LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(3086..3182) /label=attL2 /note="attL2 (c)" misc_signal complement(3197..3216) /note="T7 Promoter/priming site (c)" primer_bind 3224..3240 /note="M13 Reverse priming site" gene 3353..4162 /note="Kanamycin resistance gene" rep_origin 4283..4956 /note="pUC origin" vector join(3094..4959,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" misc_feature 692..3079 /note="BGT35_T01b" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg cTCACAACCT ATTTTTAACG ATAAGCAATT 721 TCAGGAAGCG CTTTCACGTC AGTGGCAGCG TTATGGCTTA AATTCTGCGG CTGAAATGAC 781 TCCTCGCCAG TGGTGGCTAG CAGTGAGTGA AGCACTGGCC GAAATGCTGC GTGCTCAGCC 841 ATTCGCCAAG CCGGTGGCGA ATCAGCGACA TGTTAACTAC ATCTCAATGG AGTTTTTGAT 901 TGGTCGCCTG ACGGGCAACA ACCTGTTGAA TCTCGGCTGG TATCAGGATG TACAGGATTC 961 GTTGAAGGCT TATGACATCA ATCTGACGGA CCTGCTGGAA GAAGAGATCG ACCCGGCGCT 1021 GGGTAACGGT GGTCTGGGAC GTCTGGCGGC GTGCTTCCTC GACTCAATGG CAACTGTCGG 1081 TCAGTCTGCG ACGGGTTACG GTCTGAACTA TCAATATGGT TTGTTCCGCC AGTCTTTTGT 1141 CGATGGCAAA CAGGTTGAAG CGCCGGATGA CTGGCATCGC AGTAACTACC CGTGGTTCCG 1201 CCACAACGAA GCACTGGATG TGCAGGTAGG GATTGGCGGT AAAGTGACGA AAGACGGACG 1261 CTGGGAGCCG GAGTTTACCA TTACCGGTCA AGCGTGGGAT CTCCCCGTTG TCGGCTATCG 1321 TAATGGCGTG GCGCAGCCGC TGCGTCTGTG GCAGGCGACG CACGCGCATC CGTTTGATCT 1381 GACTAAATTT AACGACGGTG ATTTCTTGCG TGCCGAACAG CAGGGCATCA ATGCGGAAAA 1441 ACTGACCAAA GTTCTCTATC CAAACGACAA CCATACTGCC GGTAAAAAGC TGCGCCTGAT 1501 GCAGCAATAC TTCCAGTGTG CCTGTTCGGT AGCGGATATT TTGCGTCGCC ATCATCTGGC 1561 GGGGCGTAAA CTGCACGAAC TGGCGGATTA CGAAGTTATT CAGCTGAACG ATACCCACCC 1621 AACTATCGCG ATTCCAGAAC TGCTGCGCGT GCTGATCGAT GAGCACCAGA TGAGCTGGGA 1681 TGACGCCTGG GCCATTACCA GCAAAACTTT CGCTTACACC AACCATACCC TGATGCCAGA 1741 AGCGCTGGAA CGCTGGGATG TGAAACTGGT GAAAGGCTTA CTGCCGCGCC ACATGCAGAT 1801 TATTAACGAA ATTAATACTC GCTTTAAAAC GCTGGTAGAG AAAACCTGGC CGGGCGATGA 1861 AAAAGTGTGG GCCAAACTGG CGGTGGTGCA CGACAAACAA GTGCATATGG CGAACCTGTG 1921 TGTGGTTGGC GGTTTCGCGG TGAACGGTGT TGCGGCGCTG CACTCGGATC TGGTGGTGAA 1981 AGATCTGTTC CCGGAATATC ACCAGCTATG GCCGAACAAA TTCCATAACG TCACCAACGG 2041 TATTACCCCA CGTCGCTGGA TCAAACAGTG CAACCCGGCA CTGGCGGCTC TGTTGGATAA 2101 ATCACTGCAA AAAGAGTGGG CTAACGATCT CGATCAGCTG ATCAATCTGG AAAAATTCGC 2161 TGATGATGCG AAATTCCGTC AGCAATATCG CGAGATCAAG CAGGCGAATA AAGTCCGTCT 2221 GGCGGAGTTT GTGAAAGTTC GTACCGGTAT TGAGATCAAT CCACAGGCGA TTTTCGATAT 2281 TCAGATCAAA CGTTTGCATG AGTACAAACG CCAGCACCTG AATCTGCTGC ATATTCTGGC 2341 GTTGTACAAA GAAATTCGTG AAAACCCGCA GGCTGATCGC GTACCGCGCG TCTTCCTCTT 2401 CGGCGCGAAA GCGGCACCGG GCTACTACCT GGCGAAGAAT ATTATCTTTG CGATCAACAA 2461 AGTGGCTGAC GTGATCAACA ACGATCCGCT GGTTGGCGAT AAGTTGAAGG TGGTGTTCCT 2521 GCCGGATTAT TGCGTTTCGG CGGCGGAAAA ACTGATCCCG GCGGCGGATA TCTCCGAACA 2581 