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FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(1814..1910) /label=attL2 /note="attL2 (c)" misc_signal complement(1925..1944) /note="T7 Promoter/priming site (c)" primer_bind 1952..1968 /note="M13 Reverse priming site" gene 2081..2890 /note="Kanamycin resistance gene" rep_origin 3011..3684 /note="pUC origin" vector join(1822..3687,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 671..691 /note="TEV site" misc_feature 692..1807 /note="BGH33_B04c" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg cGCCGGGGCA CCCGTCCCGC CCGGCGGCGA 721 GCCGCTCTAC ACGGAGCAGG ACCTCGCCGT GAACGGCAGG GAGGGCTTTC CGAACTACCG 781 CATCCCAGCG CTGACCGTCA CGCCCGACGG GGACCTGCTG GCCTCGTACG ACGGCCGCCC 841 GACCGGTATC GACGCGCCCG GCCCCAACTC CATCCTCCAA CGCCGCAGCA CCGACGGCGG 901 CCGGACGTGG GGCGAGCAAC AGGTCGTCAG CGCCGGCCAG ACCACCGCGC CGATCAAGGG 961 GTTCTCCGAC CCCAGCTACC TTGTCGACCG GGAAACCGGG ACCATCTTCA ACTTCCACGT 1021 CTACTCCCAG CGGCAGGGCT TCGCCGGCAG CCGGCCCGGC ACCGACCCGG CAGACCCCAA 1081 CGTGCTCCAC GCCAACGTCG CGACCTCGAC CGACGGCGGT CTGACCTGGT CGCACCGGAC 1141 CATCACGGCC GACATCACCC CGGATCCGGG CTGGCGCAGC CGCTTCGCCG CCTCCGGCGA 1201 AGGCATCCAG CTCCGCTATG GACCCCACGC CGGTCGACTC ATCCAGCAGT ACACGATCAT 1261 CAACGCTGCC GGCGCCTTCC AGGCGGTGAG CGTGTACAGC GACGACCACG GAAGGACCTG 1321 GCGCGCCGGC GAAGCCGTCG GGGTCGGCAT GGACGAGAAC AAGACCGTGG AACTCTCCGA 1381 TGGCCGGGTC CTGCTCAACA GCCGCGACTC GGCCCGCAGC GGATACCGTA AGGTGGCCGT 1441 CTCCACTGAC GGCGGCCACA GCTACGGCCC GGTGACCATC GACCGCGACC TCCCCGACCC 1501 GACGAACAAC GCATCGATCA TCCGGGCCTT CCCTGACGCC CCGGCCGGCT CCGCGCGGGC 1561 CAAGGTCCTG CTCTTCTCCA ACGCCGCCAG CCAGACCTCG CGCAGTCAGG GCACCATCCG 1621 GATGTCCTGC GACGATGGCC AGACCTGGCC GGTTTCGAAG GTCTTCCAGC CCGGCTCGAT 1681 GTCGTACTCC ACCCTGACCG CACTGCCCGA CGGCACCTAC GGGCTGCTGT ACGAGCCGGG 1741 CACCGGCATC AGATACGCCA ACTTCAACCT CGCCTGGCTG GGCGGCATCT GCGCGCCCTT 1801 CACGATTTAG GACCCAGCTT TCTTGTACAA AGTTGGCATT ATAAGAAAGC ATTGCTTATC 1861 AATTTGTTGC AACGAACAGG TCACTATCAG TCAAAATAAA ATCATTATTT GCCATCCAGC 1921 TGATATCCCC TATAGTGAGT CGTATTACAT GGTCATAGCT GTTTCCTGGC AGCTCTGGCC 1981 CGTGTCTCAA AATCTCTGAT GTTACATTGC ACAAGATAAA ATAATATCAT CATGAACAAT 2041 AAAACTGTCT GCTTACATAA ACAGTAATAC AAGGGGTGTT ATGAGCCATA TTCAACGGGA 2101 AACGTCGAGG CCGCGATTAA ATTCCAACAT GGATGCTGAT TTATATGGGT ATAAATGGGC 2161 TCGCGATAAT GTCGGGCAAT CAGGTGCGAC AATCTATCGC TTGTATGGGA AGCCCGATGC 2221 GCCAGAGTTG TTTCTGAAAC ATGGCAAAGG TAGCGTTGCC AATGATGTTA CAGATGAGAT 2281 GGTCAGACTA AACTGGCTGA CGGAATTTAT GCCTCTTCCG ACCATCAAGC ATTTTATCCG 2341 TACTCCTGAT GATGCATGGT TACTCACCAC TGCGATCCCC GGAAAAACAG CATTCCAGGT 2401 ATTAGAAGAA TATCCTGATT CAGGTGAAAA TATTGTTGAT GCGCTGGCAG TGTTCCTGCG 2461 CCGGTTGCAT TCGATTCCTG TTTGTAATTG TCCTTTTAAC AGCGATCGCG TATTTCGTCT 2521 CGCTCAGGCG CAATCACGAA TGAATAACGG TTTGGTTGAT GCGAGTGATT TTGATGACGA 2581 GCGTAATGGC TGGCCTGTTG AACAAGTCTG GAAAGAAATG CATAAACTTT TGCCATTCTC 2641 ACCGGATTCA GTCGTCACTC ATGGTGATTT CTCACTTGAT AACCTTATTT TTGACGAGGG 2701 GAAATTAATA GGTTGTATTG ATGTTGGACG AGTCGGAATC GCAGACCGAT ACCAGGATCT 2761 TGCCATCCTA TGGAACTGCC TCGGTGAGTT TTCTCCTTCA TTACAGAAAC GGCTTTTTCA 2821 AAAATATGGT ATTGATAATC CTGATATGAA TAAATTGCAG TTTCATTTGA TGCTCGATGA 2881 GTTTTTCTAA TCAGAATTGG TTAATTGGTT GTAACACTGG CAGAGCATTA CGCTGACTTG 2941 ACGGGACGGC GCAAGCTCAT GACCAAAATC CCTTAACGTG AGTTACGCGT CGTTCCACTG 3001 AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT 3061 AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA 3121 AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC 3181 TGTTCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC 3241 ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT 3301 TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG 3361 GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA 3421 GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT 3481 AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA 3541 TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC 3601 GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC 3661 CTTTTGCTGG CCTTTTGCTC ACATGTT //