LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2543..2639) /label=attL2 /note="attL2 (c)" misc_signal complement(2654..2673) /note="T7 Promoter/priming site (c)" primer_bind 2681..2697 /note="M13 Reverse priming site" gene 2810..3619 /note="Kanamycin resistance gene" rep_origin 3740..4413 /note="pUC origin" vector join(2551..4416,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 671..691 /note="TEV site" misc_feature 692..2536 /note="BGH31_B04a" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg cGCATACACC CTCGAATCAA CAGGCTTATT 721 GGTATTTCAA CGTGATGACG AAGAAGTTTT GACGATTCAG CCCTACTTGC AGGCTTATGG 781 CTCGCAGTTT GCCGCATTAG GTGTACAAAC CAAGCCTGAT ACGGTGCAAT ACACTAGTTT 841 CGATAACCAG ACCTTTGAGC TTAATCTTGA AACGACGGCA ACCGGCTATC AGTTGGTGTT 901 TCACGTGAAA CATCAGGTTG CTCAAATTGG TTTAGCAATC GGCGTACAAC CAGCAGGCTC 961 GTGGTATGGT ATGGGCGAAC GGGTGATTCA AAGTTGGCCG CTCAACTTGG CTGGTGTGCA 1021 AAGCCAACCA TTTATGACCT ATGATCACGC CAATGATGGC ACACTCAATA TTGTTACGCC 1081 AGCCTGGATT GGCGCGAATG GCGTGGCTTT TATCGTGGCC GAAGATACAG GCCCGTTGCA 1141 TGTCACAATC GATAGCGATC CAGCAGGTGT GATTCGGCTG GTGCAGTTTC CTTCGCCAAC 1201 CCCATTTGGC GCGGGCTTGG ATGGCAGCGA AACCCATTAT GAAGGCACGC GCTTGGTGCT 1261 CGATCTACTG ATTGCTGAAA ATGTCAGCGT TGCCGCTCAA CACGTCATTC AACAATTGGG 1321 CTACCCCAAG GCGGCTCCAC CCTTGGCAAT GTTTAGCAAG CCAATTTGGA CAACCTGGGC 1381 ACGCTATAAA ATGGATATCG ATCAAGCTCA AACGCTGGCG TTTGCCCAAG AAATTATCGA 1441 CCAACAGTAT CCATATTCGG TGCTAGAGAT CGACGATCGC TGGCAAACTG CTTATGGCGA 1501 TTTAGAATTC GATCGGCGCA AGTTTCCCGA TCCCAAGGCT ATGGTCGATC AATTGCATCA 1561 GCTTGGCTAT AAAGTAACCT TGTGGATTCC ACCATTTTTC GATCCAAAGA GCGCGGCTTT 1621 TGCTGAAGCT ACTGCTAATG GCTATTTGGT CAAACATCCC GCCAACGATC AACCGTATTT 1681 GACCCGTTGG TGGCAAGGTT GGGGCGGTTT GTTGGATGTC TCGAATCCTG CTGCCTTGGC 1741 TTGGTGGCAA GCAGGTTTGG AGCGCTTGCA AACCTTGTAT GGCATCGATG GTTTTAAATT 1801 TGACGGCGCT GAAGGGAATT TTCTACCCGC CGAAGCCAAA ACCCATCTGC CGATGACTCC 1861 CAACCAATAT AGTGATCGCT ATGTGGCTTT TGTGGCCAAA TCGTGGCAAT GGACAGAGGT 1921 ACGAACTGGT TGGCGCTCGC AACAGCAACC AATCTTCTTC CGCGAATGGG ACAAATGGAG 1981 TCGTTGGGGC ATGGACAATG GCTTGCATGC GGTCGTTACC CAAGCGCTTG CGATGAGCGT 2041 GATCGGTTAT CCCTATGTGC TGCCCGATAT GATCGGCGGC AACGCCTATA ACGGTGAATT 2101 TCCCGAGCGC GAGTTGCTGA TTCGTTGGAC GCAAGTCACG GCATTATTGC CAGCGATGCA 2161 ATTTTCAATC GCGCCATGGC AGTACGATGT AGAAACCAGC CAGATTTGCC AGCGCTATGC 2221 TCAATTGCAC GCTGAGCTAG AGCCATACAT TGCCGAATTG GTGCAAGCGA CCATCACCGA 2281 TGGTACGCCT TTAGTTCGGC CTTTGTGGTG GCACTATCCC GACGATGCCA GCACGCGCTT 2341 TATTGGTGAT CAATGGTTGT TTGGCGAGCA ATACTTGGTT GCGCCAATGC TCCAAGCCAA 2401 CCACTACCAA CGTGACATTT ATTTGCCTGA AGGTGGCTGG CGCGATTATT GGACTGGCGA 2461 GAAATTCGAG GGTGAAACCT GGCTCTACAA TTATCCTGCG CCCTTAGAAA CCCTGCCGTT 2521 GTTCGAGCGG CTGTGGTAGG ACCCAGCTTT CTTGTACAAA GTTGGCATTA TAAGAAAGCA 2581 TTGCTTATCA ATTTGTTGCA ACGAACAGGT CACTATCAGT CAAAATAAAA TCATTATTTG 2641 CCATCCAGCT GATATCCCCT ATAGTGAGTC GTATTACATG GTCATAGCTG TTTCCTGGCA 2701 GCTCTGGCCC GTGTCTCAAA ATCTCTGATG TTACATTGCA CAAGATAAAA TAATATCATC 2761 ATGAACAATA AAACTGTCTG CTTACATAAA CAGTAATACA AGGGGTGTTA TGAGCCATAT 2821 TCAACGGGAA ACGTCGAGGC CGCGATTAAA TTCCAACATG GATGCTGATT TATATGGGTA 2881 TAAATGGGCT CGCGATAATG TCGGGCAATC AGGTGCGACA ATCTATCGCT TGTATGGGAA 2941 GCCCGATGCG CCAGAGTTGT TTCTGAAACA TGGCAAAGGT AGCGTTGCCA ATGATGTTAC 3001 AGATGAGATG GTCAGACTAA ACTGGCTGAC GGAATTTATG CCTCTTCCGA CCATCAAGCA 3061 TTTTATCCGT ACTCCTGATG ATGCATGGTT ACTCACCACT GCGATCCCCG GAAAAACAGC 3121 ATTCCAGGTA TTAGAAGAAT ATCCTGATTC AGGTGAAAAT ATTGTTGATG CGCTGGCAGT 3181 GTTCCTGCGC CGGTTGCATT CGATTCCTGT TTGTAATTGT CCTTTTAACA GCGATCGCGT 3241 ATTTCGTCTC GCTCAGGCGC AATCACGAAT GAATAACGGT TTGGTTGATG CGAGTGATTT 3301 TGATGACGAG CGTAATGGCT GGCCTGTTGA ACAAGTCTGG AAAGAAATGC ATAAACTTTT 3361 GCCATTCTCA CCGGATTCAG TCGTCACTCA TGGTGATTTC TCACTTGATA ACCTTATTTT 3421 TGACGAGGGG AAATTAATAG GTTGTATTGA TGTTGGACGA GTCGGAATCG CAGACCGATA 3481 CCAGGATCTT GCCATCCTAT GGAACTGCCT CGGTGAGTTT TCTCCTTCAT TACAGAAACG 3541 GCTTTTTCAA AAATATGGTA TTGATAATCC TGATATGAAT AAATTGCAGT TTCATTTGAT 3601 GCTCGATGAG TTTTTCTAAT CAGAATTGGT TAATTGGTTG TAACACTGGC AGAGCATTAC 3661 GCTGACTTGA CGGGACGGCG CAAGCTCATG ACCAAAATCC CTTAACGTGA GTTACGCGTC 3721 GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT 3781 TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT 3841 GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT 3901 ACCAAATACT GTTCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC 3961 ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA 4021 GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG 4081 CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG 4141 ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG 4201 GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA 4261 CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT 4321 GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG 4381 GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTT 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