LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2927..3023) /label=attL2 /note="attL2 (c)" misc_signal complement(3038..3057) /note="T7 Promoter/priming site (c)" primer_bind 3065..3081 /note="M13 Reverse priming site" gene 3194..4003 /note="Kanamycin resistance gene" rep_origin 4124..4797 /note="pUC origin" vector join(2935..4800,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..2920 /organism="synthetic construct" /mol_type="other DNA" /db_xref="taxon:32630" /clone="MGC:190543 IMAGE:100062526" /clone_lib="NIH_MGC_428" /lab_host="DH10B" /focus /note="Vector: pENTR223.1 with stop codon" source 692..2920 /organism="Homo sapiens" /mol_type="other DNA" /db_xref="taxon:9606" gene 692..2920 /gene="MGAT5B" /gene_synonym=GnT-IX /gene_synonym=GnT-VB /db_xref="GeneID:146664" /db_xref="HGNC:24140" CDS 692..2920 /dnas_title="mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase, isozyme B" /gene="MGAT5B" /gene_synonym=GnT-IX /gene_synonym=GnT-VB /codon_start=1 /product="mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase, isozyme B" /protein_id="AAI56824.1" /db_xref="GI:162319294" /db_xref="GeneID:146664" /db_xref="HGNC:24140" /translation="MHSFVKHLCSRYVVERQGTMALPALLTRLLPLRRLFVLGIGFFT LCFLMTSLGGQFSARRLGDSPFTIRTEVMGGPESRGVLRKMSDLLELMVKRMDALARL ENSSELHRAGGDLHFPADRMPPGAGLMERIQAIAQNVSDIAVKVDQILRHSLLLHSKV SEGRRDQCEAPSDPKFPDCSGKVEWMRARWTSDPCYAFFGVDGTECSFLIYLSEVEWF CPPLPWRNQTAAQRAPKPLPKVQAVFRSNLSHLLDLMGSGKESLIFMKKRTKRLTAQW ALAAQRLAQKLGATQRDQKQILVHIGFLTEESGDVFSPRVLKGGPLGEMVQWADILTA LYVLGHGLRVTVSLKELQSNLGVPPGRGSCPLTMPLPFDLIYTDYHGLQQMKRHMGLS FKKYRCRIRVIDTFGTEPAYNHEEYATLHGYRTNWGYWNLNPKQFMTMFPHTPDNSFM GFVSEELNETEKRLIKGGKASNMAVVYGKEASIWKGKEKFLGILNKYMEIHGTVYYES QRPPEVPAFVKNHGLLPQPEFQQLLRKAKLFIGFGFPYEGPAPLEAIANGCIFLQSRF SPPHSSLNHEFFRGKPTSREVFSQHPYAENFIGKPHVWTVDYNNSEEFEAAIKAIMRT QVDPYLPYEYTCEGMLERIHAYIQHQDFCRAPDPALPEAHAPQSPFVLAPNATHLEWA RNTSLAPGAWPPAHALRAWLAVPGRACTDTCLDHGLICEPSFFPFLNSQDAFLKLQVP CDSTESEMNHLYPAFAQPGQECYLQKEPLLFSCAGSNTKYRRLCPCRDFRKGQVALCQ GCL" misc_feature 692..2920 /note="MGAT5B coding region" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg ccggcgcctg ggggactcgc cattcaccat 721 ccgcacagaa gtgatggggg gccccgagtc ccgcggcgtc ctgcgcaaga tgagcgacct 781 gctggagctg atggtgaagc gcatggacgc actggccagg ctggagaaca gcagtgagct 841 gcaccgggcc ggcggcgacc tgcactttcc cgcagacagg atgccccctg gggccggcct 901 catggagcgg atccaggcta ttgcccagaa cgtctccgac atcgctgtga aggtggacca 961 gatcctgcgc cacagtctgc tcctgcacag caaggtgtca gaaggccggc gggaccagtg 1021 tgaggcaccc agtgacccca agttccctga ctgctcaggg aaggtggagt ggatgcgtgc 1081 ccgctggacc tctgacccct gctacgcctt ctttggggtg gacggcaccg agtgctcctt 1141 cctcatctac ctcagtgagg tcgagtggtt ctgccccccg ctgccctgga ggaaccagac 1201 ggctgcccag agggcaccca agcccctccc caaagtccag gcagttttcc gaagcaacct 1261 gtcccacctt ctggacctga tgggcagcgg gaaggagtcc ctgatcttca tgaagaagcg 1321 gaccaagagg ctcacagccc agtgggcgct ggctgcccag cgcctggcac agaagctggg 1381 ggccacccag agggaccaga agcagatcct ggtccacatc ggcttcctga cggaggagtc 1441 cggggacgtg ttcagccctc gggtcctgaa gggcgggccc ctaggggaga tggtgcagtg 1501 ggcggacatt ctgactgcac tctatgtcct gggccatggc ctgcgggtca cagtctccct 1561 gaaggagctg cagagtaact taggggtacc gccaggccgg ggaagctgcc cgctcaccat 1621 gcccctgccc ttcgacctca tctacaccga ctaccacggc ctgcagcaga tgaagcggca 1681 catgggactc tccttcaaga agtaccggtg ccgaatcagg gtcatcgaca ccttcgggac 1741 ggaacctgcg tacaaccacg aggagtacgc cacgctgcac ggctaccgga ccaactgggg 1801 ctactggaac ctcaacccca agcagttcat gaccatgttt cctcataccc ccgacaactc 1861 cttcatgggc ttcgtgtccg aggagctcaa cgagacggag aagcggctca tcaaaggcgg 1921 caaggccagc aacatggccg tggtgtacgg caaggaggcg agcatctgga aggggaagga 1981 gaagttcctg ggcatcctga acaaatacat ggagatccat ggcaccgtgt actacgagag 2041 ccagcggccc cccgaggtgc cagcctttgt gaagaaccac ggcctcttac cgcagcctga 2101 gtttcagcag ctgctgcgca aggccaaact cttcatcggg tttggcttcc cctacgaggg 2161 ccccgccccc ctggaggcca tcgccaatgg ttgcatcttc ctgcagtccc gcttcagccc 2221 gccccacagc tccctcaacc acgagttctt ccgaggcaag cccacctcca gagaggtgtt 2281 ctcccagcat ccctacgcgg agaacttcat cggcaagccc cacgtgtgga cagtcgacta 2341 caacaactca gaggagtttg aagcagccat caaggccatt atgagaactc aggtagaccc 2401 ctacctaccc tacgagtaca cctgcgaggg gatgctggag cggatccacg cctacatcca 2461 gcaccaggac ttctgcagag ctccagaccc tgccctacca gaggcccacg ccccgcagag 2521 cccctttgtc ctggccccca atgccaccca cctcgagtgg gctcggaaca ccagcttggc 2581 tcctggggcc tggccccccg cgcacgccct gcgggcctgg ctggccgtgc ctgggagggc 2641 ctgcaccgac acctgcctgg accacgggct aatctgtgag ccctccttct tccccttcct 2701 gaacagccag gacgccttcc tcaagctgca ggtgccctgt gacagcaccg agtcggagat 2761 gaaccacctg tacccggcgt tcgcccagcc tggccaggag tgctacctgc agaaggagcc 2821 tctgctcttc agctgcgccg gctccaacac caagtaccgc cggctctgcc cctgccgcga 2881 cttccgcaag ggccaggtgg ccttgtgcca gggctgtctg TAGGACCCAG CTTTCTTGTA 2941 CAAAGTTGGC ATTATAAGAA AGCATTGCTT ATCAATTTGT TGCAACGAAC AGGTCACTAT 3001 CAGTCAAAAT AAAATCATTA TTTGCCATCC AGCTGATATC CCCTATAGTG AGTCGTATTA 3061 CATGGTCATA GCTGTTTCCT GGCAGCTCTG GCCCGTGTCT CAAAATCTCT GATGTTACAT 3121 TGCACAAGAT AAAATAATAT CATCATGAAC AATAAAACTG TCTGCTTACA TAAACAGTAA 3181 TACAAGGGGT GTTATGAGCC ATATTCAACG GGAAACGTCG AGGCCGCGAT TAAATTCCAA 3241 CATGGATGCT GATTTATATG GGTATAAATG GGCTCGCGAT AATGTCGGGC AATCAGGTGC 3301 GACAATCTAT CGCTTGTATG GGAAGCCCGA TGCGCCAGAG TTGTTTCTGA AACATGGCAA 3361 AGGTAGCGTT GCCAATGATG TTACAGATGA GATGGTCAGA CTAAACTGGC TGACGGAATT 3421 TATGCCTCTT CCGACCATCA AGCATTTTAT CCGTACTCCT GATGATGCAT GGTTACTCAC 3481 CACTGCGATC CCCGGAAAAA CAGCATTCCA GGTATTAGAA GAATATCCTG ATTCAGGTGA 3541 AAATATTGTT GATGCGCTGG CAGTGTTCCT GCGCCGGTTG CATTCGATTC CTGTTTGTAA 3601 TTGTCCTTTT AACAGCGATC GCGTATTTCG TCTCGCTCAG GCGCAATCAC GAATGAATAA 3661 CGGTTTGGTT GATGCGAGTG ATTTTGATGA CGAGCGTAAT GGCTGGCCTG TTGAACAAGT 3721 CTGGAAAGAA ATGCATAAAC TTTTGCCATT CTCACCGGAT TCAGTCGTCA CTCATGGTGA 3781 TTTCTCACTT GATAACCTTA TTTTTGACGA GGGGAAATTA ATAGGTTGTA TTGATGTTGG 3841 ACGAGTCGGA ATCGCAGACC GATACCAGGA TCTTGCCATC CTATGGAACT GCCTCGGTGA 3901 GTTTTCTCCT TCATTACAGA AACGGCTTTT TCAAAAATAT GGTATTGATA ATCCTGATAT 3961 GAATAAATTG CAGTTTCATT TGATGCTCGA TGAGTTTTTC TAATCAGAAT TGGTTAATTG 4021 GTTGTAACAC TGGCAGAGCA TTACGCTGAC TTGACGGGAC GGCGCAAGCT CATGACCAAA 4081 ATCCCTTAAC GTGAGTTACG CGTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA 4141 AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC 4201 ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT 4261 AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC CGTAGTTAGG 4321 CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC 4381 AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT 4441 ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA 4501 GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT 4561 TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG 4621 CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA 4681 CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA 4741 CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT //