LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2555..2651) /label=attL2 /note="attL2 (c)" misc_signal complement(2666..2685) /note="T7 Promoter/priming site (c)" primer_bind 2693..2709 /note="M13 Reverse priming site" gene 2822..3631 /note="Kanamycin resistance gene" rep_origin 3752..4425 /note="pUC origin" vector join(2563..4428,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 671..691 /note="TEV site" misc_feature 692..2548 /note="BGH39_B01a" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg cCGTGAACGT ACGCGAATAA GCGTTGATGC 721 CAGTGAAGCG GTACGCCCGT TCAACCGGTT CTGGCGCGGC ACCGGCTTTT CGCCGGCCGA 781 GCTGCTGCTC GAGCCCGAGA TGCGCCAGAT GCTTGCCTAT ATCGGTGGCC TGCCGAATGA 841 GGGCATCAAG TTCCTGCGGG TGCATTATCT CTACAATCTG CTGAGCGCGA AGGGCGAGGT 901 CGGCTACGAC TGGTCGCTGC TCGACCGCGC GCTCGACGTG ATGATCGAAC ACCGCCTGAA 961 GCCGTTCTTC GAACTGATGG GCAACCCATC CGGCCTGTTC ACCGACTATG AGGACATGGA 1021 TCAGGTCAGG CGCTGGCGGG ATCTCGTGAC GGCAACGGTC GACCGTTATG GCGCGCGTTA 1081 CGGCATGGAC GAATTGCGCA CATGGTATTT CGAAACGACG AACGAGGCCG ACTCCGGCTG 1141 GTGGACCTAC GGGATCAAGG GTTACACCAA CTACTACGAC GCCTGCGTCG CGGGGCTCGA 1201 TGCCATCGAT CCCACCTTGC CGATGGGCGG ACCCGGAACC GCCCGCACGC TCTCGCCGAT 1261 CTTTCGCGCC CTGATGGCCC ATTGCGACAG CGGCACGAGC TGCCTCAGCG ATGACGGCCC 1321 GCCGCGCATC GATTACATCT CGATCCACGA AAAGGGCGTC AACGGCAGCA AGGAAGACCT 1381 GACCCCGAAA ACCAACGGTA TCGTCGACCG CACTTTGCTC GTCGTCGACT ATCTGAAGGA 1441 GCATCACCCG CGCCTGGCGG GCTTGCCCAT CGTCAACGAT GAATGCGATC CGCAGCTTGG 1501 CTGGAGCGAT CATCATAGCT GGCACGGCAA AGCCTATTAC GCCGGTATCA TCGCCCGCAT 1561 CATCGAACAG CACGACCGGC GCATCATCGC CCCGAAAGCG GCGAATTTCA CCTTCCTCAG 1621 CAATGACCAC GCCTTTATCG GCGGCTGGAG CCAGCGCACG ATCTTTGCCT ATTTCGGTTC 1681 GCGCAATTTC ACCAAGGCGC AGTGGGAGCA CAAGACCAAT CTGGATATGC TGGTCACCGA 1741 TGTCGACAGG GCGCCGCCCT TCGACATCAT CAAGAAGCCC GGCCTCACAT CGATGGAACT 1801 GCTGGCAACG CTTGGCGATA CCGTTTGCAA GGTGACGGCC GAACCGCCGC TTGCGCCCGA 1861 CCAGGATGGC CTGGCGATCC TGCCGACGCG GCTGCCGGGC GGCGGTGTTT CCATCAGCCT 1921 CATCCATAGC GTCGATGCCA TCAACCGCTC GGGCCGGACC GCCGTCCGTC TCGAGGTGAG 1981 CGGCTTGGTT CCCGGCCGCC ACGCGCTTTG CCTGCTGCGC ATCGACGAGG AATTCACCAA 2041 TCCGATGGAG GTCTGGGAAG CCCAGCACGA TGAAAGCAAC CCGCGTGGGC TCTTCGAGCC 2101 GGTCGGCGCA CCGCCTGCAC CTGATGAAGC GCAGTTTGCC GAAATGCGCC GCGCGCAGGA 2161 GCCCGCCCTG CTGCATCCGA TCAGCGTCGT CGCGTGCGAC GAAGGCCGGA TCAGTGTCGA 2221 CCTGGATGTG CCATTGCCGT CGCTGACGCA GGTGCTTGTC GTTCCCGATA TCGGCGTGCC 2281 ACCGGCGGCA CCGAGCGGCC TTGTCGTCGA ACGCTATCTC GGCCTCGGCG GTCGGGAGGA 2341 GCGCATGCTG TTCTGGGCTG CCGGCGATGC CAGCCCGGCC ATCTTCTACG ATGTCCTGGT 2401 CAGCACCGAT GGCGCGGCTT TTGAAAAGGT CAGTTCAGCG CCGCTGATCT CGACGGCATT 2461 CCTGCACATG TCGCCGCCGC AGGGCGTGCG TTATGCGGTG CGCGCCCGCG ATGCATTCGG 2521 TCGCCGCAGC GAGCTTTGTC TCTCCCGCTA GGACCCAGCT TTCTTGTACA AAGTTGGCAT 2581 TATAAGAAAG CATTGCTTAT CAATTTGTTG CAACGAACAG GTCACTATCA GTCAAAATAA 2641 AATCATTATT TGCCATCCAG CTGATATCCC CTATAGTGAG TCGTATTACA TGGTCATAGC 2701 TGTTTCCTGG CAGCTCTGGC CCGTGTCTCA AAATCTCTGA TGTTACATTG CACAAGATAA 2761 AATAATATCA TCATGAACAA TAAAACTGTC TGCTTACATA AACAGTAATA CAAGGGGTGT 2821 TATGAGCCAT ATTCAACGGG AAACGTCGAG GCCGCGATTA AATTCCAACA TGGATGCTGA 2881 TTTATATGGG TATAAATGGG CTCGCGATAA TGTCGGGCAA TCAGGTGCGA CAATCTATCG 2941 CTTGTATGGG AAGCCCGATG CGCCAGAGTT GTTTCTGAAA CATGGCAAAG GTAGCGTTGC 3001 CAATGATGTT ACAGATGAGA TGGTCAGACT AAACTGGCTG ACGGAATTTA TGCCTCTTCC 3061 GACCATCAAG CATTTTATCC GTACTCCTGA TGATGCATGG TTACTCACCA CTGCGATCCC 3121 CGGAAAAACA GCATTCCAGG TATTAGAAGA ATATCCTGAT TCAGGTGAAA ATATTGTTGA 3181 TGCGCTGGCA GTGTTCCTGC GCCGGTTGCA TTCGATTCCT GTTTGTAATT GTCCTTTTAA 3241 CAGCGATCGC GTATTTCGTC TCGCTCAGGC GCAATCACGA ATGAATAACG GTTTGGTTGA 3301 TGCGAGTGAT TTTGATGACG AGCGTAATGG CTGGCCTGTT GAACAAGTCT GGAAAGAAAT 3361 GCATAAACTT TTGCCATTCT CACCGGATTC AGTCGTCACT CATGGTGATT TCTCACTTGA 3421 TAACCTTATT TTTGACGAGG GGAAATTAAT AGGTTGTATT GATGTTGGAC GAGTCGGAAT 3481 CGCAGACCGA TACCAGGATC TTGCCATCCT ATGGAACTGC CTCGGTGAGT TTTCTCCTTC 3541 ATTACAGAAA CGGCTTTTTC AAAAATATGG TATTGATAAT CCTGATATGA ATAAATTGCA 3601 GTTTCATTTG ATGCTCGATG AGTTTTTCTA ATCAGAATTG GTTAATTGGT TGTAACACTG 3661 GCAGAGCATT ACGCTGACTT GACGGGACGG CGCAAGCTCA TGACCAAAAT CCCTTAACGT 3721 GAGTTACGCG TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG 3781 AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC 3841 GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG 3901 CAGAGCGCAG ATACCAAATA CTGTTCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA 3961 GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC 4021 CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC 4081 GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA 4141 CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG 4201 AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT 4261 TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA 4321 GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC 4381 GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTT //