LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(1592..1688) /label=attL2 /note="attL2 (c)" misc_signal complement(1703..1722) /note="T7 Promoter/priming site (c)" primer_bind 1730..1746 /note="M13 Reverse priming site" gene 1859..2668 /note="Kanamycin resistance gene" rep_origin 2789..3462 /note="pUC origin" vector join(1600..3465,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 671..691 /note="TEV site" misc_feature 692..1585 /note="BGT11_T02b" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg cGCTTTTAAA GTGGTGCAAA TTTGCGGGGG 721 GCTTGGGAAT CAAATGTTCC AATACGCTTT CGCTAAAAGT TTGCAAAAAC ACTCTAATAC 781 GCCCGTGCTA TTGGATATCA CTTCTTTTGA TGGGAGCAAT AGGAAAATGC AATTAGAGCT 841 TTTCCCTATT GATTTGCCCT ATGCGAGCGC AAAAGAAATC GCTATAGCTA AAATGCAACA 901 CCTCCCCAAG CTAGTAAGAG ACGCGCTCAA ATACATGGGG TTTGATAGGG TGAGTCAAGA 961 AATCGTTTTT GAATACGAGC CTAAATTATT AAAGCCAAGC CGCTTGACTT ATTTTTATGG 1021 CTATTTTCAA GATCCACGAT ATTTTGATGC TATATCCTCT TTAATCAAGC AAACCTTCAC 1081 CCTACCCCCC CCCCCCGAAA ATGGAAATAA TAAAAAAAAA GAGGAAGAAT ACCACCGCAA 1141 GCTTTCTTTG ATTTTAGCCG CTAAAAACAG CGTATTTGCG CATATAAGAA GAGGGGATTA 1201 TGTGGGGATT GGCTGTCAGC TTGGTATTGA CTATCAAAAA AAGGCTGTTG AGTATATGGC 1261 AAAGCGCGTG CCAAACATGG AGCTTTTTGT ATTTTGTGAA GACTTAAAAT TCACGCAAAA 1321 CCTTGATCTT GGCTACCCTT TTATGGACAT GACCACTAGG GATAAAGACG AAGAGGCGTA 1381 TTGGGACATG CTGCTCATGC AATCTTGCAA GCATGGCATT ATCGCTAACA GCACTTATAG 1441 CTGGTGGGCG GCTTATTTGA TAAACAATCC AGGAAAAATC ATCATTGGCC CCAAACACTG 1501 GCTTTTTGGG CATGAAAACA TCCTTTGTAA GGAATGGGTG AAAATAGAAT CCCATTTTGA 1561 GGTGAAATCC CAAAAGTATA ACGCTTAGGA CCCAGCTTTC TTGTACAAAG TTGGCATTAT 1621 AAGAAAGCAT TGCTTATCAA TTTGTTGCAA CGAACAGGTC ACTATCAGTC AAAATAAAAT 1681 CATTATTTGC CATCCAGCTG ATATCCCCTA TAGTGAGTCG TATTACATGG TCATAGCTGT 1741 TTCCTGGCAG CTCTGGCCCG TGTCTCAAAA TCTCTGATGT TACATTGCAC AAGATAAAAT 1801 AATATCATCA TGAACAATAA AACTGTCTGC TTACATAAAC AGTAATACAA GGGGTGTTAT 1861 GAGCCATATT CAACGGGAAA CGTCGAGGCC GCGATTAAAT TCCAACATGG ATGCTGATTT 1921 ATATGGGTAT AAATGGGCTC GCGATAATGT CGGGCAATCA GGTGCGACAA TCTATCGCTT 1981 GTATGGGAAG CCCGATGCGC CAGAGTTGTT TCTGAAACAT GGCAAAGGTA GCGTTGCCAA 2041 TGATGTTACA GATGAGATGG TCAGACTAAA CTGGCTGACG GAATTTATGC CTCTTCCGAC 2101 CATCAAGCAT TTTATCCGTA CTCCTGATGA TGCATGGTTA CTCACCACTG CGATCCCCGG 2161 AAAAACAGCA TTCCAGGTAT TAGAAGAATA TCCTGATTCA GGTGAAAATA TTGTTGATGC 2221 GCTGGCAGTG TTCCTGCGCC GGTTGCATTC GATTCCTGTT TGTAATTGTC CTTTTAACAG 2281 CGATCGCGTA TTTCGTCTCG CTCAGGCGCA ATCACGAATG AATAACGGTT TGGTTGATGC 2341 GAGTGATTTT GATGACGAGC GTAATGGCTG GCCTGTTGAA CAAGTCTGGA AAGAAATGCA 2401 TAAACTTTTG CCATTCTCAC CGGATTCAGT CGTCACTCAT GGTGATTTCT CACTTGATAA 2461 CCTTATTTTT GACGAGGGGA AATTAATAGG TTGTATTGAT GTTGGACGAG TCGGAATCGC 2521 AGACCGATAC CAGGATCTTG CCATCCTATG GAACTGCCTC GGTGAGTTTT CTCCTTCATT 2581 ACAGAAACGG CTTTTTCAAA AATATGGTAT TGATAATCCT GATATGAATA AATTGCAGTT 2641 TCATTTGATG CTCGATGAGT TTTTCTAATC AGAATTGGTT AATTGGTTGT AACACTGGCA 2701 GAGCATTACG CTGACTTGAC GGGACGGCGC AAGCTCATGA CCAAAATCCC TTAACGTGAG 2761 TTACGCGTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA 2821 TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT 2881 GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG 2941 AGCGCAGATA CCAAATACTG TTCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA 3001 CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG 3061 TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA 3121 GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC 3181 CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA 3241 GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC 3301 AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG 3361 TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC 3421 CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTT //