LOCUS       pET16-DEST              7428 bp    DNA     circular     10-JUN-2011
FEATURES             Location/Qualifiers
     misc_feature    5323..5352
                     /note="His10-tag"
     misc_feature    5365..5376
                     /note="X-factor"
     misc_feature    5230..5245
                     /note="T7 Promoter"
     misc_feature    5249..5273
                     /note="Lac O"
     misc_feature    7155..7216
                     /note="T7 Terminator"
     gene            5625..6284
                     /note="CM"
     misc_recomb     complement(5392..5516)
                     /label=attR1
                     /note="attR1 site"
     gene            6626..6931
                     /note="ccdB"
     misc_recomb     6972..7096
                     /label=attR2
                     /note="attR2 site"
     misc_feature    complement(3761..4720)
                     /note="Lac I"
     misc_feature    209..1069
                     /note="Ap"
     misc_feature    1224..1843
                     /note="Ori"
ORIGIN      
          1 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
         61 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
        121 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
        181 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
        241 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
        301 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
        361 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
        421 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg
        481 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
        541 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
        601 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
        661 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
        721 accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact
        781 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
        841 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
        901 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
        961 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
       1021 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
       1081 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
       1141 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
       1201 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
       1261 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
       1321 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
       1381 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
       1441 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
       1501 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
       1561 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
       1621 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
       1681 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
       1741 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
       1801 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
       1861 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
       1921 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
       1981 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
       2041 tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc
       2101 gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc
       2161 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
       2221 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
       2281 cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga
       2341 tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc
       2401 ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg
       2461 tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca
       2521 cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac
       2581 tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg
       2641 ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga
       2701 acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga
       2761 agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc
       2821 gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg
       2881 tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga
       2941 tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg
       3001 tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc
       3061 cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg
       3121 acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt
       3181 ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt
       3241 taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg
       3301 cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat
       3361 aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc
       3421 ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt
       3481 gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat
       3541 cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg
       3601 tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg
       3661 cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gagatcccgg
       3721 tgcctaatga gtgagctaac ttacattaat tgcgttgcgc tcactgcccg ctttccagtc
       3781 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
       3841 gcgtattggg cgccagggtg gtttttcttt tcaccagtga gacgggcaac agctgattgc
       3901 ccttcaccgc ctggccctga gagagttgca gcaagcggtc cacgctggtt tgccccagca
       3961 ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata acatgagctg tcttcggtat
       4021 cgtcgtatcc cactaccgag atatccgcac caacgcgcag cccggactcg gtaatggcgc
       4081 gcattgcgcc cagcgccatc tgatcgttgg caaccagcat cgcagtggga acgatgccct
       4141 cattcagcat ttgcatggtt tgttgaaaac cggacatggc actccagtcg ccttcccgtt
       4201 ccgctatcgg ctgaatttga ttgcgagtga gatatttatg ccagccagcc agacgcagac
       4261 gcgccgagac agaacttaat gggcccgcta acagcgcgat ttgctggtga cccaatgcga
       4321 ccagatgctc cacgcccagt cgcgtaccgt cttcatggga gaaaataata ctgttgatgg
       4381 gtgtctggtc agagacatca agaaataacg ccggaacatt agtgcaggca gcttccacag
       4441 caatggcatc ctggtcatcc agcggatagt taatgatcag cccactgacg cgttgcgcga
       4501 gaagattgtg caccgccgct ttacaggctt cgacgccgct tcgttctacc atcgacacca
       4561 ccacgctggc acccagttga tcggcgcgag atttaatcgc cgcgacaatt tgcgacggcg
       4621 cgtgcagggc cagactggag gtggcaacgc caatcagcaa cgactgtttg cccgccagtt
       4681 gttgtgccac gcggttggga atgtaattca gctccgccat cgccgcttcc actttttccc
       4741 gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc tgataagaga
       4801 caccggcata ctctgcgaca tcgtataacg ttactggttt cacattcacc accctgaatt
       4861 gactctcttc cgggcgctat catgccatac cgcgaaaggt tttgcgccat tcgatggtgt
       4921 ccgggatctc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg
       4981 ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac
       5041 agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg
       5101 aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca
       5161 cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc
       5221 ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag
       5281 aaataatttt gtttaacttt aagaaggaga tataccatgg gccatcatca tcatcatcat
       5341 catcatcatc acagcagcgg ccatatcgaa ggtcgtcata tgctcgaATC AACAAGTTTG
       5401 TACAAAAAAG CTGAACGAGA AACGTAAAAT GATATAAATA TCAATATATT AAATTAGATT
       5461 TTGCATAAAA AACAGACTAC ATAATACTGT AAAACACAAC ATATCCAGTC ACTATGGCGG
       5521 CCGCATTAGG CACCCCAGGC TTTACACTTT ATGCTTCCGG CTCGTATAAT GTGTGGATTT
       5581 TGAGTTAGGA TCCGTCGAGA TTTTCAGGAG CTAAGGAAGC TAAAATGGAG AAAAAAATCA
       5641 CTGGATATAC CACCGTTGAT ATATCCCAAT GGCATCGTAA AGAACATTTT GAGGCATTTC
       5701 AGTCAGTTGC TCAATGTACC TATAACCAGA CCGTTCAGCT GGATATTACG GCCTTTTTAA
       5761 AGACCGTAAA GAAAAATAAG CACAAGTTTT ATCCGGCCTT TATTCACATT CTTGCCCGCC
       5821 TGATGAATGC TCATCCGGAA TTCCGTATGG CAATGAAAGA CGGTGAGCTG GTGATATGGG
       5881 ATAGTGTTCA CCCTTGTTAC ACCGTTTTCC ATGAGCAAAC TGAAACGTTT TCATCGCTCT
       5941 GGAGTGAATA CCACGACGAT TTCCGGCAGT TTCTACACAT ATATTCGCAA GATGTGGCGT
       6001 GTTACGGTGA AAACCTGGCC TATTTCCCTA AAGGGTTTAT TGAGAATATG TTTTTCGTCT
       6061 CAGCCAATCC CTGGGTGAGT TTCACCAGTT TTGATTTAAA CGTGGCCAAT ATGGACAACT
       6121 TCTTCGCCCC CGTTTTCACC ATGGGCAAAT ATTATACGCA AGGCGACAAG GTGCTGATGC
       6181 CGCTGGCGAT TCAGGTTCAT CATGCCGTTT GTGATGGCTT CCATGTCGGC AGAATGCTTA
       6241 ATGAATTACA ACAGTACTGC GATGAGTGGC AGGGCGGGGC GTAAAGATCT GGATCCGGCT
       6301 TACTAAAAGC CAGATAACAG TATGCGTATT TGCGCGCTGA TTTTTGCGGT ATAAGAATAT
       6361 ATACTGATAT GTATACCCGA AGTATGTCAA AAAGAGGTAT GCTATGAAGC AGCGTATTAC
       6421 AGTGACAGTT GACAGCGACA GCTATCAGTT GCTCAAGGCA TATATGATGT CAATATCTCC
       6481 GGTCTGGTAA GCACAACCAT GCAGAATGAA GCCCGTCGTC TGCGTGCCGA ACGCTGGAAA
       6541 GCGGAAAATC AGGAAGGGAT GGCTGAGGTC GCCCGGTTTA TTGAAATGAA CGGCTCTTTT
       6601 GCTGACGAGA ACAGGGGCTG GTGAAATGCA GTTTAAGGTT TACACCTATA AAAGAGAGAG
       6661 CCGTTATCGT CTGTTTGTGG ATGTACAGAG TGATATTATT GACACGCCCG GGCGACGGAT
       6721 GGTGATCCCC CTGGCCAGTG CACGTCTGCT GTCAGATAAA GTCTCCCGTG AACTTTACCC
       6781 GGTGGTGCAT ATCGGGGATG AAAGCTGGCG CATGATGACC ACCGATATGG CCAGTGTGCC
       6841 GGTCTCCGTT ATCGGGGAAG AAGTGGCTGA TCTCAGCCAC CGCGAAAATG ACATCAAAAA
       6901 CGCCATTAAC CTGATGTTCT GGGGAATATA AATGTCAGGC TCCCTTATAC ACAGCCAGTC
       6961 TGCAGGTCGA CCATAGTGAC TGGATATGTT GTGTTTTACA GTATTATGTA GTCTGTTTTT
       7021 TATGCAAAAT CTAATTTAAT ATATTGATAT TTATATCATT TTACGTTTCT CGTTCAGCTT
       7081 TCTTGTACAA AGTGGTTGAT tcgaggatcc ggctgctaac aaagcccgaa aggaagctga
       7141 gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt
       7201 cttgaggggt tttttgctga aaggaggaac tatatccgga tatcccgcaa gaggcccggc
       7261 agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg
       7321 atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat
       7381 aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
//
