LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(1586..1682) /label=attL2 /note="attL2 (c)" misc_signal complement(1697..1716) /note="T7 Promoter/priming site (c)" primer_bind 1724..1740 /note="M13 Reverse priming site" gene 1853..2662 /note="Kanamycin resistance gene" rep_origin 2783..3456 /note="pUC origin" vector join(1594..3459,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 671..691 /note="TEV site" misc_feature 692..1579 /note="BGT27_T18a" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg cAATAATTCA AAATTGACTA AAACATCGAT 721 TATTATCCCC TGTAAAAATG AAGGAATTTA TGTTAAACAG ACTATAGAGT TTTTATATAG 781 AACTGAAGCT AAGTATATAT CAACTATAAT CGTCATTAAT GATAACTCTA ACGATAATTG 841 TTGTGAATTT TTGAAAAATC CTTTTAACAA ATTTTCAAAG GTAAAATTGA TAGAAACTAC 901 TGGCATAGGA GCAGCCGCAG CAAGAAATCT TGGGGCAACC TTTGCAGAAA ACTCAGAAAT 961 ATTAGTTTTC TGTGATGCTC ATATTACAAT GAACCAAAAT TGGCTTAATA TTCTTTTATC 1021 TGCCTTTAAT GATAAAAATG TAAGTGTAAT TTGCCCAGGT ATCGGCCATT TTTCTCCTGC 1081 TAGTCCTGCA GGCTATGGTC AAAGCTTTAA TGAGAGCTTT GAAACATATT GGCTAAAAAA 1141 GCCACTGAAT ATAAAAGAAG TTCCACTAGT TCCTGGTGGT TGCATGGCTA TAAAAAAAGA 1201 AACCTTTGAT GCAGTAGACG GCTTTGATCG AGGTTTCCAT AGTTGGGGCT TTGAAGATGT 1261 AGAACTTTCT ATAAAACTCT GGCTTTTCGG ATATAAAATT TTTGTACATC CTGCTGTTAA 1321 AGTTGGACAC AAATTTAGAA AAATACAACC CTATGATGTT GACCTAACGG AATTCCATTA 1381 CAATAAATTA CGTATGGCAT TTAGTCATTT TAATAAGGAA AGAATCAATA AAATAGTTAG 1441 TTCCCTTCAT AATTGCTCTA ATTTTAAAGC TATTTTAGAT AAAATAACTT TAAGTGATAC 1501 TTATAATCAA CGTATAGCTT ACTTTAAACG TCGTCTATAT GATGATAATT GGTTCTTCGA 1561 TAAATTTAAT ATACCCTTCT AGGACCCAGC TTTCTTGTAC AAAGTTGGCA TTATAAGAAA 1621 GCATTGCTTA TCAATTTGTT GCAACGAACA GGTCACTATC AGTCAAAATA AAATCATTAT 1681 TTGCCATCCA GCTGATATCC CCTATAGTGA GTCGTATTAC ATGGTCATAG CTGTTTCCTG 1741 GCAGCTCTGG CCCGTGTCTC AAAATCTCTG ATGTTACATT GCACAAGATA AAATAATATC 1801 ATCATGAACA ATAAAACTGT CTGCTTACAT AAACAGTAAT ACAAGGGGTG TTATGAGCCA 1861 TATTCAACGG GAAACGTCGA GGCCGCGATT AAATTCCAAC ATGGATGCTG ATTTATATGG 1921 GTATAAATGG GCTCGCGATA ATGTCGGGCA ATCAGGTGCG ACAATCTATC GCTTGTATGG 1981 GAAGCCCGAT GCGCCAGAGT TGTTTCTGAA ACATGGCAAA GGTAGCGTTG CCAATGATGT 2041 TACAGATGAG ATGGTCAGAC TAAACTGGCT GACGGAATTT ATGCCTCTTC CGACCATCAA 2101 GCATTTTATC CGTACTCCTG ATGATGCATG GTTACTCACC ACTGCGATCC CCGGAAAAAC 2161 AGCATTCCAG GTATTAGAAG AATATCCTGA TTCAGGTGAA AATATTGTTG ATGCGCTGGC 2221 AGTGTTCCTG CGCCGGTTGC ATTCGATTCC TGTTTGTAAT TGTCCTTTTA ACAGCGATCG 2281 CGTATTTCGT CTCGCTCAGG CGCAATCACG AATGAATAAC GGTTTGGTTG ATGCGAGTGA 2341 TTTTGATGAC GAGCGTAATG GCTGGCCTGT TGAACAAGTC TGGAAAGAAA TGCATAAACT 2401 TTTGCCATTC TCACCGGATT CAGTCGTCAC TCATGGTGAT TTCTCACTTG ATAACCTTAT 2461 TTTTGACGAG GGGAAATTAA TAGGTTGTAT TGATGTTGGA CGAGTCGGAA TCGCAGACCG 2521 ATACCAGGAT CTTGCCATCC TATGGAACTG CCTCGGTGAG TTTTCTCCTT CATTACAGAA 2581 ACGGCTTTTT CAAAAATATG GTATTGATAA TCCTGATATG AATAAATTGC AGTTTCATTT 2641 GATGCTCGAT GAGTTTTTCT AATCAGAATT GGTTAATTGG TTGTAACACT GGCAGAGCAT 2701 TACGCTGACT TGACGGGACG GCGCAAGCTC ATGACCAAAA TCCCTTAACG TGAGTTACGC 2761 GTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT 2821 TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG 2881 TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA 2941 GATACCAAAT ACTGTTCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT 3001 AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA 3061 TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC 3121 GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT 3181 GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA 3241 CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG 3301 AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT 3361 TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT 3421 ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTT //