LOCUS       BGH85_B02b2-pET16-DEST       7822 bp    DNA     circular     17-AUG-2011
FEATURES             Location/Qualifiers
     misc_feature    5323..5352
                     /note="His10-tag"
     misc_feature    5365..5376
                     /note="X-factor"
     misc_feature    5230..5245
                     /note="T7 Promoter"
     misc_feature    5249..5273
                     /note="Lac O"
     misc_feature    7549..7610
                     /note="T7 Terminator"
     misc_recomb     complement(5392..5416)
                     /label=attB1
                     /note="attB1 site"
     misc_recomb     7468..7490
                     /label=attB2
                     /note="attB2 site"
     misc_feature    complement(3761..4720)
                     /note="Lac I"
     misc_feature    209..1069
                     /note="Ap"
     misc_feature    1224..1843
                     /note="Ori"
     misc_feature    5419..5439
                     /note="TEV site"
     misc_feature    5440..7461
                     /note="BGH85_B02b2"
     vector          join(7476..7822,1..5399)
                     /source="pET16-DEST"
                     /type="Destination Vector"
     clone           5400..7475
                     /source="BGH85_B02b2-pDONR221"
                     /type="Entry Clone"
ORIGIN      
          1 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
         61 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
        121 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
        181 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
        241 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
        301 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
        361 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
        421 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg
        481 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
        541 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
        601 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
        661 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
        721 accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact
        781 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
        841 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
        901 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
        961 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
       1021 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
       1081 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
       1141 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
       1201 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
       1261 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
       1321 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
       1381 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
       1441 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
       1501 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
       1561 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
       1621 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
       1681 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
       1741 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
       1801 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
       1861 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
       1921 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
       1981 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
       2041 tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc
       2101 gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc
       2161 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
       2221 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
       2281 cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga
       2341 tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc
       2401 ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg
       2461 tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca
       2521 cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac
       2581 tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg
       2641 ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga
       2701 acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga
       2761 agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc
       2821 gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg
       2881 tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga
       2941 tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg
       3001 tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc
       3061 cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg
       3121 acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt
       3181 ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt
       3241 taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg
       3301 cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat
       3361 aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc
       3421 ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt
       3481 gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat
       3541 cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg
       3601 tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg
       3661 cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gagatcccgg
       3721 tgcctaatga gtgagctaac ttacattaat tgcgttgcgc tcactgcccg ctttccagtc
       3781 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
       3841 gcgtattggg cgccagggtg gtttttcttt tcaccagtga gacgggcaac agctgattgc
       3901 ccttcaccgc ctggccctga gagagttgca gcaagcggtc cacgctggtt tgccccagca
       3961 ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata acatgagctg tcttcggtat
       4021 cgtcgtatcc cactaccgag atatccgcac caacgcgcag cccggactcg gtaatggcgc
       4081 gcattgcgcc cagcgccatc tgatcgttgg caaccagcat cgcagtggga acgatgccct
       4141 cattcagcat ttgcatggtt tgttgaaaac cggacatggc actccagtcg ccttcccgtt
       4201 ccgctatcgg ctgaatttga ttgcgagtga gatatttatg ccagccagcc agacgcagac
       4261 gcgccgagac agaacttaat gggcccgcta acagcgcgat ttgctggtga cccaatgcga
       4321 ccagatgctc cacgcccagt cgcgtaccgt cttcatggga gaaaataata ctgttgatgg
       4381 gtgtctggtc agagacatca agaaataacg ccggaacatt agtgcaggca gcttccacag
       4441 caatggcatc ctggtcatcc agcggatagt taatgatcag cccactgacg cgttgcgcga
       4501 gaagattgtg caccgccgct ttacaggctt cgacgccgct tcgttctacc atcgacacca
       4561 ccacgctggc acccagttga tcggcgcgag atttaatcgc cgcgacaatt tgcgacggcg
       4621 cgtgcagggc cagactggag gtggcaacgc caatcagcaa cgactgtttg cccgccagtt
       4681 gttgtgccac gcggttggga atgtaattca gctccgccat cgccgcttcc actttttccc
       4741 gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc tgataagaga
       4801 caccggcata ctctgcgaca tcgtataacg ttactggttt cacattcacc accctgaatt
       4861 gactctcttc cgggcgctat catgccatac cgcgaaaggt tttgcgccat tcgatggtgt
       4921 ccgggatctc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg
       4981 ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac
       5041 agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg
       5101 aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca
       5161 cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc
       5221 ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag
       5281 aaataatttt gtttaacttt aagaaggaga tataccatgg gccatcatca tcatcatcat
       5341 catcatcatc acagcagcgg ccatatcgaa ggtcgtcata tgctcgaATC AACAAGTTTG
       5401 TACAAAAAAG CAGGCTctga aaacttgtac tttcaaggcA CACCTAAACA AGATACAAGT
       5461 GTTGTAAATT ATGTGAAAAC TCAAGAAGAA ACTTCAAACT CAATTCAGAA TCAACCAATC
       5521 TCATCTTATT GGTATCCAGA AGATTTATTA AAATGGAGTG CTAACAATGA TAAAGATGCA
       5581 AAATTTAATA AAAGCACAGT TCCATTAGCA AAAAGAGTCG AAAAGGATAA ACTTGATACT
       5641 ATTAATGACA CTCAAAATAA AGATGTTAAA GTTGTAGCAA TTTCAATAAT GAATGCTAAT
       5701 ACTAGTGGCA ATCCATCACA AGGTTCAAAT AAATTTAGTG CTAATACATT CTCTTATTGG
       5761 CAATACATAG ATAAATTAGT TTACTGGGGT GGATCAGCTG GAGAAGGATT AATAGTTCCT
       5821 CCAAGTCCAG ATGTTACTGA CTCAGCACAC AGAAATGGGG TTCCTGTTTT AGGTACTGTA
       5881 TTCTTCCCTA TGACAGCTCA TGGTGGAAAA ATGGAATGGT TAAATAAATT CCTTGAAAAA
       5941 GATTCTAATG GAAACTTCCC TATAGTAGAT AAGTTAATTG AAGTTGCAGA AAACTATGGC
       6001 TTTGATGGTT GGTTCATAAA TCAAGAAACA GAAGGAACTG AACAAGAACC TTTAACGCCT
       6061 GAACATGCTA AGCTTATGCA AGAATTAATA AAGCAATTTA AAGCTAAGTC TAATGGAAAA
       6121 TTTGAAATTA TGTGGTATGA TTCCATGACA AAAGATGGGG ATATGGATTG GCAAAATGCT
       6181 TTAACTGATA AAAATGAATA TTTCTTATTA GATGGAGATA AAAACAAAGT TGCTGATAGT
       6241 ATGTTCTTAA ATTTCTGGTG GACATATAAT AGTTTAAAAG ATAAAGATTT ATTAAGAGTA
       6301 TCTAATGAAA AAGCTAAAGA AATAGGAATA AACCCTTATG ACTTATATGC TGGAATAGAT
       6361 GTTCAAGCAA ATGGATATAA AACTCCTCTT AGATGGGCTC ATTATCAAAG AGATGACCAA
       6421 GCTCCATTTA CTTCATTAGG TTTATATTGT CCAAGCTGGA CTTATTTTGA TGCTAAAACT
       6481 CCAGAACAAT TTCAAGAAAA CGAAAGTAGA TTATGGGTTA ATGAACATGG TGATCCATCA
       6541 AAGGCAACTA ATGCTCAAGG AGTTGATTGG AGAGGAATTT CAACATACTC TGTTGAAAAA
       6601 AGTGCTGTAA CTAGCCTTCC ATTTACAACT AACTTTAATA TGGGTAATGG ATATGATTTT
       6661 TATGTTGATG GTAATAAAGT ATCTACTCAA GATTGGAATA ATAGAAGTTT AAATGATATA
       6721 ATGCCAACTT ACAGATGGGT TATTTCAAAT GAAGAAAATA ATAATGTAAA AGCTGACATA
       6781 GATTATACTA ATGCTTACTA TGGTGGTAAC TCAATTAAAC TTTCTGGTTA CCTAGCTGAA
       6841 GGAAAAGCTT CTACAATAAA ACTTTATAGT TCTGATTTGA CTTTACCTGA AGGTGTACAA
       6901 TTCACTACTA CTGCTAAGGC TAATGGAAAT ACTGTAGATA TGGATTTAGT TCTTACATTC
       6961 CATGATGGTA CAGAAACTAC TATTTCTGCT GATAAGAAAC TTGGTACTGA TTGGACTAAA
       7021 CTTACTTATG ATGTTTCACC ATATGTAGGA AAATCAATTA AAACTATTTC TTATAAACTT
       7081 TCAAGCCCAG TATGTGTTGA TAACTTCTCT GCAAACTTAG GTAATATAAC AATAGAAATT
       7141 CCTAACTCAA GTTCAAAGGT AAATATATCT AATTCTAAAT TAAATGATGT TGACTTTAAA
       7201 GATGGAATAT ATGCAGGAGC TAGACTTTCA TGGAGTCCTG AAGGAAATTC TTCTGATGTA
       7261 CATCATTATG AAATTTACAG AGTTATGAAT GATGGAACTA AAGTTTTACT AGGTGCAACT
       7321 CCTAATACAA GTTATTATGT TAGTGATTTA AGAAGAAATG ATAAAGAAAC AAATACTAAT
       7381 TTTGAGGTAA TTGCTGTTAA CAAAAACTAT AACAGAGGAA ATAGTCAAAG TATAAATATG
       7441 GAATGGCCTG CTTACCCTGC TTAGGACCCA GCTTTCTTGT ACAAAGTGGT TGATtcgagg
       7501 atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat
       7561 aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag
       7621 gaactatatc cggatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc
       7681 tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca
       7741 cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat
       7801 gataagctgt caaacatgag aa
//
