLYLSLLSGLHGQEEQDQ
                     YFEFFPPSPRSVDQVKAQLRTALASGGVLDASGDYRVYRGLLKTTMDPNDVILATHAS
                     VDNLLHLSGLLERWEGPLSVSVFAATKEEAQLATVLAYALSSHCPDMRARVAMHLVCP
                     SRYEAAVPDPREPGEFALLRSCQEVFDKLARVAQPGINYALGTNVSYPNNLLRNLARE
                     GANYALVIDVDMVPSEGLWRGLREMLDQSNQWGGTALVVPAFEIRRARRMPMNKNELV
                     QLYQVGEVRPFYYGLCTPCQAPTNYSRWVNLPEESLLRPAYVVPWQDPWEPFYVAGGK
                     VPTFDERFRQYGFNRISQACELHVAGFDFEVLNEGFLVHKGFKEALKFHPQKEAENQH
                     NKILYRQFKQELKAKYPNSPRRC"
     misc_feature    692..1777
                     /note="B3GNT1 coding region"
ORIGIN      
          1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
         61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
        121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
        181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
        241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
        301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
        361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
        421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
        481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
        541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC
        601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA
        661 AGCAGGCTct gaaaacttgt actttcaagg ccggtccgtg gaccaggtca aggcgcagct
        721 ccgcaccgcg ctggcctctg gaggcgtcct ggacgctagc ggcgattacc gcgtctacag
        781 gggcctgctg aagaccacca tggaccccaa cgatgtgatc ctggccacgc acgccagcgt
        841 ggacaacctg ctgcacctgt cgggtctgct ggagcgctgg gagggcccgc tgtccgtgtc
        901 ggtgttcgcg gccaccaagg aggaggcgca gctggccacg gtgctggcct acgcgctgag
        961 cagccactgc cccgacatgc gcgccagggt cgccatgcac ctcgtgtgcc cctcgcgtta
       1021 cgaggcagcc gtgcccgacc cccgggagcc gggggagttt gccctgctgc ggtcctgcca
       1081 ggaggtcttt gacaagctag ccagggtggc ccagcccggg attaattatg cgctgggcac
       1141 caatgtctcc taccccaata acctgctgag gaatctggct cgtgaggggg ccaactatgc
       1201 cctggtgatc gatgtggaca tggtgcccag cgaggggctg tggagaggcc tgcgggaaat
       1261 gctggatcag agcaaccagt ggggaggcac cgcgctggtg gtgcctgcct tcgaaatccg
       1321 aagagcccgc cgcatgccca tgaacaaaaa cgagctggtg cagctctacc aggttggcga
       1381 ggtgcggccc ttctattatg ggttgtgcac cccctgccag gcacccacca actattcccg
       1441 ctgggtcaac ctgccggaag agagcttgct gcggcccgcc tacgtggtac cttggcagga
       1501 cccctgggag ccattctacg tggcaggagg caaggtgccc accttcgacg agcgctttcg
       1561 gcagtacggc ttcaaccgaa tcagccaggc ctgcgagctg catgtggcgg ggtttgattt
       1621 tgaggtcctg aacgaaggtt tcttggttca taagggcttc aaagaagcgt tgaagttcca
       1681 tccccaaaag gaggctgaaa atcagcacaa taagatccta tatcgccagt tcaaacagga
       1741 gttgaaggcc aagtacccca actctccccg acgctgcTAG GACCCAGCTT TCTTGTACAA
       1801 AGTTGGCATT ATAAGAAAGC ATTGCTTATC AATTTGTTGC AACGAACAGG TCACTATCAG
       1861 TCAAAATAAA ATCATTATTT GCCATCCAGC TGATATCCCC TATAGTGAGT CGTATTACAT
       1921 GGTCATAGCT GTTTCCTGGC AGCTCTGGCC CGTGTCTCAA AATCTCTGAT GTTACATTGC
       1981 ACAAGATAAA ATAATATCAT CATGAACAAT AAAACTGTCT GCTTACATAA ACAGTAATAC
       2041 AAGGGGTGTT ATGAGCCATA TTCAACGGGA AACGTCGAGG CCGCGATTAA ATTCCAACAT
       2101 GGATGCTGAT TTATATGGGT ATAAATGGGC TCGCGATAAT GTCGGGCAAT CAGGTGCGAC
       2161 AATCTATCGC TTGTATGGGA AGCCCGATGC GCCAGAGTTG TTTCTGAAAC ATGGCAAAGG
       2221 TAGCGTTGCC AATGATGTTA CAGATGAGAT GGTCAGACTA AACTGGCTGA CGGAATTTAT
       2281 GCCTCTTCCG ACCATCAAGC ATTTTATCCG TACTCCTGAT GATGCATGGT TACTCACCAC
       2341 TGCGATCCCC GGAAAAACAG CATTCCAGGT ATTAGAAGAA TATCCTGATT CAGGTGAAAA
       2401 TATTGTTGAT GCGCTGGCAG TGTTCCTGCG CCGGTTGCAT TCGATTCCTG TTTGTAATTG
       2461 TCCTTTTAAC AGCGATCGCG TATTTCGTCT CGCTCAGGCG CAATCACGAA TGAATAACGG
       2521 TTTGGTTGAT GCGAGTGATT TTGATGACGA GCGTAATGGC TGGCCTGTTG AACAAGTCTG
       2581 GAAAGAAATG CATAAACTTT TGCCATTCTC ACCGGATTCA GTCGTCACTC ATGGTGATTT
       2641 CTCACTTGAT AACCTTATTT TTGACGAGGG GAAATTAATA GGTTGTATTG ATGTTGGACG
       2701 AGTCGGAATC GCAGACCGAT ACCAGGATCT TGCCATCCTA TGGAACTGCC TCGGTGAGTT
       2761 TTCTCCTTCA TTACAGAAAC GGCTTTTTCA AAAATATGGT ATTGATAATC CTGATATGAA
       2821 TAAATTGCAG TTTCATTTGA TGCTCGATGA GTTTTTCTAA TCAGAATTGG TTAATTGGTT
       2881 GTAACACTGG CAGAGCATTA CGCTGACTTG ACGGGACGGC GCAAGCTCAT GACCAAAATC
       2941 CCTTAACGTG AGTTACGCGT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG
       3001 ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC
       3061 GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC
       3121 TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTTCTTCTA GTGTAGCCGT AGTTAGGCCA
       3181 CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT
       3241 GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC
       3301 GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG
       3361 AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC
       3421 CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC
       3481 GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT
       3541 CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC
       3601 CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTT
//
