LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(1790..1886) /label=attL2 /note="attL2 (c)" misc_signal complement(1901..1920) /note="T7 Promoter/priming site (c)" primer_bind 1928..1944 /note="M13 Reverse priming site" gene 2057..2866 /note="Kanamycin resistance gene" rep_origin 2987..3660 /note="pUC origin" vector join(1798..3663,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 671..691 /note="TEV site" misc_feature 692..1783 /note="BGH99_B01a" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg cAACGGTAAC AAAAAGAAAA TGAACGGTGA 721 ACACGATTTG GATGCGGCAA ACATTACGTT GGATGACCAT ACGATCAGTT TTTATTATAA 781 TTGGTATGGA AATCCGTCAG TGGATGGAGA AATGAAGCAC TGGATGCACC CGATAGCCCT 841 TGCTCCGGGA CATTCGGGAG ATGTCGGTGC CATATCCGGA CTTAATGATG ACATCGCCTG 901 TAATTTTTAT CCGGAGCTCG GAACGTACAG CAGCAATGAT CCTGAAATCA TTCGGAAACA 961 TATCCGGATG CATATAAAAG CGAATGTCGG TGTACTGTCT GTCACTTGGT GGGGAGAAAG 1021 CGATTATGGC AACCAAAGTG TGTCTCTCCT GCTGGATGAG GCTGCAAAAG TAGGGGCAAA 1081 GGTGTGCTTT CATATAGAGC CTTTTAATGG ACGCAGCCCG CAAACGGTAA GGGAGAATAT 1141 TCAATATATA GTGGATACTT ATGGTGATCA TCCGGCTTTT TACCGTACGC ACGGCAAACC 1201 TCTTTTCTTT ATCTATGATT CTTATCTGAT CAAACCTGCC GAGTGGGCGA AGTTGTTTGC 1261 TGCCGGGGGA GAGATAAGTG TGCGTAATAC CAAGTACGAC GGTCTTTTTA TTGGTCTGAC 1321 ATTGAAGGAA AGCGAGTTGC CCGACATTGA GACAGCGTGC ATGGATGGCT TTTACACTTA 1381 CTTTGCCGCA ACAGGTTTCA CAAATGCTTC TACTCCGGCC AACTGGAAAT CCATGCAGCA 1441 ATGGGCAAAG GCACATAATA AATTGTTTAT TCCGAGTGTC GGTCCGGGAT ATATTGATAC 1501 CCGGATTCGT CCTTGGAACG GAAGTACCAC CCGAGACCGT GAGAATGGAA AATATTACGA 1561 TGATATGTAT AAAGCTGCCA TAGAAAGCGG TGCTTCTTAT ATTTCGATTA CGTCTTTCAA 1621 TGAATGGCAT GAAGGAACTC AGATAGAGCC GGCTGTCTCA AAGAAGTGCG ATGCTTTTGA 1681 ATATTTGGAT TATAAACCAT TGGCTGATGA TTACTATTTG ATAAGAACTG CCTATTGGGT 1741 AGATGAATTC CGGAAAGCAA GATCTGCTTC GGAAGATGTT CAATAGGACC CAGCTTTCTT 1801 GTACAAAGTT GGCATTATAA GAAAGCATTG CTTATCAATT TGTTGCAACG AACAGGTCAC 1861 TATCAGTCAA AATAAAATCA TTATTTGCCA TCCAGCTGAT ATCCCCTATA GTGAGTCGTA 1921 TTACATGGTC ATAGCTGTTT CCTGGCAGCT CTGGCCCGTG TCTCAAAATC TCTGATGTTA 1981 CATTGCACAA GATAAAATAA TATCATCATG AACAATAAAA CTGTCTGCTT ACATAAACAG 2041 TAATACAAGG GGTGTTATGA GCCATATTCA ACGGGAAACG TCGAGGCCGC GATTAAATTC 2101 CAACATGGAT GCTGATTTAT ATGGGTATAA ATGGGCTCGC GATAATGTCG GGCAATCAGG 2161 TGCGACAATC TATCGCTTGT ATGGGAAGCC CGATGCGCCA GAGTTGTTTC TGAAACATGG 2221 CAAAGGTAGC GTTGCCAATG ATGTTACAGA TGAGATGGTC AGACTAAACT GGCTGACGGA 2281 ATTTATGCCT CTTCCGACCA TCAAGCATTT TATCCGTACT CCTGATGATG CATGGTTACT 2341 CACCACTGCG ATCCCCGGAA AAACAGCATT CCAGGTATTA GAAGAATATC CTGATTCAGG 2401 TGAAAATATT GTTGATGCGC TGGCAGTGTT CCTGCGCCGG TTGCATTCGA TTCCTGTTTG 2461 TAATTGTCCT TTTAACAGCG ATCGCGTATT TCGTCTCGCT CAGGCGCAAT CACGAATGAA 2521 TAACGGTTTG GTTGATGCGA GTGATTTTGA TGACGAGCGT AATGGCTGGC CTGTTGAACA 2581 AGTCTGGAAA GAAATGCATA AACTTTTGCC ATTCTCACCG GATTCAGTCG TCACTCATGG 2641 TGATTTCTCA CTTGATAACC TTATTTTTGA CGAGGGGAAA TTAATAGGTT GTATTGATGT 2701 TGGACGAGTC GGAATCGCAG ACCGATACCA GGATCTTGCC ATCCTATGGA ACTGCCTCGG 2761 TGAGTTTTCT CCTTCATTAC AGAAACGGCT TTTTCAAAAA TATGGTATTG ATAATCCTGA 2821 TATGAATAAA TTGCAGTTTC ATTTGATGCT CGATGAGTTT TTCTAATCAG AATTGGTTAA 2881 TTGGTTGTAA CACTGGCAGA GCATTACGCT GACTTGACGG GACGGCGCAA GCTCATGACC 2941 AAAATCCCTT AACGTGAGTT ACGCGTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT 3001 CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA 3061 ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA 3121 GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTT CTTCTAGTGT AGCCGTAGTT 3181 AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT 3241 ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA 3301 GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT 3361 GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC 3421 GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA 3481 GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG 3541 CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA 3601 AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT 3661 GTT //