LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2720..2816) /label=attL2 /note="attL2 (c)" misc_signal complement(2831..2850) /note="T7 Promoter/priming site (c)" primer_bind 2858..2874 /note="M13 Reverse priming site" gene 2987..3796 /note="Kanamycin resistance gene" rep_origin 3917..4590 /note="pUC origin" vector join(2728..4593,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 671..691 /note="TEV site" misc_feature 692..2713 /note="BGH85_B02b2" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg cACACCTAAA CAAGATACAA GTGTTGTAAA 721 TTATGTGAAA ACTCAAGAAG AAACTTCAAA CTCAATTCAG AATCAACCAA TCTCATCTTA 781 TTGGTATCCA GAAGATTTAT TAAAATGGAG TGCTAACAAT GATAAAGATG CAAAATTTAA 841 TAAAAGCACA GTTCCATTAG CAAAAAGAGT CGAAAAGGAT AAACTTGATA CTATTAATGA 901 CACTCAAAAT AAAGATGTTA AAGTTGTAGC AATTTCAATA ATGAATGCTA ATACTAGTGG 961 CAATCCATCA CAAGGTTCAA ATAAATTTAG TGCTAATACA TTCTCTTATT GGCAATACAT 1021 AGATAAATTA GTTTACTGGG GTGGATCAGC TGGAGAAGGA TTAATAGTTC CTCCAAGTCC 1081 AGATGTTACT GACTCAGCAC ACAGAAATGG GGTTCCTGTT TTAGGTACTG TATTCTTCCC 1141 TATGACAGCT CATGGTGGAA AAATGGAATG GTTAAATAAA TTCCTTGAAA AAGATTCTAA 1201 TGGAAACTTC CCTATAGTAG ATAAGTTAAT TGAAGTTGCA GAAAACTATG GCTTTGATGG 1261 TTGGTTCATA AATCAAGAAA CAGAAGGAAC TGAACAAGAA CCTTTAACGC CTGAACATGC 1321 TAAGCTTATG CAAGAATTAA TAAAGCAATT TAAAGCTAAG TCTAATGGAA AATTTGAAAT 1381 TATGTGGTAT GATTCCATGA CAAAAGATGG GGATATGGAT TGGCAAAATG CTTTAACTGA 1441 TAAAAATGAA TATTTCTTAT TAGATGGAGA TAAAAACAAA GTTGCTGATA GTATGTTCTT 1501 AAATTTCTGG TGGACATATA ATAGTTTAAA AGATAAAGAT TTATTAAGAG TATCTAATGA 1561 AAAAGCTAAA GAAATAGGAA TAAACCCTTA TGACTTATAT GCTGGAATAG ATGTTCAAGC 1621 AAATGGATAT AAAACTCCTC TTAGATGGGC TCATTATCAA AGAGATGACC AAGCTCCATT 1681 TACTTCATTA GGTTTATATT GTCCAAGCTG GACTTATTTT GATGCTAAAA CTCCAGAACA 1741 ATTTCAAGAA AACGAAAGTA GATTATGGGT TAATGAACAT GGTGATCCAT CAAAGGCAAC 1801 TAATGCTCAA GGAGTTGATT GGAGAGGAAT TTCAACATAC TCTGTTGAAA AAAGTGCTGT 1861 AACTAGCCTT CCATTTACAA CTAACTTTAA TATGGGTAAT GGATATGATT TTTATGTTGA 1921 TGGTAATAAA GTATCTACTC AAGATTGGAA TAATAGAAGT TTAAATGATA TAATGCCAAC 1981 TTACAGATGG GTTATTTCAA ATGAAGAAAA TAATAATGTA AAAGCTGACA TAGATTATAC 2041 TAATGCTTAC TATGGTGGTA ACTCAATTAA ACTTTCTGGT TACCTAGCTG AAGGAAAAGC 2101 TTCTACAATA AAACTTTATA GTTCTGATTT GACTTTACCT GAAGGTGTAC AATTCACTAC 2161 TACTGCTAAG GCTAATGGAA ATACTGTAGA TATGGATTTA GTTCTTACAT TCCATGATGG 2221 TACAGAAACT ACTATTTCTG CTGATAAGAA ACTTGGTACT GATTGGACTA AACTTACTTA 2281 TGATGTTTCA CCATATGTAG GAAAATCAAT TAAAACTATT TCTTATAAAC TTTCAAGCCC 2341 AGTATGTGTT GATAACTTCT CTGCAAACTT AGGTAATATA ACAATAGAAA TTCCTAACTC 2401 AAGTTCAAAG GTAAATATAT CTAATTCTAA ATTAAATGAT GTTGACTTTA AAGATGGAAT 2461 ATATGCAGGA GCTAGACTTT CATGGAGTCC TGAAGGAAAT TCTTCTGATG TACATCATTA 2521 TGAAATTTAC AGAGTTATGA ATGATGGAAC TAAAGTTTTA CTAGGTGCAA CTCCTAATAC 2581 AAGTTATTAT GTTAGTGATT TAAGAAGAAA TGATAAAGAA ACAAATACTA ATTTTGAGGT 2641 AATTGCTGTT AACAAAAACT ATAACAGAGG AAATAGTCAA AGTATAAATA TGGAATGGCC 2701 TGCTTACCCT GCTTAGGACC CAGCTTTCTT GTACAAAGTT GGCATTATAA GAAAGCATTG 2761 CTTATCAATT TGTTGCAACG AACAGGTCAC TATCAGTCAA AATAAAATCA TTATTTGCCA 2821 TCCAGCTGAT ATCCCCTATA GTGAGTCGTA TTACATGGTC ATAGCTGTTT CCTGGCAGCT 2881 CTGGCCCGTG TCTCAAAATC TCTGATGTTA CATTGCACAA GATAAAATAA TATCATCATG 2941 AACAATAAAA CTGTCTGCTT ACATAAACAG TAATACAAGG GGTGTTATGA GCCATATTCA 3001 ACGGGAAACG TCGAGGCCGC GATTAAATTC CAACATGGAT GCTGATTTAT ATGGGTATAA 3061 ATGGGCTCGC GATAATGTCG GGCAATCAGG TGCGACAATC TATCGCTTGT ATGGGAAGCC 3121 CGATGCGCCA GAGTTGTTTC TGAAACATGG CAAAGGTAGC GTTGCCAATG ATGTTACAGA 3181 TGAGATGGTC AGACTAAACT GGCTGACGGA ATTTATGCCT CTTCCGACCA TCAAGCATTT 3241 TATCCGTACT CCTGATGATG CATGGTTACT CACCACTGCG ATCCCCGGAA AAACAGCATT 3301 CCAGGTATTA GAAGAATATC CTGATTCAGG TGAAAATATT GTTGATGCGC TGGCAGTGTT 3361 CCTGCGCCGG TTGCATTCGA TTCCTGTTTG TAATTGTCCT TTTAACAGCG ATCGCGTATT 3421 TCGTCTCGCT CAGGCGCAAT CACGAATGAA TAACGGTTTG GTTGATGCGA GTGATTTTGA 3481 TGACGAGCGT AATGGCTGGC CTGTTGAACA AGTCTGGAAA GAAATGCATA AACTTTTGCC 3541 ATTCTCACCG GATTCAGTCG TCACTCATGG TGATTTCTCA CTTGATAACC TTATTTTTGA 3601 CGAGGGGAAA TTAATAGGTT GTATTGATGT TGGACGAGTC GGAATCGCAG ACCGATACCA 3661 GGATCTTGCC ATCCTATGGA ACTGCCTCGG TGAGTTTTCT CCTTCATTAC AGAAACGGCT 3721 TTTTCAAAAA TATGGTATTG ATAATCCTGA TATGAATAAA TTGCAGTTTC ATTTGATGCT 3781 CGATGAGTTT TTCTAATCAG AATTGGTTAA TTGGTTGTAA CACTGGCAGA GCATTACGCT 3841 GACTTGACGG GACGGCGCAA GCTCATGACC AAAATCCCTT AACGTGAGTT ACGCGTCGTT 3901 CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT 3961 GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC 4021 GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC 4081 AAATACTGTT CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC 4141 GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC 4201 GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG 4261 AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA 4321 CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA 4381 TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC 4441 CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG 4501 ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT 4561 CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTT //