LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2480..2576) /label=attL2 /note="attL2 (c)" misc_signal complement(2591..2610) /note="T7 Promoter/priming site (c)" primer_bind 2618..2634 /note="M13 Reverse priming site" gene 2747..3556 /note="Kanamycin resistance gene" rep_origin 3677..4350 /note="pUC origin" vector join(2488..4353,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 671..691 /note="TEV site" misc_feature 692..2473 /note="BGH84_B01c" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg cGTAGGACCT AAAACTGGGG AAGAAAACCA 721 GGTTTTAGTT CCAAATTTAA ATCCAACACC AGAAAATTTA GAGGTTGTAG GAGATGGATT 781 TAAGATAACT TCTTCTATAA ATTTAGTTGG TGAGGAAGAG GCAGATGAGA ATGCAGTAAA 841 TGCTTTAAGG GAATTTTTAA CTGCAAACAA CATAGAAATA AATAGTGAAA ATGATCCAAA 901 TTCAACTACC CTTATAATAG GTGAGGTTGA TGATGATATT CCTGAGTTAG ATGAAGCTTT 961 AAATGGAACT ACAGCAGAAA ATTTAAAAGA AGAGGGATAT GCTTTAGTTT CAAATGATGG 1021 GAAAATAGCT ATTGAGGGTA AAGATGGAGA TGGAACTTTC TATGGAGTTC AAACATTTAA 1081 GCAATTAGTT AAGGAATCTA ATATACCAGA AGTAAATATA ACTGATTATC CAACAGTTAG 1141 TGCTAGAGGT ATTGTAGAAG GCTTTTATGG AACTCCTTGG ACACATCAAG ATAGATTAGA 1201 TCAAATTAAA TTTTATGGTG AAAATAAATT AAACACATAT ATTTATGCTC CTAAAGATGA 1261 TCCATATCAC AGAGAAAAAT GGAGAGAGCC ATATCCAGAA AGTGAAATGC AAAGAATGCA 1321 AGAACTTATA AATGCTTCTG CTGAAAATAA GGTTGATTTT GTTTTCGGTA TTTCTCCAGG 1381 AATAGATATA AGATTTGATG GAGATGCAGG AGAAGAAGAT TTTAATCATT TAATAACAAA 1441 GGCAGAATCT TTATATGACA TGGGTGTAAG AAGTTTTGCA ATCTATTGGG ATGATATTCA 1501 AGATAAGAGT GCAGCTAAAC ATGCTCAAGT TTTAAATAGA TTTAATGAAG AATTTGTAAA 1561 GGCTAAGGGA GATGTAAAAC CATTAATAAC AGTTCCAACA GAGTATGATA CTGGAGCTAT 1621 GGTAAGTAAT GGACAACCTA GAGCTTATAC TAGAATTTTT GCAGAAACTG TTGATCCTAG 1681 TATAGAAGTT ATGTGGACTG GCCCTGGAGT TGTTACAAAT GAAATTCCTT TAAGTGATGC 1741 ACAACTTATA AGTGGAATAT ACAATAGAAA TATGGCAGTA TGGTGGAATT ATCCAGTAAC 1801 AGATTATTTT AAAGGTAAAC TTGCCTTAGG ACCAATGCAT GGATTAGATA AAGGATTAAA 1861 TCAATATGTA GATTTCTTTA CTGTAAACCC AATGGAGCAT GCTGAGCTTT CAAAAATATC 1921 AATACACACA GCTGCTGACT ATAGCTGGAA TATGGATAAC TATGATTATG ATAAGGCTTG 1981 GAATAGAGCA ATTGATATGC TTTATGGTGA TTTAGCAGAA GATATGAAGG TATTTGCAAA 2041 CCATTCAACA AGAATGGACA ATAAAACTTG GGCTAAATCA GGAAGAGAAG ACGCTCCAGA 2101 GCTTAGAGCA AAAATGGATG AGTTATGGAA TAAGTTATCT TCTAAAGAAG ATGCAAGTGC 2161 TCTTATAGAA GAGTTATATG GCGAGTTTGC AAGAATGGAA GAAGCTTGTA ATAATCTAAA 2221 GGCAAATTTA CCTGAAGTTG CTTTAGAAGA ATGCTCAAGA CAACTTGATG AACTTATTAC 2281 TTTAGCTCAA GGGGATAAGG CTTCATTAGA TATGATTGTA GCTCAATTAA ATGAAGATAC 2341 AGAGGCTTAT GAAAGTGCTA AAGAAATTGC ACAAAATAAA TTAAATACAG CCTTAAGTAG 2401 CTTTGCAGTT ATATCTGAAA AGGTTGCTCA ATCATTTATA CAAGAAGCTT TAAGTTTTGA 2461 TTTAACATTA ATATAGGACC CAGCTTTCTT GTACAAAGTT GGCATTATAA GAAAGCATTG 2521 CTTATCAATT TGTTGCAACG AACAGGTCAC TATCAGTCAA AATAAAATCA TTATTTGCCA 2581 TCCAGCTGAT ATCCCCTATA GTGAGTCGTA TTACATGGTC ATAGCTGTTT CCTGGCAGCT 2641 CTGGCCCGTG TCTCAAAATC TCTGATGTTA CATTGCACAA GATAAAATAA TATCATCATG 2701 AACAATAAAA CTGTCTGCTT ACATAAACAG TAATACAAGG GGTGTTATGA GCCATATTCA 2761 ACGGGAAACG TCGAGGCCGC GATTAAATTC CAACATGGAT GCTGATTTAT ATGGGTATAA 2821 ATGGGCTCGC GATAATGTCG GGCAATCAGG TGCGACAATC TATCGCTTGT ATGGGAAGCC 2881 CGATGCGCCA GAGTTGTTTC TGAAACATGG CAAAGGTAGC GTTGCCAATG ATGTTACAGA 2941 TGAGATGGTC AGACTAAACT GGCTGACGGA ATTTATGCCT CTTCCGACCA TCAAGCATTT 3001 TATCCGTACT CCTGATGATG CATGGTTACT CACCACTGCG ATCCCCGGAA AAACAGCATT 3061 CCAGGTATTA GAAGAATATC CTGATTCAGG TGAAAATATT GTTGATGCGC TGGCAGTGTT 3121 CCTGCGCCGG TTGCATTCGA TTCCTGTTTG TAATTGTCCT TTTAACAGCG ATCGCGTATT 3181 TCGTCTCGCT CAGGCGCAAT CACGAATGAA TAACGGTTTG GTTGATGCGA GTGATTTTGA 3241 TGACGAGCGT AATGGCTGGC CTGTTGAACA AGTCTGGAAA GAAATGCATA AACTTTTGCC 3301 ATTCTCACCG GATTCAGTCG TCACTCATGG TGATTTCTCA CTTGATAACC TTATTTTTGA 3361 CGAGGGGAAA TTAATAGGTT GTATTGATGT TGGACGAGTC GGAATCGCAG ACCGATACCA 3421 GGATCTTGCC ATCCTATGGA ACTGCCTCGG TGAGTTTTCT CCTTCATTAC AGAAACGGCT 3481 TTTTCAAAAA TATGGTATTG ATAATCCTGA TATGAATAAA TTGCAGTTTC ATTTGATGCT 3541 CGATGAGTTT TTCTAATCAG AATTGGTTAA TTGGTTGTAA CACTGGCAGA GCATTACGCT 3601 GACTTGACGG GACGGCGCAA GCTCATGACC AAAATCCCTT AACGTGAGTT ACGCGTCGTT 3661 CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT 3721 GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC 3781 GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC 3841 AAATACTGTT CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC 3901 GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC 3961 GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG 4021 AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA 4081 CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA 4141 TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC 4201 CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG 4261 ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT 4321 CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTT //