LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2009..2105) /label=attL2 /note="attL2 (c)" misc_signal complement(2120..2139) /note="T7 Promoter/priming site (c)" primer_bind 2147..2163 /note="M13 Reverse priming site" gene 2276..3085 /note="Kanamycin resistance gene" rep_origin 3206..3879 /note="pUC origin" vector join(2017..3882,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 671..691 /note="TEV site" misc_feature 692..2002 /note="BGH47_B03a" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg cCAAGCGGAG TTATCGCTAA GCGTCAAACA 721 GGAATTTATC AGATCCTGGG ATGCTTACAA ACGATACGCC TGGGGCCATG ATGTATTATT 781 GCCGCAAACG AAAACCTACA CGGACTGGTA CGAACAGCCG ATACGTATTT CGCAGATTGA 841 TGCATATAGC ACCATGAAAG TGATGGGCCT CCAGAAACAG GCCAGAGACA TTGAGAAGTT 901 TGTAACAGAT AGTTGCAACT TTGACATAGA CGTTTACGTA AAGACCTTTG ATGTCAATAA 961 TCGCGTACTG GGAGGACTCT TATACATGTA CTCCTGCACA CACCAGCCTA AAATACTGGA 1021 ACGCGCAAGA GACTTTGGAG ATCGCCTCTT AAAAGCTTTC AGATCACCGA CAGGAATCCC 1081 CTATTACTGG GTCAATCTGA AAACCGGTAA AGTGAAAGGC GAAAAGGTCA ATACCGCAGA 1141 AGCAGCCGCT TATACACTTG AAATGGGCAT CCTGAGTTAT TACACAAAGG ACCCGAAATA 1201 TTATCAGGCG GCGAGGAAAG CGACAGAAGC CATATACGGC AGACGCTCAG CCCTCAACCT 1261 TACCGGCAAC ATCATCAACA ATGAAACCGG TGACTGGCTG GAAACCATCA GTTGTATCGG 1321 CGCAGGAGGC AGCGCTTACT ATGAAAGTAT GCTGAAAACA TGGCTCCTGT TTAAAGATGA 1381 AGCACTGAAA GAAATGTGGG AGAAAACCAT TACAGCCGTA CATCAGTATA ACGCAGAAGA 1441 AAGTGACACC GCAATATGGT ACCGCAGAAC GGACATGTAT TCAGGGGAAA CAAAGAATAC 1501 AACTGCGACA GGTTACGACG CATTCCTGCC AGCTCTTTTA TGTATCGATA ATGATGCAGA 1561 ACAGGCAGCC AGGGTACACC GGACCTGGAA TAATCTCTGG AAACAGTATG GGATGATTCC 1621 GATGATCTAC GACTTCCGCC GGCAGCATAT CACGGCAGCA GCGTACGACC TGAATCCCGA 1681 GATCATCGAG TCAGCCTGGT ACCTGTATGA TTACACAAAA GATACCGCCT ATCAGCAGAT 1741 GGGCAGACAG TACTACAGCG ACATATTGCA GTATTGCAGG GCAGGCGCCG CATTCACTGG 1801 CGTAATGGAT ATCAGAAGTA AACAGCAGCA GAATAAAATG CCGGTCTATT TCTTTGCAAC 1861 AACAATGAAA TACCTGTACC TGCTCTTTAC ACCAGACAGT GGATTGAATA CGCTTGATTA 1921 CGTATTCACA GCAGAAGGAA ACCCGTTTAA AACGGATGAA TTCAAAGCGG CAGAAACAGC 1981 CAGACGTCTG GGCATTAATC AGTAGGACCC AGCTTTCTTG TACAAAGTTG GCATTATAAG 2041 AAAGCATTGC TTATCAATTT GTTGCAACGA ACAGGTCACT ATCAGTCAAA ATAAAATCAT 2101 TATTTGCCAT CCAGCTGATA TCCCCTATAG TGAGTCGTAT TACATGGTCA TAGCTGTTTC 2161 CTGGCAGCTC TGGCCCGTGT CTCAAAATCT CTGATGTTAC ATTGCACAAG ATAAAATAAT 2221 ATCATCATGA ACAATAAAAC TGTCTGCTTA CATAAACAGT AATACAAGGG GTGTTATGAG 2281 CCATATTCAA CGGGAAACGT CGAGGCCGCG ATTAAATTCC AACATGGATG CTGATTTATA 2341 TGGGTATAAA TGGGCTCGCG ATAATGTCGG GCAATCAGGT GCGACAATCT ATCGCTTGTA 2401 TGGGAAGCCC GATGCGCCAG AGTTGTTTCT GAAACATGGC AAAGGTAGCG TTGCCAATGA 2461 TGTTACAGAT GAGATGGTCA GACTAAACTG GCTGACGGAA TTTATGCCTC TTCCGACCAT 2521 CAAGCATTTT ATCCGTACTC CTGATGATGC ATGGTTACTC ACCACTGCGA TCCCCGGAAA 2581 AACAGCATTC CAGGTATTAG AAGAATATCC TGATTCAGGT GAAAATATTG TTGATGCGCT 2641 GGCAGTGTTC CTGCGCCGGT TGCATTCGAT TCCTGTTTGT AATTGTCCTT TTAACAGCGA 2701 TCGCGTATTT CGTCTCGCTC AGGCGCAATC ACGAATGAAT AACGGTTTGG TTGATGCGAG 2761 TGATTTTGAT GACGAGCGTA ATGGCTGGCC TGTTGAACAA GTCTGGAAAG AAATGCATAA 2821 ACTTTTGCCA TTCTCACCGG ATTCAGTCGT CACTCATGGT GATTTCTCAC TTGATAACCT 2881 TATTTTTGAC GAGGGGAAAT TAATAGGTTG TATTGATGTT GGACGAGTCG GAATCGCAGA 2941 CCGATACCAG GATCTTGCCA TCCTATGGAA CTGCCTCGGT GAGTTTTCTC CTTCATTACA 3001 GAAACGGCTT TTTCAAAAAT ATGGTATTGA TAATCCTGAT ATGAATAAAT TGCAGTTTCA 3061 TTTGATGCTC GATGAGTTTT TCTAATCAGA ATTGGTTAAT TGGTTGTAAC ACTGGCAGAG 3121 CATTACGCTG ACTTGACGGG ACGGCGCAAG CTCATGACCA AAATCCCTTA ACGTGAGTTA 3181 CGCGTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC 3241 TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT 3301 TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC 3361 GCAGATACCA AATACTGTTC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC 3421 TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG 3481 CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG 3541 GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA 3601 ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC 3661 GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG 3721 GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG 3781 ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT 3841 TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TT //