LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2255..2351) /label=attL2 /note="attL2 (c)" misc_signal complement(2366..2385) /note="T7 Promoter/priming site (c)" primer_bind 2393..2409 /note="M13 Reverse priming site" gene 2522..3331 /note="Kanamycin resistance gene" rep_origin 3452..4125 /note="pUC origin" vector join(2263..4128,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" misc_feature 692..2248 /note="BGT39_T02a" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg cAGCCAAGCC CTACCTGTTC GTGACCAGGG 721 GCGGGACCAG GGGATTTTCG CTGGTACTCT CCCGCCCGCA CCGCCAAAGT TCAAGTGGAC 781 CCGCCTGGAT ACCTATACCT GGGCGATCAT CGCGGTGTTT GCTTTAGTCA CGCGTTTTAC 841 AGGTTTGAGC AGTGCAACCG CCTCAGGCAC CCCAGTTTTT GATGAAAAAC ACTACGTCCC 901 ACAAGCCTGG GACATGGTCC GCTCCTGGAT CAACCCCATC ACCGGCGGCA TTGAATCCAA 961 CCCCGGCTAC GGACTCGTCG TTCACCCACC GTTGGCCAAA CAACTTGAAG CACTCGGTGA 1021 ATGGGTCTTC GGATACACCC CCCTCGGCTG GCGCATCATG GTCGCCATTT TCGGAACACT 1081 CACCATTTTC GCCATCATGG CAATCGCGCG GCGCCTCAGC GGATCCACCA TGGTGACTTT 1141 CATCGCCGGA ATTCTCGCGC TTGCCGACGG CGTCCTCCTC GTCTCATCAC GCTTCGGCAT 1201 GCTGGACATC TTCCTTGTTT TCTTCATCAC CGCAGCAGCA TGGGCATTGA TCCGAGACCA 1261 CCAACAAATG CACCAACGCC TCAACGATTT ACTCCTCACC AACGGCCAAA TCACCAAAGA 1321 CTTTGGCCCC CGATTCGGCT TCCGATGGTG GCGTTTCACC ACAGGTGTAT TCCTTGGTCT 1381 CGCGCTCTCC GTCAAATGGT CCGGACTGTA CTACATCGCA TTCTTCGGCC TGACCAGCGT 1441 GTTCCTAGAC TTGTGGCTGC GCAAACGCTA CGGCGTCCGA CGCTACGTCA CCGGCACCCT 1501 AAAAAATGAC GTCATCCCAG CGCTCGGCTC CTTGGTGATC ATCCCTGCCC TGCTTTATAT 1561 CTGGAGCTGG CGAGCCTGGT TCGCCTCCGA AACCTCCGTC TACCGCCACG CCAAAACAGA 1621 CGGCACCATC ACAGAAGACT CCATCCTGCA GCTCTTCCCT GAGTCAATTG CGGGCTGGAT 1681 CCACTATCAC ATCAGCGTCC TGGAATTCCA CGGCTCACTG ACCACATCCT CAGGTCACAG 1741 CCACCCCTGG GATTCCAAGC CGTGGGCCTG GCTTGTCTCC GGTCGACCCA TCCTCTACTT 1801 CTCCTCCACC GACATCTCCT GCGACGTTGG CGGCACCTGC CGACGCATGA TCTACCTCTT 1861 CGGCACGCCC GCCATTTGGT GGCTGACAGT CCCAGTCATT CTGTGGGCGC TGTGGTCTTT 1921 CTTCGCACGT CGGAGTCGTG GCTATGTTGT CCCGCTGGTT GCTTTCGCCG CAGGATTTTT 1981 ACCGTGGCTT GCAGCATACG ACCGCCAAAT GTACTTCTTC TACGCCACCG CCCTAGTTCC 2041 ATTCACCATC ATCATGTTGG CACTCGCCTG CGGTGAACTG TGGGGCCGTG GAAAAATGAC 2101 CCCCACTGGC TTAACCCGCG GATCAATGGC AGTGGTCACC TACATTTCCC TCGTGGTGAT 2161 GATGTTTCTC GCGTTTTCGC CACTGTTTTA CGGATTTGTC ATCCCAGATT ATGTCTACGA 2221 ATCCTTGATG TGGTTCCCAA GCTGGCGCTA GGACCCAGCT TTCTTGTACA AAGTTGGCAT 2281 TATAAGAAAG CATTGCTTAT CAATTTGTTG CAACGAACAG GTCACTATCA GTCAAAATAA 2341 AATCATTATT TGCCATCCAG CTGATATCCC CTATAGTGAG TCGTATTACA TGGTCATAGC 2401 TGTTTCCTGG CAGCTCTGGC CCGTGTCTCA AAATCTCTGA TGTTACATTG CACAAGATAA 2461 AATAATATCA TCATGAACAA TAAAACTGTC TGCTTACATA AACAGTAATA CAAGGGGTGT 2521 TATGAGCCAT ATTCAACGGG AAACGTCGAG GCCGCGATTA AATTCCAACA TGGATGCTGA 2581 TTTATATGGG TATAAATGGG CTCGCGATAA TGTCGGGCAA TCAGGTGCGA CAATCTATCG 2641 CTTGTATGGG AAGCCCGATG CGCCAGAGTT GTTTCTGAAA CATGGCAAAG GTAGCGTTGC 2701 CAATGATGTT ACAGATGAGA TGGTCAGACT AAACTGGCTG ACGGAATTTA TGCCTCTTCC 2761 GACCATCAAG CATTTTATCC GTACTCCTGA TGATGCATGG TTACTCACCA CTGCGATCCC 2821 CGGAAAAACA GCATTCCAGG TATTAGAAGA ATATCCTGAT TCAGGTGAAA ATATTGTTGA 2881 TGCGCTGGCA GTGTTCCTGC GCCGGTTGCA TTCGATTCCT GTTTGTAATT GTCCTTTTAA 2941 CAGCGATCGC GTATTTCGTC TCGCTCAGGC GCAATCACGA ATGAATAACG GTTTGGTTGA 3001 TGCGAGTGAT TTTGATGACG AGCGTAATGG CTGGCCTGTT GAACAAGTCT GGAAAGAAAT 3061 GCATAAACTT TTGCCATTCT CACCGGATTC AGTCGTCACT CATGGTGATT TCTCACTTGA 3121 TAACCTTATT TTTGACGAGG GGAAATTAAT AGGTTGTATT GATGTTGGAC GAGTCGGAAT 3181 CGCAGACCGA TACCAGGATC TTGCCATCCT ATGGAACTGC CTCGGTGAGT TTTCTCCTTC 3241 ATTACAGAAA CGGCTTTTTC AAAAATATGG TATTGATAAT CCTGATATGA ATAAATTGCA 3301 GTTTCATTTG ATGCTCGATG AGTTTTTCTA ATCAGAATTG GTTAATTGGT TGTAACACTG 3361 GCAGAGCATT ACGCTGACTT GACGGGACGG CGCAAGCTCA TGACCAAAAT CCCTTAACGT 3421 GAGTTACGCG TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG 3481 AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC 3541 GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG 3601 CAGAGCGCAG ATACCAAATA CTGTTCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA 3661 GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC 3721 CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC 3781 GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA 3841 CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG 3901 AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT 3961 TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA 4021 GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC 4081 GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTT //