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FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(1949..2045) /label=attL2 /note="attL2 (c)" misc_signal complement(2060..2079) /note="T7 Promoter/priming site (c)" primer_bind 2087..2103 /note="M13 Reverse priming site" gene 2216..3025 /note="Kanamycin resistance gene" rep_origin 3146..3819 /note="pUC origin" vector join(1957..3822,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 671..691 /note="TEV site" misc_feature 692..1942 /note="BGT04_T02a" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg cCGCGTAGCT ATGATTTCCA TGCACACCTC 721 TCCATTGCAG CAGCCCGGAA CTGGTGATTC AGGCGGCATG AACGTCTACA TTCTTTCGAC 781 CGCGACTGAG CTAGCGAAAC AGGGTATCGA GGTCGATATT TACACTCGTG CCACGAGGCC 841 TTCTCAGGGT GAGATCGTGA GAGTAGCTGA GAATTTGCGG GTCATTAATA TCGCTGCGGG 901 GCCGTATGAG GGGCTTTCCA AAGAGGAGCT TCCTACTCAG TTGGCGGCGT TTACCGGCGG 961 AATGTTGTCG TTTACGCGCC GGGAGAAGGT TACTTATGAT CTGATCCATT CTCACTATTG 1021 GCTGTCTGGT CAGGTGGGGT GGTTGCTGCG CGATTTGTGG CGGATTCCCC TTATTCATAC 1081 GGCACACACT TTGGCGGCGG TGAAGAATTC TTATCGGGAT GATTCGGACA CTCCGGAGTC 1141 GGAGGCGCGT CGCATTTGTG AGCAGCAGCT GGTGGATAAC GCTGACGTGT TGGCGGTGAA 1201 CACTCAGGAG GAGATGCAGG ATTTGATGCA TCACTACGAT GCGGATCCGG ATCGGATTTC 1261 TGTGGTGTCA CCGGGTGCGG ACGTGGAACT TTATAGCCCT GGAAATGATC GCGCGACGGA 1321 ACGTTCCCGT CGTGAGCTGG GCATTCCGCT GCACACAAAG GTAGTGGCTT TTGTGGGTCG 1381 GTTGCAGCCG TTTAAGGGCC CGCAGGTGCT GATCAAGGCG GTTGCGGCGT TGTTTGATCG 1441 CGATCCGGAC CGAAATCTGC GCGTCATTAT TTGTGGCGGC CCTTCTGGTC CGAATGCGAC 1501 ACCGGATACC TATAGGCATA TGGCAGAGGA ACTGGGCGTC GAAAAGCGAA TTCGCTTTTT 1561 GGACCCGCGC CCGCCGAGCG AGCTAGTGGC CGTGTATCGG GCGGCGGACA TCGTGGCCGT 1621 GCCAAGTTTT AATGAGTCCT TCGGACTCGT CGCCATGGAG GCGCAAGCCA GCGGCACACC 1681 GGTCATTGCG GCCCGGGTTG GCGGCCTGCC CATCGCAGTC GCGGAAGGGG AGACGGGATT 1741 GCTTGTCGAC GGCCACTCCC CGCATGCCTG GGCCGACGCC TTAGCCACAC TCTTGGACGA 1801 TGACGAAACG CGCATCAGAA TGGGTGAAGA CGCCGTCGAA CACGCCAGAA CATTCTCCTG 1861 GGCGGCCACC GCCGCACAGC TATCGTCGCT GTACAACGAC GCTATTGCCA ACGAAAATGT 1921 CGACGGTGAA ACGCATCACG GCTAGGACCC AGCTTTCTTG TACAAAGTTG GCATTATAAG 1981 AAAGCATTGC TTATCAATTT GTTGCAACGA ACAGGTCACT ATCAGTCAAA ATAAAATCAT 2041 TATTTGCCAT CCAGCTGATA TCCCCTATAG TGAGTCGTAT TACATGGTCA TAGCTGTTTC 2101 CTGGCAGCTC TGGCCCGTGT CTCAAAATCT CTGATGTTAC ATTGCACAAG ATAAAATAAT 2161 ATCATCATGA ACAATAAAAC TGTCTGCTTA CATAAACAGT AATACAAGGG GTGTTATGAG 2221 CCATATTCAA CGGGAAACGT CGAGGCCGCG ATTAAATTCC AACATGGATG CTGATTTATA 2281 TGGGTATAAA TGGGCTCGCG ATAATGTCGG GCAATCAGGT GCGACAATCT ATCGCTTGTA 2341 TGGGAAGCCC GATGCGCCAG AGTTGTTTCT GAAACATGGC AAAGGTAGCG TTGCCAATGA 2401 TGTTACAGAT GAGATGGTCA GACTAAACTG GCTGACGGAA TTTATGCCTC TTCCGACCAT 2461 CAAGCATTTT ATCCGTACTC CTGATGATGC ATGGTTACTC ACCACTGCGA TCCCCGGAAA 2521 AACAGCATTC CAGGTATTAG AAGAATATCC TGATTCAGGT GAAAATATTG TTGATGCGCT 2581 GGCAGTGTTC CTGCGCCGGT TGCATTCGAT TCCTGTTTGT AATTGTCCTT TTAACAGCGA 2641 TCGCGTATTT CGTCTCGCTC AGGCGCAATC ACGAATGAAT AACGGTTTGG TTGATGCGAG 2701 TGATTTTGAT GACGAGCGTA ATGGCTGGCC TGTTGAACAA GTCTGGAAAG AAATGCATAA 2761 ACTTTTGCCA TTCTCACCGG ATTCAGTCGT CACTCATGGT GATTTCTCAC TTGATAACCT 2821 TATTTTTGAC GAGGGGAAAT TAATAGGTTG TATTGATGTT GGACGAGTCG GAATCGCAGA 2881 CCGATACCAG GATCTTGCCA TCCTATGGAA CTGCCTCGGT GAGTTTTCTC CTTCATTACA 2941 GAAACGGCTT TTTCAAAAAT ATGGTATTGA TAATCCTGAT ATGAATAAAT TGCAGTTTCA 3001 TTTGATGCTC GATGAGTTTT TCTAATCAGA ATTGGTTAAT TGGTTGTAAC ACTGGCAGAG 3061 CATTACGCTG ACTTGACGGG ACGGCGCAAG CTCATGACCA AAATCCCTTA ACGTGAGTTA 3121 CGCGTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC 3181 TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT 3241 TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC 3301 GCAGATACCA AATACTGTTC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC 3361 TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG 3421 CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG 3481 GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA 3541 ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC 3601 GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG 3661 GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG 3721 ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT 3781 TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TT //