LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(1772..1868) /label=attL2 /note="attL2 (c)" misc_signal complement(1883..1902) /note="T7 Promoter/priming site (c)" primer_bind 1910..1926 /note="M13 Reverse priming site" gene 2039..2848 /note="Kanamycin resistance gene" rep_origin 2969..3642 /note="pUC origin" vector join(1780..3645,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 671..691 /note="TEV site" misc_feature 692..1765 /note="BGT21_T01a" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg cGCCTCACAG CTGAAACGGC GCACCACGAT 721 CGCGCGGCCA GTCCGCAAAT CGCCGCCAGT ATCGATCGTC ATACCGTCGC GCGGCGTCGA 781 GCCGTTCACG CAGGAGACGC TGGAGCGCGC CTTCTCACTC GAGTGGCCGC GCTACGAACT 841 GATCTTCTGC GTTGCCCATG GCGACGACCC GGTGGTCAGG TTGATCCGGG CCGCCATTGG 901 CCGCTTTCCA AAAGTGCCGG CCCGGCTGTT GATCGGCGAC GACCGCGTCA GCGCCAATCC 961 CAAGCTCAAC AATTGCGTCA AGGGCTGGGA AGCCGCGCGG CACAACTGGG TCGTCCTGGC 1021 CGACTCCAAC GTGCTGATGC CGAGAGACTA CATCCAGCAT CTGATGGCGG CGTGGCGGCC 1081 GGATACCGGC CTTGTCTGCT CGACGCCGAT CGGCTCGCGG CCGGAGGGCT TCTGGGCCGA 1141 GGTCGAATGC GCCTTCCTCA ACACGCTGCA GGCCCGCTGG CAGTATGCTG GCGAGGCGCT 1201 CGGCCTTGGC TTTGCCCAGG GCAAGAGCAT GCTGTGGAAC AAGCCGATGC TGGATGCCAA 1261 TGGCGGCATC CGCGCGCTGG CCGCTGAAAT CGCCGAGGAC GCCGCCGCAA CCAAGCTGGT 1321 CAATGGGCTT GGCCTGCGAG TCAATCTTGT CGCGGCACCC TTCGAGCAGC CGCTTGGCCG 1381 GCGCACGCTG GACGCGATCT GGTCGCGCCA GGCTCGCTGG GCGCGCCTGC GCCGCGTCAC 1441 CTTCCCGCTG TTCTTCGCGC CGGAAATCCT GACTGGCGCG GTGGTGCCGT TGATGCTTGC 1501 ACTGGTTGCG GCGGCAAGCG CCGGCTTCAG CCTGCCGCTG ACGGCCATCG CCGTGCTGGC 1561 CGCCTTCTAC CTTCCGGAAT GCGCCCTGGC ATGGTCCAAG GGCTGGCACC TGTCGCCGCG 1621 CACGGTCGCG GCGATGATTG CGCGGGACTT GATGGTCCCA GCTATGTGGG CACGCGGCTG 1681 GCTTGGCGGC GCCGTCGACT GGCGCGGCAA TGCGATGACC ATCGGCACCA AGGCGGCCGA 1741 ACTGGAAGAG GCGTCGTCGC GCGCCTAGGA CCCAGCTTTC TTGTACAAAG TTGGCATTAT 1801 AAGAAAGCAT TGCTTATCAA TTTGTTGCAA CGAACAGGTC ACTATCAGTC AAAATAAAAT 1861 CATTATTTGC CATCCAGCTG ATATCCCCTA TAGTGAGTCG TATTACATGG TCATAGCTGT 1921 TTCCTGGCAG CTCTGGCCCG TGTCTCAAAA TCTCTGATGT TACATTGCAC AAGATAAAAT 1981 AATATCATCA TGAACAATAA AACTGTCTGC TTACATAAAC AGTAATACAA GGGGTGTTAT 2041 GAGCCATATT CAACGGGAAA CGTCGAGGCC GCGATTAAAT TCCAACATGG ATGCTGATTT 2101 ATATGGGTAT AAATGGGCTC GCGATAATGT CGGGCAATCA GGTGCGACAA TCTATCGCTT 2161 GTATGGGAAG CCCGATGCGC CAGAGTTGTT TCTGAAACAT GGCAAAGGTA GCGTTGCCAA 2221 TGATGTTACA GATGAGATGG TCAGACTAAA CTGGCTGACG GAATTTATGC CTCTTCCGAC 2281 CATCAAGCAT TTTATCCGTA CTCCTGATGA TGCATGGTTA CTCACCACTG CGATCCCCGG 2341 AAAAACAGCA TTCCAGGTAT TAGAAGAATA TCCTGATTCA GGTGAAAATA TTGTTGATGC 2401 GCTGGCAGTG TTCCTGCGCC GGTTGCATTC GATTCCTGTT TGTAATTGTC CTTTTAACAG 2461 CGATCGCGTA TTTCGTCTCG CTCAGGCGCA ATCACGAATG AATAACGGTT TGGTTGATGC 2521 GAGTGATTTT GATGACGAGC GTAATGGCTG GCCTGTTGAA CAAGTCTGGA AAGAAATGCA 2581 TAAACTTTTG CCATTCTCAC CGGATTCAGT CGTCACTCAT GGTGATTTCT CACTTGATAA 2641 CCTTATTTTT GACGAGGGGA AATTAATAGG TTGTATTGAT GTTGGACGAG TCGGAATCGC 2701 AGACCGATAC CAGGATCTTG CCATCCTATG GAACTGCCTC GGTGAGTTTT CTCCTTCATT 2761 ACAGAAACGG CTTTTTCAAA AATATGGTAT TGATAATCCT GATATGAATA AATTGCAGTT 2821 TCATTTGATG CTCGATGAGT TTTTCTAATC AGAATTGGTT AATTGGTTGT AACACTGGCA 2881 GAGCATTACG CTGACTTGAC GGGACGGCGC AAGCTCATGA CCAAAATCCC TTAACGTGAG 2941 TTACGCGTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA 3001 TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT 3061 GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG 3121 AGCGCAGATA CCAAATACTG TTCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA 3181 CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG 3241 TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA 3301 GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC 3361 CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA 3421 GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC 3481 AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG 3541 TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC 3601 CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTT //