LOCUS       pDONR/Zeo
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FEATURES             Location/Qualifiers
     misc_feature    complement(268..295)
                     /note="rrnB T2 transcription termination sequence (c)"
     misc_feature    complement(427..470)
                     /note="rrnB T1 transcription termination sequence (c)"
     primer_bind     537..552
                     /note="M13 Forward (-20) priming site"
     misc_recomb     570..668
                     /label=attL1
                     /note="attL1"
     misc_recomb     complement(1991..2087)
                     /label=attL2
                     /note="attL2 (c)"
     misc_signal     complement(2102..2121)
                     /note="T7 Promoter/priming site (c)"
     primer_bind     2129..2145
                     /note="M13 Reverse priming site"
     gene            2258..3067
                     /note="Kanamycin resistance gene"
     rep_origin      3188..3861
                     /note="pUC origin"
     vector          join(1999..3864,1..651)
                     /source="pDONR%99221"
                     /type="Donor Vector"
     misc_feature    671..691
                     /note="TEV site"
     misc_feature    692..1984
                     /note="BGH13_B01b1"
ORIGIN      
          1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
         61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
        121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
        181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
        241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
        301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
        361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
        421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
        481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
        541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC
        601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA
        661 AGCAGGCTct gaaaacttgt actttcaagg cCAACCGCGA ACCGCGTTCG TTCATTTGTT
        721 CGAATGGCAG TGGAACGATA TTGCTAGCGA GTGCGAAAAT GTACTGGGGC CAAAAGGCTA
        781 TGCGGCAGTG CAGGTATCCC CACCCCAAAA ATCGGTTAGT AGCAGCCAAT GGTGGAGTCG
        841 TTACCAACCT GTTAGCTATG CCATAGAGGG CCGTTCTGGC AATCGCAACG AATTCGCCAG
        901 CATGGTAAGC CGGTGTAAAA ATGCTGGGGT GGATATATAC GTAGATGCCG TAATTAATCA
        961 TATGGCTGCC GGCGGGCGCT ATTACCCAGA AGTGCCTTAC TACGCAGAGC ATTTTCACAC
       1021 GTGCTACGGC CAAATTGATT ACGGTAACCG CTGGCAGGTA CAAAACTGTG ACTTGGTAGG
       1081 GCTAAACGAT TTAGCAACAG AACAAGACTA TGTACGCACA AAAATAGCCA ATTACTTAAA
       1141 CGACTTAACT AACCTTGGCG TAGCTGGCTT CCGCTTTGAT GCTGCGAAAC ATATCCCCGT
       1201 GGGTGACATT GCTGCAATTA AAGCAAAACT TATTGGCAAC CCGTATATAT TCCAAGAAGT
       1261 GATAGGTGCG CCGGGCGAGC CCATCCAACC ATCAGAATAT ACCTACTTAG CAGACGTAAC
       1321 AGAGTTTAAC TTTACCAAAT CGGTTAGCCA TTTTTTTAAA GGGCGCGGAC CAATTAAAGA
       1381 GTTAAAAAAT ATTGGTACTT GGGGCGGTTG GGTTGCAAGT GCAGACGCCG TAACCTTTGT
       1441 TGCCAACCAC GATAACCAGC GCCAGGATAC AAACAACACC ATTACCCATA AAGATGGTGA
       1501 TAACCGCAAT ACTATGGCGC ACGTATTTAT GCTGGGCTGG CCCTATGGGT ACCCTAAAGT
       1561 AATGTCTAGT TATGAGTGGT ACGATCACGA CCAAGGCCCT CCCGCCCACG GTGCTAGCAG
       1621 CTGTAACAGC GGCTGGTTAT GTGAACACCG CACGCCAGCT ATCGCCAATA TGGTTGCTTT
       1681 TCGCAATTTC ACTGCAGACG AATTTCGCGT AACCAACTAT TGGGATAACG GCAACAATCA
       1741 AATAGCTTGG GGCCGAGGCG GCAAAGGGTT TGTAGTTATA AATATGGAAG GCAACAATTT
       1801 AACCCGTACA TTTCAAACAG GGATGCCCGC TGGCCGTTAT TGCGACATTA TTTCGGGTAA
       1861 CTACAACGCG TCTAATAAAA GTTGTTCTGG CAATATTATA GATGTAGATA ACAATGGCAA
       1921 CGCAGTAATC ACCGCTTACT ACCGCAACGG TGCCGCAATT CATGTAGGCG CTATTGTTGA
       1981 AGACTAGGAC CCAGCTTTCT TGTACAAAGT TGGCATTATA AGAAAGCATT GCTTATCAAT
       2041 TTGTTGCAAC GAACAGGTCA CTATCAGTCA AAATAAAATC ATTATTTGCC ATCCAGCTGA
       2101 TATCCCCTAT AGTGAGTCGT ATTACATGGT CATAGCTGTT TCCTGGCAGC TCTGGCCCGT
       2161 GTCTCAAAAT CTCTGATGTT ACATTGCACA AGATAAAATA ATATCATCAT GAACAATAAA
       2221 ACTGTCTGCT TACATAAACA GTAATACAAG GGGTGTTATG AGCCATATTC AACGGGAAAC
       2281 GTCGAGGCCG CGATTAAATT CCAACATGGA TGCTGATTTA TATGGGTATA AATGGGCTCG
       2341 CGATAATGTC GGGCAATCAG GTGCGACAAT CTATCGCTTG TATGGGAAGC CCGATGCGCC
       2401 AGAGTTGTTT CTGAAACATG GCAAAGGTAG CGTTGCCAAT GATGTTACAG ATGAGATGGT
       2461 CAGACTAAAC TGGCTGACGG AATTTATGCC TCTTCCGACC ATCAAGCATT TTATCCGTAC
       2521 TCCTGATGAT GCATGGTTAC TCACCACTGC GATCCCCGGA AAAACAGCAT TCCAGGTATT
       2581 AGAAGAATAT CCTGATTCAG GTGAAAATAT TGTTGATGCG CTGGCAGTGT TCCTGCGCCG
       2641 GTTGCATTCG ATTCCTGTTT GTAATTGTCC TTTTAACAGC GATCGCGTAT TTCGTCTCGC
       2701 TCAGGCGCAA TCACGAATGA ATAACGGTTT GGTTGATGCG AGTGATTTTG ATGACGAGCG
       2761 TAATGGCTGG CCTGTTGAAC AAGTCTGGAA AGAAATGCAT AAACTTTTGC CATTCTCACC
       2821 GGATTCAGTC GTCACTCATG GTGATTTCTC ACTTGATAAC CTTATTTTTG ACGAGGGGAA
       2881 ATTAATAGGT TGTATTGATG TTGGACGAGT CGGAATCGCA GACCGATACC AGGATCTTGC
       2941 CATCCTATGG AACTGCCTCG GTGAGTTTTC TCCTTCATTA CAGAAACGGC TTTTTCAAAA
       3001 ATATGGTATT GATAATCCTG ATATGAATAA ATTGCAGTTT CATTTGATGC TCGATGAGTT
       3061 TTTCTAATCA GAATTGGTTA ATTGGTTGTA ACACTGGCAG AGCATTACGC TGACTTGACG
       3121 GGACGGCGCA AGCTCATGAC CAAAATCCCT TAACGTGAGT TACGCGTCGT TCCACTGAGC
       3181 GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT
       3241 CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA
       3301 GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT
       3361 TCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA
       3421 CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC
       3481 CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG
       3541 TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG
       3601 TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG
       3661 CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT
       3721 TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC
       3781 AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT
       3841 TTGCTGGCCT TTTGCTCACA TGTT
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