AATTTCGACT GCAGGTAAAG AAGCTTCCGG TACCGGCAAT ATGAAACTGG CGCTCAATGG 2641 TGCGCTTACT GTCGGTACGC TGGATGGGGC GAACGTTGAA ATCGCCGAGA AAGTCGGTGA 2701 AGAAAATATC TTTATTTTTG GTCATACCGT GGAACAAGTG AAGGCAATTC TGGCCAAAGG 2761 CTACGACCCG GTGAAATGGC GGAAGAAAGA TAAGGTGCTG GACGCAGTAT TGAAAGAGCT 2821 GGAAAGCGGT AAATACAGCG ACGGCGATAA GCATGCCTTC GACCAGATGC TGCACAGTAT 2881 CGGCAAACAG GGCGGCGATC CGTATCTGGT GATGGCGGAT TTCGCAGCCT ATGTAGAGGC 2941 ACAAAAGCAG GTGGATGTGC TGTACCGCGA CCAGGAGGCC TGGACTCGCG CGGCGATCCT 3001 CAATACCGCC CGCTGCGGTA TGTTTAGCTC GGATCGCTCT ATTCGCGATT ATCAGGCTCG 3061 TATCTGGCAG GCAAAACGCT AGGACCCAGC TTTCTTGTAC AAAGTTGGCA TTATAAGAAA 3121 GCATTGCTTA TCAATTTGTT GCAACGAACA GGTCACTATC AGTCAAAATA AAATCATTAT 3181 TTGCCATCCA GCTGATATCC CCTATAGTGA GTCGTATTAC ATGGTCATAG CTGTTTCCTG 3241 GCAGCTCTGG CCCGTGTCTC AAAATCTCTG ATGTTACATT GCACAAGATA AAATAATATC 3301 ATCATGAACA ATAAAACTGT CTGCTTACAT AAACAGTAAT ACAAGGGGTG TTATGAGCCA 3361 TATTCAACGG GAAACGTCGA GGCCGCGATT AAATTCCAAC ATGGATGCTG ATTTATATGG 3421 GTATAAATGG GCTCGCGATA ATGTCGGGCA ATCAGGTGCG ACAATCTATC GCTTGTATGG 3481 GAAGCCCGAT GCGCCAGAGT TGTTTCTGAA ACATGGCAAA GGTAGCGTTG CCAATGATGT 3541 TACAGATGAG ATGGTCAGAC TAAACTGGCT GACGGAATTT ATGCCTCTTC CGACCATCAA 3601 GCATTTTATC CGTACTCCTG ATGATGCATG GTTACTCACC ACTGCGATCC CCGGAAAAAC 3661 AGCATTCCAG GTATTAGAAG AATATCCTGA TTCAGGTGAA AATATTGTTG ATGCGCTGGC 3721 AGTGTTCCTG CGCCGGTTGC ATTCGATTCC TGTTTGTAAT TGTCCTTTTA ACAGCGATCG 3781 CGTATTTCGT CTCGCTCAGG CGCAATCACG AATGAATAAC GGTTTGGTTG ATGCGAGTGA 3841 TTTTGATGAC GAGCGTAATG GCTGGCCTGT TGAACAAGTC TGGAAAGAAA TGCATAAACT 3901 TTTGCCATTC TCACCGGATT CAGTCGTCAC TCATGGTGAT TTCTCACTTG ATAACCTTAT 3961 TTTTGACGAG GGGAAATTAA TAGGTTGTAT TGATGTTGGA CGAGTCGGAA TCGCAGACCG 4021 ATACCAGGAT CTTGCCATCC TATGGAACTG CCTCGGTGAG TTTTCTCCTT CATTACAGAA 4081 ACGGCTTTTT CAAAAATATG GTATTGATAA TCCTGATATG AATAAATTGC AGTTTCATTT 4141 GATGCTCGAT GAGTTTTTCT AATCAGAATT GGTTAATTGG TTGTAACACT GGCAGAGCAT 4201 TACGCTGACT TGACGGGACG GCGCAAGCTC ATGACCAAAA TCCCTTAACG TGAGTTACGC 4261 GTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT 4321 TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG 4381 TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA 4441 GATACCAAAT ACTGTTCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT 4501 AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA 4561 TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC 4621 GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT 4681 GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA 4741 CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG 4801 AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT 4861 TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT 4921 ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTT //