LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2081..2177) /label=attL2 /note="attL2 (c)" misc_signal complement(2192..2211) /note="T7 Promoter/priming site (c)" primer_bind 2219..2235 /note="M13 Reverse priming site" gene 2348..3157 /note="Kanamycin resistance gene" rep_origin 3278..3951 /note="pUC origin" vector join(2089..3954,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 671..691 /note="TEV site" misc_feature 692..2074 /note="BGH30_B01b" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg cACCCAGGCC GTGGACACCG GCCCGCAAAA 721 AACCTTGCAG GTCTACACCA CCGCCAAAGA CACAAAATTG CGTCTCACCT TGAGCGATAA 781 ACTCTCGCTC AAACCCGCGC AACAGGCGCC GGAAACCGAC ACCGCCATTT TCGTCAACCC 841 GGACAAGCGC TACCAAACCC TGCTCGGTAT TGGCGGCGCT ATCACCGATG CCAGCGCCGA 901 GGTGTATGCA ACACTCGATC AAAAAACGCA GCGGGAATTT ATGCAGGCTT ACTATGACCA 961 GGACAAGGGT CTGGGCTACA GCCTGCTGCG CACCACCATC CACAGCTCCG ACTTTGCCAG 1021 CAGCAGCTAC ACCTATATCG AAGAGGGCGA CAAAGCGCTT AACACCTTTG ATATAACGCC 1081 GGATAAAAAA CACCGCATCC CCATGATCAA GCAGGCCATC CGGGCCGCCG GCGGCAGCAT 1141 CACCACCTAC GCCAGCCCCT GGAGCGCACC GGCGTTTATG AAAGACACCA ACAATATGCT 1201 GCAAGGCGGC AAGCTGCTGC CCGAATACCG CGACACCTGG GCCACCTACT TCACCAAATT 1261 TATCCGCGCC TATGAGCAGG AAGGCATTCC GATTTGGGGG ATTACCGTAC AAAACGAACC 1321 CATGGCCAAG CAAACCTGGG AATCGATGAT TTTCACCGCG GAAGAAGAGC GCGATTTCCT 1381 CAAAAACCAC CTGGGCCCGA TCATGCACAC ATCCGGCCTG GGCGATAAAA AAATCATCGT 1441 GTGGGATCAC AACCGCGACC TGATCACGCA CCGCGCCAAC ACCATCCTCA GCGATCCCGA 1501 AGCGGCTAAA TATGTCTGGG GCGTGGGCTT CCACTGGTAC GAAACCTGGA CCGGCGGCCA 1561 ACCCATGTTC GGCAACGTGG CCGAAGTCAA GCAGGCATTC CCGCATATCA ACCTGCTGCT 1621 CACCGAAGCC ACGGTGGAAA AATTTGATGC GGCGCGCTAC CACCACTGGC CCAATGCCGA 1681 ACGCTACGGC AACTCCATCG TCAATGACTT CAACGCCGGC GCGGCAGGCT GGACCGACTG 1741 GAATATGCTG CTGGACGATA AAGGCGGCCC CAACCATGTC AGCAATTTCT GCTTCGCCCC 1801 CATTCACGCC GACAGCAAAA CCGGCAAGCT GATTTACACC CCGACCTATT ACTACCTCGG 1861 CCACTTCTCG CGCTTTATCC GCCCTGAGGC GCAGCGGGTC AGTGCGGTCA GCAGCCGCAG 1921 CGTGCTGCAA ACCACGTCGT TTATCAACGC CGATAACCAA CTGGCAACGG TGGTCTTGAA 1981 TTTAAGCGAT GCCGCCGTGA CCTATCGCTT TTATATAGGC ACACAGGAAA CCACCGTTAC 2041 TATCCCCGCG CGCGCCATCC AAACGCTGGT GTATTAGGAC CCAGCTTTCT TGTACAAAGT 2101 TGGCATTATA AGAAAGCATT GCTTATCAAT TTGTTGCAAC GAACAGGTCA CTATCAGTCA 2161 AAATAAAATC ATTATTTGCC ATCCAGCTGA TATCCCCTAT AGTGAGTCGT ATTACATGGT 2221 CATAGCTGTT TCCTGGCAGC TCTGGCCCGT GTCTCAAAAT CTCTGATGTT ACATTGCACA 2281 AGATAAAATA ATATCATCAT GAACAATAAA ACTGTCTGCT TACATAAACA GTAATACAAG 2341 GGGTGTTATG AGCCATATTC AACGGGAAAC GTCGAGGCCG CGATTAAATT CCAACATGGA 2401 TGCTGATTTA TATGGGTATA AATGGGCTCG CGATAATGTC GGGCAATCAG GTGCGACAAT 2461 CTATCGCTTG TATGGGAAGC CCGATGCGCC AGAGTTGTTT CTGAAACATG GCAAAGGTAG 2521 CGTTGCCAAT GATGTTACAG ATGAGATGGT CAGACTAAAC TGGCTGACGG AATTTATGCC 2581 TCTTCCGACC ATCAAGCATT TTATCCGTAC TCCTGATGAT GCATGGTTAC TCACCACTGC 2641 GATCCCCGGA AAAACAGCAT TCCAGGTATT AGAAGAATAT CCTGATTCAG GTGAAAATAT 2701 TGTTGATGCG CTGGCAGTGT TCCTGCGCCG GTTGCATTCG ATTCCTGTTT GTAATTGTCC 2761 TTTTAACAGC GATCGCGTAT TTCGTCTCGC TCAGGCGCAA TCACGAATGA ATAACGGTTT 2821 GGTTGATGCG AGTGATTTTG ATGACGAGCG TAATGGCTGG CCTGTTGAAC AAGTCTGGAA 2881 AGAAATGCAT AAACTTTTGC CATTCTCACC GGATTCAGTC GTCACTCATG GTGATTTCTC 2941 ACTTGATAAC CTTATTTTTG ACGAGGGGAA ATTAATAGGT TGTATTGATG TTGGACGAGT 3001 CGGAATCGCA GACCGATACC AGGATCTTGC CATCCTATGG AACTGCCTCG GTGAGTTTTC 3061 TCCTTCATTA CAGAAACGGC TTTTTCAAAA ATATGGTATT GATAATCCTG ATATGAATAA 3121 ATTGCAGTTT CATTTGATGC TCGATGAGTT TTTCTAATCA GAATTGGTTA ATTGGTTGTA 3181 ACACTGGCAG AGCATTACGC TGACTTGACG GGACGGCGCA AGCTCATGAC CAAAATCCCT 3241 TAACGTGAGT TACGCGTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC 3301 TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT 3361 ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG 3421 CTTCAGCAGA GCGCAGATAC CAAATACTGT TCTTCTAGTG TAGCCGTAGT TAGGCCACCA 3481 CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC 3541 TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA 3601 TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC 3661 GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA 3721 AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG 3781 GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG 3841 ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG 3901 CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTT //