LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2312..2408) /label=attL2 /note="attL2 (c)" misc_signal complement(2423..2442) /note="T7 Promoter/priming site (c)" primer_bind 2450..2466 /note="M13 Reverse priming site" gene 2579..3388 /note="Kanamycin resistance gene" rep_origin 3509..4182 /note="pUC origin" vector join(2320..4185,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 671..691 /note="TEV site" misc_feature 692..2305 /note="BGH18_B05b" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg cGAGTCACCA AAGCAAGGTC AAAAAGTTGT 721 TGGGTATTTT CCTTCGTGGG GCATTTACGG ACGTAATTAT CAAGTTGCAG ACATTGATGC 781 ATCAAAGCTA ACTCATTTAA ACTACGCTTT CGCTGATATT TGCTGGAATG GAAAACATGG 841 AAACCCTTCT ACTCACCCCG ATAATCCAAA TAAACAAACG TGGGACTGTA AAGAATCTGG 901 TGTACCACTG CAAAATAAAG AAGTCCCTAA TGGTACTCTC GTACTCGGTG AACCATGGGC 961 CGATGTCACA AAATCTTATC CTGGCTCAGG TACAACTTGG GAAGATTGTG ATAAATACGC 1021 ACGCTGCGGA AATTTCGGCG AACTGAAACG ATTAAAAGCT AAGTATCCTC ACTTAAAAAC 1081 AATTATTTCT GTTGGTGGCT GGACTTGGTC TAACCGCTTT TCTGACATGG CCGCTGATGA 1141 AAAAACAAGA AAAGTATTCG CTGAATCTAC AGTCGATTTC CTACGCGAAT ATGGATTTGA 1201 CGGCGTTGAT TTAGATTGGG AATATCCGGG CGTAGAAACG ATTCCTGGTG GTAGTTATCG 1261 TCCTGAAGAT AAGCAAAACT TCACCCTACT CCTTCAAGAA GTTCGAAATG CATTAACGAA 1321 AGCTGGTGCA GAAGATGGCA AACAATATTT ACTAACAATC GCATCAGGTG CAAGCCAACG 1381 TTATGCCGAT CATACAGAGC TAAAGAAAAT CTCTCAAATA CTGGATTGGA TTAATATTAT 1441 GACATATGAT TTCCACGGTG GATGGGAAGC TACTTCTAAC CATAATGCAG CTCTATACAA 1501 AGATCCAAAT GACCCAGCAG CGGATACGAA ATTTTACGTA GATGGTGCGA TAGACATTTA 1561 TACAAATGAA GGGGTTCCAG CAGATAAACT CGTATTAGGT GTACCATTTT ACGGACGCGG 1621 ATGGAAAAGT TGTGGAAAAG AAAATAACGG ACAATACCAA CCTTGTAAAC CAGGTAGTGA 1681 TGGAAAGCTC GCTTCTAAAG GTACTTGGGA TGATTATTCT ACCGGTGACA CAGGTGTATA 1741 CGATTATGGT GATTTAGCGG CTAATTATGT TAATAAAAAT GGTTTTGTTC GCGGATGGAA 1801 CGATATAGCG AAAGTACCTT ATTTATATAA TGCGACTACA GGTACATTTA TTAGCTACGA 1861 TGATAATGAA TCTATGAAAT ATAAAACAGA CTATATAAAA ACAAAAGGTT TAAGTGGCGC 1921 AATGTTTTGG GAGCTTAGCG GAGATTGCCG TACAAGTCCA AAATATAGTT GTAGTGGACC 1981 AAAATTACTT GATACGTTAG TAAAAGAATT ACTAGGTGGA CCTATTACTC AAAAAGATAC 2041 TGAGCCACCA ACAAACGTAA AAAATATTGT TGTTACAAAT AAAAATTCAA GCTCAGTTCA 2101 ATTAAACTGG AATGCATCTA CTGATAACGT AGGAGTTACG GAATATGAAA TTACTGCTGG 2161 GGAAGAGAAA TGGAGTACAA CAACAAATAA CATTACAATT AAAAACTTAA AATCTAATAC 2221 AGAATACAAA TTTTCAATAA TAGCAAAAGA TGCCGCTGGG AATAAATCAC AGCCTACTTC 2281 TATTATTGTA AAAACAGACG ATGCTTAGGA CCCAGCTTTC TTGTACAAAG TTGGCATTAT 2341 AAGAAAGCAT TGCTTATCAA TTTGTTGCAA CGAACAGGTC ACTATCAGTC AAAATAAAAT 2401 CATTATTTGC CATCCAGCTG ATATCCCCTA TAGTGAGTCG TATTACATGG TCATAGCTGT 2461 TTCCTGGCAG CTCTGGCCCG TGTCTCAAAA TCTCTGATGT TACATTGCAC AAGATAAAAT 2521 AATATCATCA TGAACAATAA AACTGTCTGC TTACATAAAC AGTAATACAA GGGGTGTTAT 2581 GAGCCATATT CAACGGGAAA CGTCGAGGCC GCGATTAAAT TCCAACATGG ATGCTGATTT 2641 ATATGGGTAT AAATGGGCTC GCGATAATGT CGGGCAATCA GGTGCGACAA TCTATCGCTT 2701 GTATGGGAAG CCCGATGCGC CAGAGTTGTT TCTGAAACAT GGCAAAGGTA GCGTTGCCAA 2761 TGATGTTACA GATGAGATGG TCAGACTAAA CTGGCTGACG GAATTTATGC CTCTTCCGAC 2821 CATCAAGCAT TTTATCCGTA CTCCTGATGA TGCATGGTTA CTCACCACTG CGATCCCCGG 2881 AAAAACAGCA TTCCAGGTAT TAGAAGAATA TCCTGATTCA GGTGAAAATA TTGTTGATGC 2941 GCTGGCAGTG TTCCTGCGCC GGTTGCATTC GATTCCTGTT TGTAATTGTC CTTTTAACAG 3001 CGATCGCGTA TTTCGTCTCG CTCAGGCGCA ATCACGAATG AATAACGGTT TGGTTGATGC 3061 GAGTGATTTT GATGACGAGC GTAATGGCTG GCCTGTTGAA CAAGTCTGGA AAGAAATGCA 3121 TAAACTTTTG CCATTCTCAC CGGATTCAGT CGTCACTCAT GGTGATTTCT CACTTGATAA 3181 CCTTATTTTT GACGAGGGGA AATTAATAGG TTGTATTGAT GTTGGACGAG TCGGAATCGC 3241 AGACCGATAC CAGGATCTTG CCATCCTATG GAACTGCCTC GGTGAGTTTT CTCCTTCATT 3301 ACAGAAACGG CTTTTTCAAA AATATGGTAT TGATAATCCT GATATGAATA AATTGCAGTT 3361 TCATTTGATG CTCGATGAGT TTTTCTAATC AGAATTGGTT AATTGGTTGT AACACTGGCA 3421 GAGCATTACG CTGACTTGAC GGGACGGCGC AAGCTCATGA CCAAAATCCC TTAACGTGAG 3481 TTACGCGTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA 3541 TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT 3601 GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG 3661 AGCGCAGATA CCAAATACTG TTCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA 3721 CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG 3781 TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA 3841 GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC 3901 CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA 3961 GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC 4021 AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG 4081 TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC 4141 CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTT //