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FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(1841..1937) /label=attL2 /note="attL2 (c)" misc_signal complement(1952..1971) /note="T7 Promoter/priming site (c)" primer_bind 1979..1995 /note="M13 Reverse priming site" gene 2108..2917 /note="Kanamycin resistance gene" rep_origin 3038..3711 /note="pUC origin" vector join(1849..3714,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 671..691 /note="TEV site" misc_feature 692..1834 /note="BGH27_B01c" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg cCAAAAATTT GAGCAACTCG CTAAAACACC 721 GCAAATGGGT TGGAATAGCT GGAATACCTT CGGCTGCAAC GTGGATGAAA AAATGATTCG 781 CGCAATGGCC GATGCCATGG TCACATCCGG TATGAAGGCT GCCGGTTATG AATACATTAA 841 TATTGACGAT TGCTGGCACG GCGAGCGCGA TAAAAATGGC TTTATCCAGG CGGATAAAAA 901 ACATTTCCCT TCGGGCATGA AAGCACTTGC TGATTACGTG CATGCCAAAG GTTTGAAATT 961 GGGGATTTAT TCCGATGCCG GTAATACCAC CTGTGCCGGC CGCCCCGGCA GTCGCGGCCA 1021 TGAATACCAG GACGCACTGA CCTACGCGAG TTGGGGTATC GATTACGTAA AATACGATTG 1081 GTGCGATACC CAGGATATTA ATCCCAAGTC CGCCTACGCC ACCATGCGCG ATGCCATCCA 1141 TAAAGCGGGC CGCCCCATGT TGTTCAGTAT TTGCGAATGG GGCGATAACC AGCCCTGGGA 1201 ATGGGCGCAG GATGTCGGCC ATTCCTGGCG CACAACCGGC GACATCTATC CCTGTTGGAA 1261 CTGCGAACAC AACCACGGCT CCTGGTCTTC CTTTGGGGTG TTGCCCATCC TCGACAAACA 1321 AGCGGGCTTG CGTAAATACG CCGGCCCCGG CCACTGGAAT GACATGGATA TGATGGAAGT 1381 GGGCAACGGC ATGACCGAGG AAGAAGATCG CGCGCATTTC TCACTCTGGG CATTTATGGC 1441 ATCACCGCTG ATTGCCGGCA ATGACCTGCG CAACATGAGC GACACCACCC GCGCTATCCT 1501 GACCCACAAG GAAACCATTG CGATTAACCA GGACAAACTG GGCATCCAGG CCATGAAGTG 1561 GATCGATGAA GGCGACCTGG AGATTTATAT CAAGCCATTG GAAAAAGGCC ATTACGCTGT 1621 GCTGTTCCTC AATCGTGCCG ATGATGCCAT GGACTATCGT TTTGACTGGT CTTTCCATTA 1681 CATGAAAGAC GATATCAGCA AGCACGAAAT TTTCTTTGAT AAGCAGGCCT TTAACTGGCG 1741 CAATATCTGG AATGGTGAAA CAGGCTCTAC AAAAGAGGTG CTGAATATCA AGGTGCCAGC 1801 GCACGGTGTC GTGGTATTGC GTTTAAGCCC GCGTTAGGAC CCAGCTTTCT TGTACAAAGT 1861 TGGCATTATA AGAAAGCATT GCTTATCAAT TTGTTGCAAC GAACAGGTCA CTATCAGTCA 1921 AAATAAAATC ATTATTTGCC ATCCAGCTGA TATCCCCTAT AGTGAGTCGT ATTACATGGT 1981 CATAGCTGTT TCCTGGCAGC TCTGGCCCGT GTCTCAAAAT CTCTGATGTT ACATTGCACA 2041 AGATAAAATA ATATCATCAT GAACAATAAA ACTGTCTGCT TACATAAACA GTAATACAAG 2101 GGGTGTTATG AGCCATATTC AACGGGAAAC GTCGAGGCCG CGATTAAATT CCAACATGGA 2161 TGCTGATTTA TATGGGTATA AATGGGCTCG CGATAATGTC GGGCAATCAG GTGCGACAAT 2221 CTATCGCTTG TATGGGAAGC CCGATGCGCC AGAGTTGTTT CTGAAACATG GCAAAGGTAG 2281 CGTTGCCAAT GATGTTACAG ATGAGATGGT CAGACTAAAC TGGCTGACGG AATTTATGCC 2341 TCTTCCGACC ATCAAGCATT TTATCCGTAC TCCTGATGAT GCATGGTTAC TCACCACTGC 2401 GATCCCCGGA AAAACAGCAT TCCAGGTATT AGAAGAATAT CCTGATTCAG GTGAAAATAT 2461 TGTTGATGCG CTGGCAGTGT TCCTGCGCCG GTTGCATTCG ATTCCTGTTT GTAATTGTCC 2521 TTTTAACAGC GATCGCGTAT TTCGTCTCGC TCAGGCGCAA TCACGAATGA ATAACGGTTT 2581 GGTTGATGCG AGTGATTTTG ATGACGAGCG TAATGGCTGG CCTGTTGAAC AAGTCTGGAA 2641 AGAAATGCAT AAACTTTTGC CATTCTCACC GGATTCAGTC GTCACTCATG GTGATTTCTC 2701 ACTTGATAAC CTTATTTTTG ACGAGGGGAA ATTAATAGGT TGTATTGATG TTGGACGAGT 2761 CGGAATCGCA GACCGATACC AGGATCTTGC CATCCTATGG AACTGCCTCG GTGAGTTTTC 2821 TCCTTCATTA CAGAAACGGC TTTTTCAAAA ATATGGTATT GATAATCCTG ATATGAATAA 2881 ATTGCAGTTT CATTTGATGC TCGATGAGTT TTTCTAATCA GAATTGGTTA ATTGGTTGTA 2941 ACACTGGCAG AGCATTACGC TGACTTGACG GGACGGCGCA AGCTCATGAC CAAAATCCCT 3001 TAACGTGAGT TACGCGTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC 3061 TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT 3121 ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG 3181 CTTCAGCAGA GCGCAGATAC CAAATACTGT TCTTCTAGTG TAGCCGTAGT TAGGCCACCA 3241 CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC 3301 TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA 3361 TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC 3421 GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA 3481 AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG 3541 GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG 3601 ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG 3661 CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTT //