LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(1895..1991) /label=attL2 /note="attL2 (c)" misc_signal complement(2006..2025) /note="T7 Promoter/priming site (c)" primer_bind 2033..2049 /note="M13 Reverse priming site" gene 2162..2971 /note="Kanamycin resistance gene" rep_origin 3092..3765 /note="pUC origin" vector join(1903..3768,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 671..691 /note="TEV site" misc_feature 692..1888 /note="BGT01_T01a" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg cGCAAACGTA CTCGTAATAA ATTTCCCTGG 721 AGAAGGTCAT ATAAATCCGA CTTTAGCTAT TGTAAGTGAG TTAATTCGGC GAGGGGAGAC 781 AGTTGTTTCG TATTGTATTG AAGATTATAG AAAGAAGATT GAAGCAACAG GTGCACAATT 841 CCGAGTGTTT GAGAATTTCC TCTCTCAAAT TAATATTATG GAGCGAGTAA ATGAAGGTGG 901 GAGTCCTTTG ACGATGCTGT CTCATATGAT GGAAGCATCA GAACGTATTG TTACTCAAAT 961 TGTAGAAGAA ACAAAAGGGG AAAAGTACGA TTATTTGATA TATGATAATC ACTTTCCAGT 1021 AGGACGTATT ATAGCCAATG TTTTAAAGTT ACCTAGTGTT TCTTCTTGTA CAACGTTTGC 1081 TTTTAATCAG TACATTACTT TTAACGATGA ACATGAATCA AGAGAAGTAG ATGAAACGAA 1141 TCCATTGTAT CAATCTTGTT TAGCGGGAAT GGAAAAATGG AACAAACAGT ATGGAATGAA 1201 ATGTAATAGT ATGTATGATA TTATGAACCA TCCTGGTGAT ATTACAATTG TGTATACTTC 1261 AAAGGAATAT CAGCCGCGTT CAGATGTATT CGATGAATCG TATAAGTTTG TTGGCCCATC 1321 AATTGCTACT CGAAAAGAAG TAGGTAGCTT TCCTATGGAA GATTTAAAAG ATGAAAAATT 1381 GATTTTCATT TCTATGGGAA CAGTTTTTAA TGAACAACCT GAGTTATATG AAAAATGTTT 1441 TGAAGCGTTT AAAGATGTAG AAGCGACAGT CGTATTAGTT GTTGGTAAGA AGATAAATAT 1501 AAGTCAATTT GAAAACATTC CGAATAACTT TAAGTTGTAT AATTATGTGC CGCAATTAGA 1561 ACTATTACAG TATGCTGATG TATTCGTAAC ACACGGCGGT ATGAATAGTT CAAGTGAAGC 1621 ACTATATTAC GGTGTCCCGT TAGTTGTAAT TCCGGTAACA GGAGATCAGC CTTTAGTTGC 1681 GAAACGAGTA AATGAAGTAG GGGCTGGAAT AAGGCTTAAT CGCAAAGAAT TAACTTCTGA 1741 AATGTTACGT GAGTCTGTAA AGAAAGTGAT GGATGATGTA ACGTTTAAGG AAAAAAGTCG 1801 TAAAGTTGGA GAGTCACTTC GAAATGCTGG TGGTTATAAT AGGGCAGTTG ATGAAATATT 1861 AAAAATGAAT TCATACTCAA AACTTAAATA GGACCCAGCT TTCTTGTACA AAGTTGGCAT 1921 TATAAGAAAG CATTGCTTAT CAATTTGTTG CAACGAACAG GTCACTATCA GTCAAAATAA 1981 AATCATTATT TGCCATCCAG CTGATATCCC CTATAGTGAG TCGTATTACA TGGTCATAGC 2041 TGTTTCCTGG CAGCTCTGGC CCGTGTCTCA AAATCTCTGA TGTTACATTG CACAAGATAA 2101 AATAATATCA TCATGAACAA TAAAACTGTC TGCTTACATA AACAGTAATA CAAGGGGTGT 2161 TATGAGCCAT ATTCAACGGG AAACGTCGAG GCCGCGATTA AATTCCAACA TGGATGCTGA 2221 TTTATATGGG TATAAATGGG CTCGCGATAA TGTCGGGCAA TCAGGTGCGA CAATCTATCG 2281 CTTGTATGGG AAGCCCGATG CGCCAGAGTT GTTTCTGAAA CATGGCAAAG GTAGCGTTGC 2341 CAATGATGTT ACAGATGAGA TGGTCAGACT AAACTGGCTG ACGGAATTTA TGCCTCTTCC 2401 GACCATCAAG CATTTTATCC GTACTCCTGA TGATGCATGG TTACTCACCA CTGCGATCCC 2461 CGGAAAAACA GCATTCCAGG TATTAGAAGA ATATCCTGAT TCAGGTGAAA ATATTGTTGA 2521 TGCGCTGGCA GTGTTCCTGC GCCGGTTGCA TTCGATTCCT GTTTGTAATT GTCCTTTTAA 2581 CAGCGATCGC GTATTTCGTC TCGCTCAGGC GCAATCACGA ATGAATAACG GTTTGGTTGA 2641 TGCGAGTGAT TTTGATGACG AGCGTAATGG CTGGCCTGTT GAACAAGTCT GGAAAGAAAT 2701 GCATAAACTT TTGCCATTCT CACCGGATTC AGTCGTCACT CATGGTGATT TCTCACTTGA 2761 TAACCTTATT TTTGACGAGG GGAAATTAAT AGGTTGTATT GATGTTGGAC GAGTCGGAAT 2821 CGCAGACCGA TACCAGGATC TTGCCATCCT ATGGAACTGC CTCGGTGAGT TTTCTCCTTC 2881 ATTACAGAAA CGGCTTTTTC AAAAATATGG TATTGATAAT CCTGATATGA ATAAATTGCA 2941 GTTTCATTTG ATGCTCGATG AGTTTTTCTA ATCAGAATTG GTTAATTGGT TGTAACACTG 3001 GCAGAGCATT ACGCTGACTT GACGGGACGG CGCAAGCTCA TGACCAAAAT CCCTTAACGT 3061 GAGTTACGCG TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG 3121 AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC 3181 GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG 3241 CAGAGCGCAG ATACCAAATA CTGTTCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA 3301 GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC 3361 CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC 3421 GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA 3481 CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG 3541 AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT 3601 TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA 3661 GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC 3721 GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTT //