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FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2216..2312) /label=attL2 /note="attL2 (c)" misc_signal complement(2327..2346) /note="T7 Promoter/priming site (c)" primer_bind 2354..2370 /note="M13 Reverse priming site" gene 2483..3292 /note="Kanamycin resistance gene" rep_origin 3413..4086 /note="pUC origin" vector join(2224..4089,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 671..691 /note="TEV site" misc_feature 692..2209 /note="BGH37_B01a" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg cGGAACAGCG GTACGGATAG ATTATGCAAG 721 CGGGTTAACT GATCGCGAAA ACTCTATGTT CAAAGAAATC CAGTTGTCAG GCGTTTTTGC 781 CGATTCAAAA ACCTTTGTGG ATAGCCATCC CAAATTGCCC CTGGCGGAAA TCGCCGAGCT 841 TTACCATGTC CGGCAACAGC AGGCGGGTTT TGACCTCGCC GCTTTTGTTC ACCGGTATTT 901 TGAGCTGCCG CCGAGCATTG CCTCCGGTTT TGTCAGCGAT ACCTCGCGCC CGGTGGAAAA 961 GCATATCGAC ATTCTCTGGG ATGTGCTCAC CCGCCAACCG GACAGGCAGG AGGCGGGAAC 1021 CCTGCTGCCC TTACCTTACC CCTATGTCGT TCCCGGCGGC CGCTTCCGCG AAATTTACTA 1081 CTGGGACAGC TATTTCACCA TGCTCGGTTT GCAGGCATCG AAGCGCTGGG ATCTGATGGA 1141 GGGTATGGTG AATAATTTTT CACACCTGAT CGACACCATC GGCTTTATTC CCAACGGCAA 1201 TCGCACCTAT TACGAGGGCC GCTCCCAGCC GCCTTTTTAC GCCCTGATGG TGGAGTTGCT 1261 GGCCAATAAA CAGGGTGAGT CGGTGCTGCT CGCGCATTTG CCGCATTTGC GCAGGGAATA 1321 TGAATTCTGG ATGGAGGGCG CCGCTAAACT TTCGCCCGCT GCACCCGCGC ATCGCCGTGT 1381 GGTGCTGCTG CCGGATGGCA GCATACTCAA TCGCTACTGG GATGATATAG CCGCGCCGCG 1441 CCCGGAATCC TTCCGCGAAG ACTACGAACT GGCGGAAGCC ATCGGCGGCA ACAAGCGCGA 1501 GCTGTACCGC CATATTCGCG CGGCGGCAGA ATCCGGCTGG GACTTCAGCA GCCGCTGGTT 1561 CAAAGATGGC AATGGCATGG CCAGCATCCA CACCACCGAT ATTATCCCGG TGGATTTGAA 1621 TGCGCTGGTC TTTAACCTGG AGCGGATGCT GGCCCATATT TATGGCTTGC AGGGCGACCA 1681 GGATCAGGCC ACGCATTACT ACCAATTGGC GGAGCAGCGC AAACAGGCGT TGCTGCGCTA 1741 CTGTTGGAAT GCGCAGCAGG GATTTTTCCA CGATTACGAT TATGTCGCCG CACAACAGAC 1801 GCCGGTCATG TCGCTGGCGG CGGTTTACCC GCTTTATTTC AGTATGGTCG ACCAGCGCAC 1861 GGGCGACCGG GTCGCCGAAC AGATAGAGGC GCATTTTATC CAGGCGGGCG GTGTGACCAC 1921 GACCCTGGCG ACCACAGGCC AGCAGTGGGA CGCGCCCAAT GGCTGGGCGC CGCTGCAATG 1981 GCTGACCATC CAGGGCCTGC GCAATTATCA CCACAATTCA GCGGCGGAGC AGATCAAACA 2041 GCGCTGGATT GCACTCAACC AGCGCGTTTA CCGCAACACC GGAAAGTTGG TGGAAAAATA 2101 CAACGTCTAT GACCTGGATG TGGCCGGCGG CGGTGGCGAA TATGAATTAC AGGATGGCTT 2161 CGGTTGGACC AACGGTGTCT TGTTGCACTT ACTCAACGAA AGTACACCCT AGGACCCAGC 2221 TTTCTTGTAC AAAGTTGGCA TTATAAGAAA GCATTGCTTA TCAATTTGTT GCAACGAACA 2281 GGTCACTATC AGTCAAAATA AAATCATTAT TTGCCATCCA GCTGATATCC CCTATAGTGA 2341 GTCGTATTAC ATGGTCATAG CTGTTTCCTG GCAGCTCTGG CCCGTGTCTC AAAATCTCTG 2401 ATGTTACATT GCACAAGATA AAATAATATC ATCATGAACA ATAAAACTGT CTGCTTACAT 2461 AAACAGTAAT ACAAGGGGTG TTATGAGCCA TATTCAACGG GAAACGTCGA GGCCGCGATT 2521 AAATTCCAAC ATGGATGCTG ATTTATATGG GTATAAATGG GCTCGCGATA ATGTCGGGCA 2581 ATCAGGTGCG ACAATCTATC GCTTGTATGG GAAGCCCGAT GCGCCAGAGT TGTTTCTGAA 2641 ACATGGCAAA GGTAGCGTTG CCAATGATGT TACAGATGAG ATGGTCAGAC TAAACTGGCT 2701 GACGGAATTT ATGCCTCTTC CGACCATCAA GCATTTTATC CGTACTCCTG ATGATGCATG 2761 GTTACTCACC ACTGCGATCC CCGGAAAAAC AGCATTCCAG GTATTAGAAG AATATCCTGA 2821 TTCAGGTGAA AATATTGTTG ATGCGCTGGC AGTGTTCCTG CGCCGGTTGC ATTCGATTCC 2881 TGTTTGTAAT TGTCCTTTTA ACAGCGATCG CGTATTTCGT CTCGCTCAGG CGCAATCACG 2941 AATGAATAAC GGTTTGGTTG ATGCGAGTGA TTTTGATGAC GAGCGTAATG GCTGGCCTGT 3001 TGAACAAGTC TGGAAAGAAA TGCATAAACT TTTGCCATTC TCACCGGATT CAGTCGTCAC 3061 TCATGGTGAT TTCTCACTTG ATAACCTTAT TTTTGACGAG GGGAAATTAA TAGGTTGTAT 3121 TGATGTTGGA CGAGTCGGAA TCGCAGACCG ATACCAGGAT CTTGCCATCC TATGGAACTG 3181 CCTCGGTGAG TTTTCTCCTT CATTACAGAA ACGGCTTTTT CAAAAATATG GTATTGATAA 3241 TCCTGATATG AATAAATTGC AGTTTCATTT GATGCTCGAT GAGTTTTTCT AATCAGAATT 3301 GGTTAATTGG TTGTAACACT GGCAGAGCAT TACGCTGACT TGACGGGACG GCGCAAGCTC 3361 ATGACCAAAA TCCCTTAACG TGAGTTACGC GTCGTTCCAC TGAGCGTCAG ACCCCGTAGA 3421 AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC 3481 AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT 3541 TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTTCTTC TAGTGTAGCC 3601 GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT 3661 CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG 3721 ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC 3781 CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG 3841 CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC 3901 AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG 3961 GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT 4021 ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC 4081 TCACATGTT //