STEP 1: Searching for a gene name based on enzyme specificity:
If you are searching for an enzyme with a known specificity but do not know the gene symbol there are two good ways to find the candidate gene: the HUGO Gene Nomenclature Committee "GeneNames" website and/or UniProt. To search the HGNC website navigate to http://www.genenames.org/ and enter the specificity of the enzyme. The example below used the query "sialyltransferase". After entering "sialyltransferase ito the search box click the magnifying glass icon.
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STEP 2: Browse search results for desired enzyme specificity:
The search results will return a list of matches to the query with enzyme names and "Approved" gene symbols. Note the respective symbols for searching the the expression repository site.


STEP 3: Alternatively search the UniProt site:
To search the UniProt website navigate to http://www.uniprot.org/ and enter the specificity of the enzyme. The example below used the query "sialyltransferase". After entering "sialyltransferase" and "homo sapiens" into the search box click the search button.

STEP 4: Browse search results for desired enzyme specificity:
The search results will return a list of matches to the query with enzyme names and Gene names (gene symbols). Note the respective symbols for searching the the Expression Repository site.


STEP 5: Once you have the Gene Symbol, find the respective GeneRecord in the Expression Repository:
Using the Gene Symbol navigate to the Alphabetical index of the Expression Repository and find the link to the respective gene.

STEP 6: Clicking on a gene symbol (gene name) will show the corresponding gene record. Each gene record contains annotation information for the given gene as well as links for the respective databases (upper panel). The table in the lower panel provides design information for all constructs including sequence data (in GenBank format) and clone IDs for the respective pDONR constructs, mammalian and bacterial expression vectors in DNASU, and baculovirus expression vectors, available from the Jarvis lab.

STEP 7: To acquire the corresponding plasmid clone, copy the clone ID and click the link at the column header that says "DNASU Clone ID". This will take you to plasmid search page for DNASU.

PLEASE NOTE: All baculovirus stocks are obtained directly from the Jarvis lab not from DNASU.

STEP 8: Once you are at the DNASU search page paste the Clone ID into the search page and click search:

STEP 9: The construct plasmid record will be shown with additional links to annotation information. Once you confirm that the construct encodes your gene of interest you can click the "Add To Cart" button and complete the purchase of the clone.