LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(1559..1655) /label=attL2 /note="attL2 (c)" misc_signal complement(1670..1689) /note="T7 Promoter/priming site (c)" primer_bind 1697..1713 /note="M13 Reverse priming site" gene 1826..2635 /note="Kanamycin resistance gene" rep_origin 2756..3429 /note="pUC origin" vector join(1567..3432,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 671..691 /note="TEV site" misc_feature 692..1552 /note="BGT66_T01a" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg cTATAAAGCA CCAACAGTTT TTTCTCAAAA 721 TGAAGCATCA TTATTAAATC AATTAAAAAA TATAGCCAAT AGAGAAGATT ATGTGGTAAC 781 TTGGTGGGAT TATGGTTATC CTGTGCGTTA TTATAGTGAT GTGAAAACTT TAGTAGATGG 841 TGGAAAGCAT TTAGGTAAGG ATAATTTTTT CCCTTCTTTT GCTTTAAGCA AAGATGAACA 901 AGCTGCAGCT AATATGGCAA GACTTAGTGT AGAATATACA GAAAAAAGCT TTTATGCTCC 961 GCAAAATGAT ATTTTAAAAA CAGACATTTT ACAAGCCATG ATGAAAGATT ATAATCAAAG 1021 CAATGTGGAT TTGTTTCTAG CTTCATTATC AAAACCTGAT TTTAAAATCG ATACACCAAA 1081 AACTCGTGAT ATTTATCTTT ATATGCCCGC TAGAATGTCT TTGATTTTTT CTACGGTGGC 1141 TAGTTTTTCT TTTATTAATT TAGATACAGG AGTTTTGGAT AAACCTTTTA CCTTTAGCAC 1201 AGCTTATCCA CTTGATGTTA AAAATGGAGA AATTTATCTT AGCAACGGAG TGGTTTTAAG 1261 CGATGATTTT AGAAGTTTTA AAATAGGTGA TAATGTGGTT TCTGTAAATA GTATCGTAGA 1321 GATTAATTCT ATTAAACAAG GTGAATACAA AATCACTCCA ATTGATGATA AGGCTCAGTT 1381 TTATATTTTT TATTTAAAGG ATAGTGCTAT TCCTTACGCA CAATTTATTT TAATGGATAA 1441 AACCATGTTT AATAGTGCTT ATGTGCAAAT GTTTTTTTTA GGAAATTATG ATAAGAATTT 1501 ATTTGACTTG GTGATTAATT CTAGAGATGC TAAGGTTTTT AAACTTAAAA TTTAGGACCC 1561 AGCTTTCTTG TACAAAGTTG GCATTATAAG AAAGCATTGC TTATCAATTT GTTGCAACGA 1621 ACAGGTCACT ATCAGTCAAA ATAAAATCAT TATTTGCCAT CCAGCTGATA TCCCCTATAG 1681 TGAGTCGTAT TACATGGTCA TAGCTGTTTC CTGGCAGCTC TGGCCCGTGT CTCAAAATCT 1741 CTGATGTTAC ATTGCACAAG ATAAAATAAT ATCATCATGA ACAATAAAAC TGTCTGCTTA 1801 CATAAACAGT AATACAAGGG GTGTTATGAG CCATATTCAA CGGGAAACGT CGAGGCCGCG 1861 ATTAAATTCC AACATGGATG CTGATTTATA TGGGTATAAA TGGGCTCGCG ATAATGTCGG 1921 GCAATCAGGT GCGACAATCT ATCGCTTGTA TGGGAAGCCC GATGCGCCAG AGTTGTTTCT 1981 GAAACATGGC AAAGGTAGCG TTGCCAATGA TGTTACAGAT GAGATGGTCA GACTAAACTG 2041 GCTGACGGAA TTTATGCCTC TTCCGACCAT CAAGCATTTT ATCCGTACTC CTGATGATGC 2101 ATGGTTACTC ACCACTGCGA TCCCCGGAAA AACAGCATTC CAGGTATTAG AAGAATATCC 2161 TGATTCAGGT GAAAATATTG TTGATGCGCT GGCAGTGTTC CTGCGCCGGT TGCATTCGAT 2221 TCCTGTTTGT AATTGTCCTT TTAACAGCGA TCGCGTATTT CGTCTCGCTC AGGCGCAATC 2281 ACGAATGAAT AACGGTTTGG TTGATGCGAG TGATTTTGAT GACGAGCGTA ATGGCTGGCC 2341 TGTTGAACAA GTCTGGAAAG AAATGCATAA ACTTTTGCCA TTCTCACCGG ATTCAGTCGT 2401 CACTCATGGT GATTTCTCAC TTGATAACCT TATTTTTGAC GAGGGGAAAT TAATAGGTTG 2461 TATTGATGTT GGACGAGTCG GAATCGCAGA CCGATACCAG GATCTTGCCA TCCTATGGAA 2521 CTGCCTCGGT GAGTTTTCTC CTTCATTACA GAAACGGCTT TTTCAAAAAT ATGGTATTGA 2581 TAATCCTGAT ATGAATAAAT TGCAGTTTCA TTTGATGCTC GATGAGTTTT TCTAATCAGA 2641 ATTGGTTAAT TGGTTGTAAC ACTGGCAGAG CATTACGCTG ACTTGACGGG ACGGCGCAAG 2701 CTCATGACCA AAATCCCTTA ACGTGAGTTA CGCGTCGTTC CACTGAGCGT CAGACCCCGT 2761 AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA 2821 AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT 2881 TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTTC TTCTAGTGTA 2941 GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT 3001 AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC 3061 AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA 3121 GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA 3181 AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG 3241 AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT 3301 CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG 3361 CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT 3421 TGCTCACATG TT //